RUSKI-201 dihydrochloride is a potent and specific Hedgehogacyltransferase(Hhat) inhibitor with anIC₅₀of 0.20 μM. RUSKI-201 dihydrochloride is able to block Hh signaling from Shh overexpressing cells and inhibits Hh palmitoylation. RUSKI-201 dihydrochloride is potential Hhat chemical probe in cells and can used in studies of Hhat catalytic function^[1].

RUSKI-201 (0.5-25 μM; 48 hours) has no effect on cell viability at concentrations >25 μM in Shh-Light2 cells derived from NIH3T3 cells stably transfected with a Gli-responsive firefly luciferase HEK-293 cells stably overexpressing Shh^[1].
RUSKI-201 inhibits signaling in H520, Panc-1, and MCF-7 coculture with Shh-Light2 cells (IC₅₀=4.8±0.60 μM, 7.8±1.3 μM, and 8.5±0.65 μM, respectively)^[1]RUSKI-201 (0.01-10 μM; 24 hours) induces a selective inhibition of Shh palmitoylation and does not affect global palmitoylation levels in HEK-293 Shh⁺cells treated with RUSKI-201 followed by YnPal and functionalized with AzTB^[1].

430.43

C₂₀H₂₉Cl₂N₃OS

2320262-09-5

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.