OTUB1/USP8-IN-1 - 半岛电竞官方网址

您好，欢迎来到半岛电竞官方网址！ [ 登录] [ 免费注册]

半岛游戏平台官网入口网址

原料

发布求购

位置：首页> 半岛bd体育手机客户端库> OTUB1/USP8-IN-1

立即咨询

咨询类型：

采购售后

*姓名：

*电话：

*单位：

Email：

*留言内容：

请详细说明您的需求。

*验证码：

OTUB1/USP8-IN-1

本半岛bd体育手机客户端不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。

包装与价格：

包装	价格(元)
5mg	电议
10mg	电议
25mg	电议
50mg	电议

半岛bd体育手机客户端介绍

OTUB1/USP8-IN-1 是一种有效的双重 OTUB1/USP8抑制剂，抑制 OTUB1 和 USP8 的 IC₅₀值分别为 0.17 和 0.28 nM。OTUB1/USP8-IN-1 可用于癌症的研究。

生物活性

OTUB1/USP8-IN-1 is a potent dualOTUB1/USP8inhibitor withIC₅₀values of 0.17 and 0.28 nM forOTUB1and USP8, respectively. OTUB1/USP8-IN-1 can be used in research ofcancer^[1].

IC₅₀& Target

IC50: 0.17 nM (OTUB1) and 0.28 nM (USP8)^[1]

体外研究
(In Vitro)

OTUB1/USP8-IN-1 (compound 61; 10 nM-10 μM; 72 h) has antiproliferative effects in KRAS-WT (H1975, EBC-1, H1703) and KRAS-mutated (H23, A549) NSCLC cell lines^[1].
OTUB1/USP8-IN-1 (500 nM; 24 h) decreases in protein levels of both UBE2N and EGFR in H1975 cells^[1].

Cell Viability Assay^[1]

Cell Line:	KRAS-WT (H1975, EBC-1, H1703) and KRAS-mutated (H23, A549) NSCLC cell lines
Concentration:	10 nM-10 μM
Incubation Time:	72 hours
Result:	Inhibited cell proliferative with IC₅₀values of 118, 145, 172, 431, and 1004 nM for H1975, H1703, EBC-1, H23, and A549 cells, respectively.

Western Blot Analysis^[1]

Cell Line:	H1975 cells
Concentration:	500 nM
Incubation Time:	24 hours
Result:	Decreased the levels of both UBE2N and EGFR in a dose-dependent manner.

体内研究
(In Vivo)

OTUB1/USP8-IN-1 (compound 61; 10 nM-10 μM; 72 h) decreases the tumor burden in the H1975 xenograft mouse model^[1].

Animal Model:	Female BALB/c nude mice with H1975 xenografts (5 weeks of age)^[1]
Dosage:	5 mg/kg
Administration:	Intraperitoneal injection; QD and BID, for 2 weeks
Result:	Reduced total tumor weight and average tumor volume in an over twofold with BID dosing.