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Camptothecin
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Camptothecin图片
CAS NO: 7689-03-4
包装与价格：
包装 价格(元)
10 mM * 1 mL in DMSO 电议
100mg 电议
500mg 电议
1 g 电议
5 g 电议

半岛bd体育手机客户端 名称
喜树碱
Campathecin
(S)-(+)-Camptothecin
CPT
半岛bd体育手机客户端 介绍
Camptothecin (CPT)，一种生物碱，是一种 DNA topoisomerase I (Topo I)抑制剂，其 IC50值为 679 nM。Camptothecin (CPT) 对结直肠癌、乳腺癌、肺癌和卵巢癌具有强大的抗肿瘤活性，通过改变人类癌细胞中的 miRNA表达模式来调节 hypoxia-inducible factor-1α (HIF-1α)活性。
生物活性

Camptothecin (CPT), a kind of alkaloid, is aDNAtopoisomeraseI (Topo I) inhibitorwith anIC50of 679 nM[1]. Camptothecin (CPT) exhibits powerful antineoplastic activity against colorectal, breast, lung and ovarian cancers, modulateshypoxia-inducible factor-1α (HIF-1α)activity by changingmicroRNAs(miRNA)expression patterns in humancancercells[2][3].
IC50& Target[3]

Topoisomerase I

679 nM (IC50)

Camptothecins
体外研究
(In Vitro)

High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward Camptothecin (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC50values of 0.089 μM and 0.067 μM, respectively[4].
Camptothecin (0.5 μM; 6 and 24 hours) reduces desferrioxamine-activated VEGF expression.
Camptothecin (0.5 μM; 8 to 24 hours) strongly prevents the desferrioxamine-dependent HIF-1a accumulation[2].

Cell Viability Assay[4]

Cell Line: MCF7 (Luminal subtype) and HCC1419 (HER2 subtype)
Concentration: 0.1 μM to 5 μM
Incubation Time: 72 hours
Result: High TOP1 enzymatic activity MCF7 (Luminal subtype) and HCC1419 (HER2 subtype) show high sensitivity toward CPT (0.1 μM to 5 μM; 72 hours) treatment, exhibiting the IC50values of 0.089 μM and 0.067 μM, respectively.

RT-PCR[2]

Cell Line: HeLa and HEK293 cell lines
Concentration: 0.5 μmol/L
Incubation Time: 6 and 24 hours
Result: Reduces desferrioxamine-activated VEGF expression in both cell lines after 6 and 24 hours of treatment, whereas in normoxic condition camptothecin does not affect the VEGF mRNA level.

Western Blot Analysis[2]

Cell Line: HeLa and HEK293 cell lines
Concentration: 0.5 μmol/L
Incubation Time: 8 to 24 hours
Result: Strongly prevents the desferrioxamine-dependent HIF-1a accumulation after 8 to 24 hours, whereas it does not affect HIF-1b levels.
体内研究
(In Vivo)

Camptothecin (2 mg/kg every other day) treats mice, has developed numerous pulmonary metastases.
Treatment with both kinase inhibitor of nuclear factor-kappaB-1 (KINK-1) and Camptothecin led to a statistically significant reduction in the number of pulmonary metastases[5].

Animal Model: C57BL6 mice (injected with B16F10 melanoma cells)[5]
Dosage: 2 mg/kg
Administration: every other day, after 19 days
Result: Has developed numerous pulmonary metastases.
Clinical Trial
分子量

348.35
性状

Solid
Formula

C20H16N2O4
CAS 号

7689-03-4
中文名称

喜树碱
结构分类
  • Alkaloids
  • Quinoline Alkaloids
来源
  • Plants
  • Nyssaceae
  • Camptotheca acuminataDecne.
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶解性数据
In Vitro:

1M NaOH : 10 mg/mL(28.71 mM;ultrasonic and adjust pH to 11 with NaOH)

DMSO : 6.25 mg/mL(17.94 mM;Need ultrasonic)

配制储备液
浓度溶剂体积质量 1 mg 5 mg 10 mg
1 mM 2.8707 mL 14.3534 mL 28.7068 mL
5 mM 0.5741 mL 2.8707 mL 5.7414 mL
10 mM 0.2871 mL 1.4353 mL 2.8707 mL
*

请根据半岛bd体育手机客户端 在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的半岛bd体育手机客户端 失效。
储备液的保存方式和期限：-80℃, 6 months; -20℃, 1 month (protect from light)。-80℃ 储存时，请在 6 个月内使用，-20℃ 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照In Vitro方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

  • 1.

    请依序添加每种溶剂： 15% Cremophor EL85% Saline

    Solubility: 10 mg/mL (28.71 mM); Suspended solution; Need ultrasonic

* 以上所有助溶剂都可在本网站选购。
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