化学性质

Physical Appearance	A solid
Storage	Store at -20°C
M.Wt	248.36
Cas No.	519-02-8
Formula	C15H24N2O
Synonyms	Sophocarpidine;Matridin-15-one;Vegard;α-Matrine
Solubility	≥12.42 mg/mL in DMSO; ≥47.2 mg/mL in EtOH; ≥50.3 mg/mL in H2O with gentle warming and ultrasonic
Chemical Name	(41S,7aS,13aR,13bR)-dodecahydro-1H-dipyrido[2,1-f:3',2',1'-ij][1,6]naphthyridin-10(41H)-one
Canonical SMILES	O=C1N(C[C@@]2([H])[C@@]3([H])[C@]4([H])CCCN3CCC2)[C@]4([H])CCC1
运输条件	蓝冰运输或根据您的需求运输。
一般建议	为了使其更好的溶解，请用37℃加热试管并在超声波水浴中震动片刻。不同厂家不同批次半岛bd体育手机客户端 溶解度各有差异，仅做参考。若实验所需浓度过大至半岛bd体育手机客户端 溶解极限，请添加助溶剂助溶或自行调整浓度。溶液形式一般不宜长期储存，请尽快用完。

资料参考

IC50 Value: 540 μg/ml (inhibit gastric cancer cell line MNK45, MTT) [1] Matrine is an alkaloid found in plants from the Sophora genus. It has a variety of pharmacological effects, including anti-cancer effects, and action as a kappa opioid receptor and ?-receptor agonist. in vitro: MTT assay showed that the matrine was able to inhibit gastric cancer cell line MNK45 in a dose-dependent manner. The concentration required for 50% inhibition (IC50) was found to be 540 μg/ml. This anti-tumor function was achieved through modulation of the NF-κB, XIAP, CIAP, and p-ERK proteins expression in cell line MNK45. Matrine induces apoptosis of human NSCLC cells with anti-apoptotic factors inhibited and dependent on caspase activity. In addition, we found that matrine increases the phosphorylation of p38 but not its total protein, and inhibition of the p38 pathway with SB202190 partially prevents matrine-induced apoptosis. Furthermore, matrine generates reactive oxygen species (ROS) in a dose- and time-dependent manner, which is reversed by pretreatment with N-acetyl-L-cysteine (NAC) [2]. in vivo: Oral administration of matrine (200, 100 and 50 mg/kg) significantly attenuated isoproterenol-induced cardiac necrosis and left ventricular dysfunction [3]. high dose of matrine significantly reduced the mortality rate of mice with LPS administration. Treatment with matrine improved LPS-induced lung histopathologic changes, alleviated pulmonary edema and lung vascular leak, inhibited MPO and MDA activity,and reduced the production of inflammatory mediators including TNF-α, IL-6 and HMGB1 [4]. Toxicity: N/A Clinical trial: N/A

试验操作

细胞实验 [1]:
细胞系	MNK45胃癌细胞
溶解方法	溶于水、乙醇、氯仿、甲苯和苯。为了获得更高浓度，可以将离心管在37℃加热10分钟和/或在超声波浴中震荡一段时间。原液可以在-20℃以下储存几个月。
反应条件	0.05, 0.10, 0.25, 和 0.50 mg/ml孵育48小时
应用	Matrine能够剂量依赖性地抑制MKN45细胞生长，其抑制50％所需的浓度为0.5mg / ml。 还发现，经matrine（0.05，0.1,0.25,0.50 mg / ml）孵育后，MKN45细胞中的IKKa，IjBa，IjBb，phospho-IjBa蛋白水平明显高于对照组细胞。 此外，低剂量matrine处理的MKN45细胞中的p-ERK蛋白表达水平明显高于高剂量matrine处理的细胞。
动物实验 [2]:
动物模型	成年雄性BALB/c小鼠
剂量	25, 50 或者 100 mg/kg，腹腔给药
应用	Matrine治疗可显著降低LPS诱导的小鼠死亡率，高剂量matrine（100mg / kg）治疗组3天内累积死亡率（55％）明显低于LPS组（80％）。然而，当小鼠以25mg / kg和50mg / kg的低剂量接受matrine治疗时，没有观察到保护作用。Matrine治疗也可显著改善肺损伤，如肺水肿、炎症细胞浸润肺组织和肺泡、以及肺泡损伤。对照组和matrine组肺结构无明显变化。
注意事项	请测试所有化合物在室内的溶解度，实际溶解度和理论值可能略有不同。这是由实验系统的误差引起的，属于正常现象。
References: [1]. Luo C, et al. Inhibition of matrine against gastric cancer cell line MNK45 growth and its anti-tumor mechanism. Mol Biol Rep. 2012 May;39(5):5459-64. [2]. Zhang B, et al. Antiinflammatory effects of matrine in LPS-induced acute lung injury in mice. Eur J Pharm Sci. 2011 Dec 18;44(5):573-9.