| 包装 | 价格(元) |
| 10mM (in 1mL DMSO) | 电议 |
| 5mg | 电议 |
| 10mg | 电议 |
| 25mg | 电议 |
DAPI, as a DNA-specific probe, can form a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA and nonfluorescent intercalative complexes with double-stranded nucleic acids.
Cell lines |
bone marrow-derived adherent cells |
Preparation Method |
After three passages, bone marrow-derived adherent cells were incubated with 50 μg/ml DAPI (Molecular Probes, Invitrogen, Carlsbad, CA) at 37℃ and 5% CO2 for 2 h. BM-MSCs were treated with trypsin (Gibco) for 3 min to generate a single-cell suspension, washed three times with DMEM, resuspended in serum-free DMEM, counted, and stored on ice until transplantation. Fluorescence from DAPI labeling on BM-MSCs and cell viability were evaluated for 1, 2, 3, 4, and 8 weeks after cell labeling. |
Reaction Conditions |
50 μg/ml; at 37℃ and 5% CO2 for 2 h |
Applications |
After three passages in culture, the isolated cell population became homogeneous, showing a monolayer consisting of adherent cells displaying further traits of MSCs, including a typical fibroblast-like morphology and increased proliferation. |
Animal models |
Brain tumor xenograft in nude mice |
Preparation Method |
Tissue sections for in vivo analysis were collected from animals 3 days after PBS, PAM-RG4/pJDK, or PAM-RG4/pJDK-apoptin injection and immediately paraffinized before further analysis using the DeadEnd(TM) Fluorometric TUNEL System. Following the given protocol, samples were analyzed using fluorescence microscopy to detect the localized green fluorescence of apoptotic cells and blue fluorescence (DAPI) of cell nuclei. |
Dosage form |
3 days after injection; 0.5-10μg/ml |
Applications |
Apoptosis, chromatin condensation, which is caused by the degradation of nuclear membrane proteins, by using DAPI staining. however, when applied in vivo, the experimental group did not show any signs of side effects, indicating a sufficiently low level of cytotoxicity, although it showed similar DAPI and TUNEL assay results. |
| 文献引用 | |
| 半岛bd体育手机客户端 描述 | DAPI, as a DNA-specific probe, can form a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA and nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI, as a chromosome and nuclear stain, has maximum excitation ultraviolet (UV) light wavelength with 358 nm and emission in the blue range with 461 nm.[1] DAPI is usually used for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry.[2]DAPI is a good substrate of hOCT1 with a Michaelis constant of 8.94 μM.[6]. In vitro, DAPI (1.43μM) can measure mitochondrial permeability transition and mitochondrial membrane depolarization by combining with Annexin V(FITC)and the potentiometric fluorescent dye, tetramethylrhodamine methyl ester (TMRM).[3]In vitro, to evaluate the DAPI fluorescence, SSC buffer (pH 7.2), containing 0.01, 0.015, 0.02, 0.025, 0.03, 0.035, 0.04% SDS or Triton X-100 and 2 μg/mL of DAPI dye, there was found that a linear relationship between DAPI fluorescence and Triton X-100 concentrations ranging from 0.01 to 0.04%.[4]In addition, when the concentration of DAPI was increased from 0.4 μM to 400 μM, the total signal intensity increased, implying an increase in the concentration of DAPI molecules bound to the chromosomes.[5]In vitro experiment it shown that treatment with NR 1 μg/mL, DAPI 5 μg/mL, ORO 0.3% (v/v) and CV 0.5% (v/v) provided optimal linearity and coverage of signals over a range of cell densities (corresponding to 10-100% cell confluence).[7]. References: |
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