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DAF 2DA(DAF-2DA)
本半岛bd体育手机客户端 不向个人销售,仅用作科学研究,不用于任何人体实验及非科研性质的动物实验。
DAF 2DA(DAF-2DA)图片
CAS NO: 205391-02-2
包装与价格:
包装 价格(元)
100 μg 电议
250 μg 电议
500 μg 电议
1mg 电议

半岛bd体育手机客户端 介绍
DAF 2DA (DAF-2DA) (5,6-Diaminofluorescein diacetat) 是最广泛用于 NO 测量的探针。
Cas No. 205391-02-2
别名 5,6-Diaminofluorescein diacetat
Canonical SMILES O=C1OC2(C3=C(OC4=C2C=CC(OC(C)=O)=C4)C=C(OC(C)=O)C=C3)C5=C1C=C(N)C(N)=C5
分子式 C24H18N2O7
分子量 446.41
溶解度 Soluble in DMSO
储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
半岛bd体育手机客户端 描述

DAF 2DA is most widely probe for NO measurement.

Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF 2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF 2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).

[1]. Wany A, et al. Localization of Nitric Oxide in Wheat Roots by DAF Fluorescence. Methods Mol Biol. 2016;1424:39-47.

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