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Fl-DIBO
本半岛bd体育手机客户端 不向个人销售，仅用作科学研究，不用于任何人体实验及非科研性质的动物实验。
Fl-DIBO图片
CAS NO: 1407523-31-2
包装: 10mg
市场价: 10800元

半岛bd体育手机客户端 介绍
Fl-DIBO (fluorogenic dibenzocyclooctyne) 是一种对叠氮化合物 (azide) 具有选择性、高灵敏度的荧光探针。Fl-DIBO 可以与叠氮化合物快速反应形成新的高荧光产物，其最大发射波长为 469 nm，激发波长为 363 nm。Fl-DIBO 可用于标记重氮标签蛋白而无可检测的背景信号干扰。
Cas No. 1407523-31-2
分子式 C19H12O3
分子量 288.3
储存条件 Store at -20°C
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.
Shipping Condition Evaluation sample solution : ship with blue ice
All other available size: ship with RT , or blue ice upon request
半岛bd体育手机客户端 描述

Fl-DIBO (fluorogenic dibenzocyclooctyne) is a selective and high sensitivity fluorescent probe toazidecompounds. Fl-DIBO can react rapidly with azide compounds to form new highly fluorescent products with a maximum emission wavelength of 469 nm and excitation wavelength of 363 nm. Fl-DIBO can be used to label diazo-tagged proteins without detectable background signal interference[1][2].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling diazo-labeled proteins[2][3]:
1. Resuspend the protein with 1×PBS (pH 7.4) and incubate with NHS-activated ester (25 mM in DMSO) overnight at room temperature.
2. Labeled proteins are co-incubated with Fl-DIBO (25-500 μM) at 37 °C for 18 h.
3. Protein SDS-PAGE gel analysis and in-gel fluorescence imaging analysis with Λex=365 nm, Λem=480 nm.
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