MC3482是一种特异性sirtuin5(SIRT5)抑制剂。
货号:ajcx13560
CAS:N/A
分子式:C33H38N4O8
分子量：618.68
溶解度:DMSO : ≥ 130 mg/mL (210.12 mM);H2O :< 0.1 mg/mL (insoluble)
纯度：98%
存储：Store at -20°C
库存： 现货

Background:

MC3482 is a specific sirtuin5 (SIRT5) inhibitor.

MC3482 inhibits sirtuin5. 50 μM MC3482 inhibits SIRT5 desuccinylating activity without affecting SIRT5 intracellular expression levels. Autophagy and mitophagy increase in SIRT5-silenced cells and in WT cells treated with MC3482 and decrease in SIRT5-overexpressing cells[1].

[1]. Polletta L, et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy. 2015;11(2):253-70.

Protocol:

Cell experiment:	MDA-MB-231 and C2C12 cells are treated with 50μM of SIRT5 inhibitor MC3482 for 24 h. Ammonia levels are measured in the culture medium every other day[1].
参考文献: [1]. Polletta L, et al. SIRT5 regulation of ammonia-induced autophagy and mitophagy. Autophagy. 2015;11(2):253-70.