5-IAF是荧光素的偶氮乙酰胺衍生物。
CAS：63368-54-7
分子式：C22H14INO6
分子量：515.25
纯度：98%
存储：Store at -20°C

Background:

5-IAF is an idoacetamide derivate of fluoresceine.

8 ?M GSH sample solution is derivatized with 8, 80, 200, 400, 800, 1600, 2000, 2500 ?M 5-IAF, respectively and analyzed with CE-LIF. The highest signal intensity is obtained with 400 and 800 ?M 5-IAF[2].

[1]. Salvatore Sotgia, et al. Plasma L-Ergothioneine Measurement by High-Performance Liquid Chromatography and Capillary Electrophoresis after a Pre-Column Derivatization with 5-Iodoacetamidofluorescein (5-IAF) and Fluorescence Detection. PLoS One. 2013; 8(7): e70374. [2]. Yan Wang, et al. Determination of free and protein-bound glutathione in HepG2 cells using capillary electrophoresis with laser-induced fluorescence detection. J Chromatogr A. 2009 Apr 17;1216(16):3533-7.