Toll-like receptor 3 (TLR3) agonist
CAS：24939-03-5
分子式：C19H27N7O16P2
分子量：671.41
纯度：98%
存储：Store at -20°C

Background:

Synthetic double-stranded RNA (dsRNA); Toll-like receptor 3 (TLR3) agonist. Aids generation of stable, mature dendritic cells in combination with TLR8 agonist CL 075. Transfection into NIT-1 β cells induces β-cell apoptosis. Immunostimulant.

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine

Reference:

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[2]. Rouas et al (2004) Poly(I:C) used for human dendritic cell maturation preserves their ability to secondarily secrete bioactive IL-12. Int.Immunol. 16 767 PMID: 15096480
[3]. West et al (2011) TLR signalling augments macrophage bactericidal activity through mitochondrial ROS. Nature 472 476 PMID: 21525932
[4]. Spranger et al (2010) Generation of Th1-polarizing dendritic cells using the TLR7/8 agonist CL075. J.Immunol. 185 738 PMID: 20511554