Resazurin sodium salt是一种蓝色非荧光染料,广泛用作活细胞的代谢指示剂,通过被还原成荧光产物试卤灵,测量细菌和真核细胞活力。
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Resazurin sodium salt is a blue non-fluorescent dye,is widely used as a metabolic indicator for living cells
体外活性
Resazurin inhibits the growth of a broad range of N. gonorrhoeae isolates, including those resistant to multiple antibiotics[1]
体内活性
In vivo resazurin did not limit the colonisation of mice with N. gonorrhoeae following vaginal infection.?The ineffectiveness of resazurin in vivo is likely due to its interaction with serum albumin, which completely diminishes its antimicrobial activity[1].
细胞实验
Overnight broth cultures were used to inoculate FB containing resazurin sodium salt, resorufin sodium salt , resorufin acetate [Marker Gene Technologies, Eugene, OR; dissolved in dimethyl sulfoxide (DMSO)], resorufin butyrate or resorufin pentyl ether . In select experiments, FB was also supplemented with 3 g/dL bovine serum albumin (BSA) . Immediately following inoculation and 24 h later, cultures were serially diluted and were plated onto chocolate II agar. Plates were incubated at 37 °C in 5% CO2 and individual colonies were enumerated. The limit of detection was 100 CFU/mL[1].
Cas No.
62758-13-8
分子式
C12H6NNaO4
分子量
251.173
别名
刃天青钠;Diazoresorcinol (sodium);Resazurin sodium salt;Resazurin sodium
储存和溶解度
DMSO:150 mg/mL (597.21 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years