N1-Methylpseudouridine 是一种甲基假尿苷，翻译性能比 5 mC 和 5 mC/N1-Methylpseudouridine高。通过提高核糖体密度，mRNA 中的 N1-Methylpseudouridine 以 eIF2α 依赖性和独立机制增强翻译。

N1-Methylpseudouridine is a methylpseudouridine and enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density in mRNA.

In HEK293T cells, Incorporation of N1-Methylpseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA .?Incorporation of N1-Methylpseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation.?In addition, polysome formation and growth on the N1-Methylpseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate[1].

mRNAs containing the N1-Methylpseudouridine modification alone and/or in combination with 5-methylcytidine (m5C) outperformed the current state-of-the-art pseudouridine (Ψ) and/or m5C/Ψ-modified mRNA platform by providing up to ~44-fold (when comparing double modified mRNAs) or ~13-fold (when comparing single modified mRNAs) higher reporter gene expression upon transfection into cell lines or mice, respectively. We show that (m5C/)N1-Methylpseudouridine-modified mRNA resulted in reduced intracellular innate immunogenicity and improved cellular viability compared to (m5C/)Ψ-modified mRNA upon in vitro transfection[2].

13860-38-3

C10H14N2O6

258.23

1-Methylpseudouridine;N1-甲基-假尿苷

H2O:40 mg/mL (154.90 mM)
DMSO:100 mg/mL (387.25 mM)
Powder: -20°C for 3 years
In solvent: -80°C for 2 years