BMSpep-57 hydrochloride is a potent and competitive macrocyclic peptide inhibitor ofPD-1/PD-L1interactionwith anIC₅₀of 7.68 nM. BMSpep-57 hydrochloride binds toPD-L1withK_ds of 19 nM and 19.88 nM in MST and SPR assays, respectively. BMSpep-57 hydrochloride facilitates T cell function by in creasingIL-2production in PBMCs^[1].

IC50: 7.68 nM (PD-1/PD-L1 interaction)^[1]

In a ELISA competition assay, BMSpep-57 inhibits PD-1/PD-L1 binding up to 98.1% 300 nM. And it shows a concentration dependent inhibition of PD-1/PD-L1 binding with an IC₅₀of 7.68 nM^[1].
BMSpep-57 induced high levels of IL-2 at 1 μM and 500 nM concentrations in SEB-stimulated peripheral blood mononuclear cells^[1].
BMSpep-57 (0.2-10 μM; 24 hours) does not show any effect on the Jurkat, CHO and HepG2 cells’ viability at the various concentrations tested^[1].

1904.63

Solid

C₈₉H₁₂₇ClN₂₄O₁₉S

{mercaptoacetic acid}-Phe-Ala-Asn-Pro-His-Leu-Ser-Trp-Ser-Trp-{norleucine}-{norleucine}-Arg-Cys-Gly (Sulfide bridge:mercaptoacetic acid 1-Cys15)

{mercaptoacetic acid}-FANPHLSWSW-{norleucine}-{norleucine}-RCG (Sulfide bridge:mercaptoacetic acid 1-Cys15)

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)