Cell lines

The human cholangiocarcinoma (CCA) cell lines HCCC9810 and QBC939

Preparation method

The solubility of this compound in DMSO is >12.3mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

0, 30, 60, 90, 120, or 150 μM; 24, 48, or 72 h; 37℃

Applications

In CCA cells, kaempferol significantly inhibited the viability and proliferation of CCA cells in a time- and dose-dependent way. Kaempferol reduced the number of colonies and induced apoptosis in a dose-dependent way. Kaempferol also reduced the migration and invasion ability of CCA cells in a dose-dependent manner.

Animal models

subcutaneous xenograft nude mice model

Dosage form

20 mg/kg/day; daily for 3 weeks; i.p.

Application

In subcutaneous xenograft nude mice model, kaempferol significantly inhibited the growth of the tumor. The number of Ki-67-positive tumor cells was lower in kaempferol-treated group than that in the control group. Kaempferol reduced the average volume and number of foci per mouse.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.