棰勬湡鐢ㄩ€擖/strong>

鏈瘯鍓傜洅搴旂敤鍙屾姉浣撳す蹇冮叾鑱斿厤鐤硶妫€娴婽7 RNA Polymerase鐨勬畫鐣欍€侟/span>

妫€娴嬪師鐞咟/strong>

T7 RNA Polymerase (T7 RNA鑱氬悎閰 )涓洪噸缁凟.coli琛ㄨ揪鐨勫櫖鑿屼綋T7 DNA缂栫爜鐨勮泲鐧斤紝鏄竴绉嶉珮搴︾壒寮傛€ц瘑鍒玊7鍚姩瀛愬簭鍒楃殑DNA渚濊禆鐨?'鈫?' RNA鑱氬悎閰躲€俆7 RNA鑱氬悎閰跺彲浠ュ惈鏈塗7鍚姩瀛愬簭鍒楃殑鍗曢摼鎴栧弻閾綝NA涓烘ā鏉匡紝NTP涓哄簳鐗╋紝鍚堟垚涓庡惎鍔ㄥ瓙涓嬫父鐨勫崟閾綝NA鎴栧弻閾綝NA妯℃澘閾句簰琛ョ殑RNA 銆侟/span>

鏈瘯鍓傜洅搴旂敤鍙屾姉浣撳す蹇冮叾鑱斿厤鐤硶 (澶瑰績ELISA)妫€娴婽7 RNA Polymerase鐨勫惈閲忥紝鐢ㄦ姉T7 RNA Polymerase鐨勫崟鍏嬮殕鎶椾綋鍖呰閰舵爣鏉匡紝褰㈡垚鍥虹浉鎶椾綋锛屽悜鍥虹浉鎶椾綋寰瓟鏉夸腑鍔犲叆T7 RNA Polymerase鏍″噯鍝佸拰寰呮祴鏍峰搧锛岀粡杩囨礂娑ゅ悗锛屽姞鍏ヨ荆鏍硅繃姘у寲鐗╅叾鏍囪鐨勯叾鏍囪瘯鍓傦紝褰㈡垚 鈥滃寘琚姉浣 -鎶楀師 -閰舵爣妫€娴嬫姉浣 鈥濆鍚堢墿锛岀粡杩囨礂娑ゅ悗鍔犲叆鏄捐壊娑叉樉鑹诧紝鏄捐壊娑插湪杈ｆ牴杩囨哀鍖栫墿閰剁殑鍌寲涓嬭浆鍖栨垚钃濊壊骞跺湪閰哥殑浣滅敤涓嬫渶缁堣浆鍖栨垚榛勮壊锛岄鑹茬殑娣辨祬涓庢牱鍝佷腑T7 RNA Polymerase鐨勯噺鍛堟鐩稿叧銆侟/span>

涓昏缁勬垚鎴愬垎

	缁勫垎	鐩栬壊	DD3504-01
BOX1	T7 RNA Polymerase鏍″噯鍝?8,000 ng/ml)	绾㈣壊	100 渭l
BOX2	棰勫埗閰舵爣鏉 (鍖呰anti-T7 RNA Polymerase鐨勫崟鍏嬮殕鎶椾綋 )	-	12 脳 8瀛旓紝96瀛擖/span>
	鏍峰搧绋€閲婃恫	-	30 ml 脳 2
	娴撶缉娲楁钉娑?20 脳)	-	30 ml
	閰舵爣璇曞墏绋€閲婃恫	-	12 ml
	閰舵爣璇曞墏(100 脳)	閫忔槑	120 渭l
	鏄捐壊娑秉/span>	-	12 ml
	缁堟娑秉/span>	-	6 ml
	灏佹澘鑶淈/span>	-	3寮燑/span>
	璇存槑涔?/span>	-	1鍐孅/span>

璇存槑锛氭湰璇曞墏鐩掍笉鍙笌鍏朵粬鍟嗗搧鍖栬瘯鍓傜洅娣风敤銆侟/span>

闇€瑕佷絾鏄湭鎻愪緵鐨勮瘯鍓備笌鑰楁潗锛欬/span>

>鍘荤瀛愭按鎴栬捀棣忔按

>鎸崱鍣?/span>

>娲楁澘鏈裹/span>

>寰噺绉绘恫鍣ㄤ笌閰嶅鐏弻鏋ご

>鎭掓俯绠辨垨姘存荡閿匋/span>

>閰舵爣浠?/span>

>鍔犳牱妲键/span>

>鍚告按绾伏/span>

鍌ㄥ瓨鏉′欢鍙婃湁鏁堟湡

1銆丅OX1浜?30 锝 -15鈩冨偍瀛橈紝閬垮厤寮哄厜鐩村皠锛屾湁鏁堟湡12涓湀锛汓/span>

BOX2浜? 锝 8鈩冨偍瀛橈紝閬垮厤寮哄厜鐩村皠锛屾湁鏁堟湡12涓湀銆侟/span>

2銆佸寘琚潯鎷嗗皝浣跨敤鍚庯紝搴斿皢鍓╀綑鍖呰鏉″瘑灏侊紝鍦? 锝 8鈩冨偍瀛橈紝鍦ㄦ湁鏁堟湡鍐呬娇鐢ㄣ€侟/span>

3銆丅OX 1浣跨敤鍚庤鍙婃椂鏀惧洖鍒?30 锝 -15鈩冨偍瀛橈紝閬垮厤鍙嶅鍐昏瀺锛屽湪鏈夋晥鏈熷唴浣跨敤銆侟/span>

4銆丅OX 2鍏朵粬缁勫垎浣跨敤鍚庤鍙婃椂鏀惧洖鍒? 锝 8鈩冩潯浠讹紝鍦ㄦ湁鏁堟湡鍐呬娇鐢ㄣ€侟/span>

5銆佷骇鍝佹壒鍙峰強澶辨晥鏃ユ湡锛氳浜у搧澶栧寘瑁呮爣绛俱€侟/span>

妫€楠屾柟娉旤/strong>

1銆?/strong>瀹為獙鍓嶅噯澶嘃/strong>

(1)灏嗚瘯鍓傜洅浠庡喎钘忕幆澧冧腑鍙栧嚭锛屾牎鍑嗗搧浠庡喎鍐荤幆澧冧腑鍙栧嚭锛屾斁缃娓?18 锝 28鈩?骞宠　鑷冲皯 30 min銆侟/span>

(2)1 脳娲楁钉娑查厤鍒讹細灏嗘祿缂╂礂娑ゆ恫(20 脳)鐢ㄥ幓绂诲瓙姘存垨钂搁姘?0鍊嶇█閲婏紝娣峰寑澶囩敤銆備緥濡?0 ml娴撶缉娲楁钉娑?20 脳)鐢?70 ml鍘荤瀛愭按鎴栬捀棣忔按绋€閲娿€侟/span>

(3)鏍″噯鍝佺殑閰嶅埗锛氬彇鏍″噯鍝佸師娑?8,000 ng/ml)绋€閲?00鍊嶅悗涓?0 ng/ml鐨勬祿搴︼紝鐒跺悗鍐嶇█閲?鍊嶄负鏍″噯鍝佺殑**涓偣(16 ng/ml)锛屽啀2鍊嶅€嶆瘮绋€閲婃垚8銆?銆?銆?銆?.5 ng/ml銆備负淇濊瘉瀹為獙缁撴灉鏈夋晥鎬э紝姣忔瀹為獙璇蜂娇鐢ㄦ柊閰嶅埗鐨勬牎鍑嗗搧婧舵恫銆侟/span>

鈻叉爣鍑嗗搧鍒濇浣跨敤鍚庤鍒嗚淇濆瓨

绉诲彇	鍔犲叆鑷颤br/>	閰嶆垚T7 RNA Polymerase鏍″噯鍝佹祿搴?br/>
10 渭l鐨?,000 ng/ml鐨勬牎鍑嗗搧	990 渭l鐨勬牱鍝佺█閲婃恫	80 ng/ml
500 渭l鐨?0 ng/ml鐨勬牎鍑嗗搧	800 渭l鐨勬牱鍝佺█閲婃恫	16 ng/ml
500 渭l鐨?6 ng/ml鐨勬牎鍑嗗搧	500 渭l鐨勬牱鍝佺█閲婃恫	8 ng/ml
500 渭l鐨? ng/ml鐨勬牎鍑嗗搧	500 渭l鐨勬牱鍝佺█閲婃恫	4 ng/ml
500 渭l鐨? ng/ml鐨勬牎鍑嗗搧	500 渭l鐨勬牱鍝佺█閲婃恫	2 ng/ml
500 渭l鐨? ng/ml鐨勬牎鍑嗗搧	500 渭l鐨勬牱鍝佺█閲婃恫	1 ng/ml
500 渭l鐨? ng/ml鐨勬牎鍑嗗搧	500 渭l鐨勬牱鍝佺█閲婃恫	0.5 ng/ml
500 渭l鐨勬牱鍝佺█閲婃恫	绌虹(瀵圭収瀛?	0 ng/ml

(4)1 脳閰舵爣璇曞墏鐨勯厤鍒讹細灏嗛叾鏍囪瘯鍓?100 脳)鐢ㄩ叾鏍囪瘯鍓傜█閲婃恫绋€閲婃垚1 脳閰舵爣璇曞墏锛屾牴鎹娴嬫暟纭畾绋€閲婁綋绉?姣忓瓟100 渭l)锛屼緥濡傛娴嬫暣鏉匡紝鐞嗚闇€瑕?0 ml锛屽疄闄呴厤鍒?1 ml 1 脳閰舵爣璇曞墏锛屽叾浠栨儏鍐垫牴鎹墍闇€妫€娴嬫暟鐞嗚鐢ㄩ噺锛屽閲?0%閰嶅埗銆侟/span>

2銆佸疄楠屾楠｜/strong>

(1)鍔犳牱锛氬厛鍦ㄩ叾鏍囨澘涓瘡瀛斿姞鍏?00 渭l鏍峰搧/鏍″噯鍝併€侟/span>

鈻插緟娴嬫牱鍝侀渶鐢ㄦ牱鍝佺█閲婃恫鑷冲皯绋€閲?鍊嶃€侟/span>

(2)瀛佃偛锛氱敤灏佹澘鑶滃皝鏉垮悗锛屾斁鍏?7鈩冩亽娓╁煿鍏荤瀛佃偛1 h銆侟/span>

(3)娲楁澘锛氬鑲插畬鎴愬悗锛屽皬蹇冩彮鍘诲皝鏉胯啘锛屽純鎺夊瓟鍐呮恫浣擄紝姣忓瓟鑷冲皯鍔犲叆300 渭l 1 脳娲楁钉娑诧紝闈欑疆娴告场30 s锛岃繛缁礂鏉?娆★紝鏈€鍚庝竴娆″敖閲忓幓闄ゆ畫鐣欐恫浣撱€侟/span>

(4)鍔犻叾鏍囪瘯鍓傦細浠ユ瘡瀛?00 渭l鍔犳牱閲忓姞鍏? 脳閰舵爣璇曞墏銆侟/span>

(5)閲嶅姝ラ2銆?銆侟/span>

(6)鏄捐壊锛氭寜姣忓瓟100 渭l鍔犳牱閲忓皢鏄捐壊娑插姞鍏ラ叾鏍囨澘锛岀敤灏佹澘鑶滃皝鏉垮悗锛屾斁鍏?7鈩冩亽娓╁煿鍏荤閬垮厜瀛佃偛15 min銆侟/span>

(7)缁堟/璇诲€硷細姣忓瓟鍔犲叆50 渭l缁堟娑诧紝杞昏交娣峰寑鍚庡嵆鍙敤閰舵爣浠娴嬪悇瀛斿湪450 nm鍗曟尝闀垮OD鍊笺€侟/span>

绠€鏄庢搷浣滅▼搴廃/strong>

璐ㄩ噺鎺у埗

妫€娴嬫椂锛屾牎鍑嗘洸绾跨浉鍏崇郴鏁癛²鈮?.99锛屽惁鍒欏疄楠屾棤鏁堛€侟/span>

缁撴灉璁＄畻

(1)鏍″噯鍝佸拰鏍峰搧瀛旀祴寰楃殑OD鍊兼墸闄ゅ鐓у瓟鐨凮D鍊肩敤浜庣粨鏋滆绠椼€侟/span>

(2)鍙栦互10涓哄簳鏍″噯鍝佹祿搴︾殑瀵规暟涓烘í鍧愭爣锛孫D鍊间负绾靛潗鏍囷紝閲囩敤鍥涘弬鏁版柟寮忔嫙鍚堢粯鍒舵牎鍑嗘洸绾裤€傚鏈夎缃瀛旓紝鍒欏簲鍙栧叾骞冲潎鍊艰绠椼€侟/span>

(3)灏嗘牱鍝佺殑OD鍊兼墸闄ゅ鐓у瓟鐨凮D鍊间唬鍏ユ牎鍑嗘洸绾跨殑鎷熷悎鏂圭▼寮忥紝璁＄畻鍑烘牱鍝佹祿搴︼紝鍗充负鏍峰搧鐨勫疄闄呮祿搴︺€傛渶浣庡畾閲忛檺(LOQ)=0.5 ng/ml锛屼綆浜?.5 ng/ml鎶ュ憡涓?lt;0.5 ng/ml銆傝嫢鏍峰搧OD鍊奸珮浜庢牎鍑嗘洸绾夸笂闄愶紝搴旈€傚綋绋€閲婂悗閲嶆祴锛岃绠楁祿搴︽椂搴斾箻浠ョ█閲婂€嶆暟銆傛鏍″噯鏇茬嚎浠呯敤浜庣ず鑼冿紝姣忔瀹為獙鍧囧簲鐢熸垚鏂扮殑鏍″噯鏇茬嚎銆侟/span>

浜у搧鎬ц兘鎸囨爣

1銆佺伒鏁忓害

LOD	LOQ
100 pg/ml	0.5 ng/ml

2銆佺簿瀵嗗害

鍒嗘瀽鍐呯簿瀵嗗害				鍒嗘瀽闂寸簿瀵嗗害
鏍峰搧	鎵规1	鎵规2	鎵规3	鎿嶄綔鑰?	鎿嶄綔鑰?	鎿嶄綔鑰?
n	8	8	8	8	8	8
骞冲潎鍊稽br/>	2.3295	1.9380	1.8819	2.2142	2.1607	2.2902
SD	0.0827	0.0828	0.0394	0.1085	0.0721	0.1494
CV	4%	4%	2%	5%	3%	7%

3銆佸洖鏀剁巼

鏍峰搧(n=3)	娴嬪緱娴撳害骞冲潎(ng/ml)	娴嬪緱娴撳害骞冲潎(ng/ml)	骞冲潎鍥炴敹鐜?	鍥炴敹鐜囪寖鍥?
楂楛br/>	8.6875	9.0643	105	101 - 110
	9.0066
	9.4987
涓?br/>	1.9855	2.0072	91	90 - 92
	2.0027
	2.0334
浣嶞br/>	0.7118	0.7461	102	97 - 111
	0.8122
	0.7144

娉ㄦ剰浜嬮」

(1)鎿嶄綔鍓嶄粩缁嗛槄璇讳娇鐢ㄨ鏄庝功锛屼弗鏍兼寜鐓ц瘯鍓傜洅璇存槑涔﹁繘琛屽疄楠屾搷浣溿€侟/span>

(2)閬垮厤鍦ㄦ伓鍔ｇ殑鐜(濡傚惈鏈?4娑堟瘨娑层€佹姘吀閽犮€侀吀纰辨垨涔欓啗绛夐珮娴撳害鑵愯殌鎬ф皵浣撳強鐏板皹鐨勭幆澧?鏉′欢涓嬭繘琛屽疄楠岋紝瀹為獙瀹ゆ秷姣掑簲鍦ㄥ疄楠岀粨鏉熷悗杩涜銆侟/span>

(3)璇曞墏鐩掑簲骞宠　鑷冲娓╀笅鏂瑰彲鎵撳紑浣跨敤锛岃瘯鍓備娇鐢ㄥ墠搴斿厖鍒嗘憞鍖€銆傚悇缁勫垎鍌ㄥ瓨涓庣敤娉曡涓ユ牸鎸夌収浣跨敤璇存槑锛屽垏鍕胯嚜琛屾洿鏀逛笌绋€閲娿€傚湪浣跨敤鍓嶏紝璇蜂弗鏍兼鏌ヨ瘯鍓傜洅鐨勬晥鏈熶笌鍖呰銆傚鏋滄晥鏈熻秴鏃舵垨鍖呰鐮存崯锛岃涓嶈鐢ㄤ簬瀹為獙鎿嶄綔銆傝瘯鍓傚湪鏈夋晥鏈熷唴浣跨敤鍚庯紝鍓╀綑璇曞墏瑕佸強鏃跺瘑灏侊紝鍙傜収璇存槑涔﹁繘琛屼繚瀛樸€侟/span>

(4)棰勫埗閰舵爣鏉垮彲鎷嗗嵏锛屾瘡娆″皢闇€鐢ㄦ暟閲忕殑鏉挎潯鍙栧嚭鍚庯紝鍏朵綑鏈敤鏉挎潯椤绘斁鍥為摑绠旇鍐呬簬2 锝 8鈩冨偍瀛樺緟鐢ㄣ€傛媶鍗告澘鏉℃椂锛屽垏鍕跨瑙﹀瓟搴曪紝浠ュ厤鎸囩汗銆佸埉鐥曠瓑褰卞搷涔嬪悗鐨勮鍊笺€傛礂鏉垮悗锛屽垏鍕挎斁缃お涔呴伩鍏嶉叾鏍囨澘骞茬嚗澶辨椿锛岄渶绔嬪嵆杩涜涓嬩竴姝ユ搷浣溿€侟/span>

(5)鍔犳牱鍝佹椂锛岄伩鍏嶄骇鐢熸皵娉★紝閬垮厤鏋ご瑙﹀強鏉垮簳锛岄€犳垚鍒棔褰卞搷璇诲€笺€侟/span>

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