QuantSeq Kit 鏄竴涓枃搴撳埗澶囪瘯鍓傜洅锛岀敱瀹冩瀯寤虹殑鏂囧簱鍙互鐢ㄤ簬Illumina鍜屾€籖NA鐨勮捣濮嬮噺浣庤嚦100 pg锛堝浜嶪llumina骞冲彴锛夊拰5ng锛?nbsp;瀵逛簬Ion Torrent骞冲彴锛夈€俀uantSeq閫傜敤浜庡弽澶嶆瀯寤轰綆璐ㄩ噺鐨凴NA鏂囧簱锛屽寘鎷現FPE鏍峰搧銆侟br/>Rapid Turnaround
绠€鍗曠殑宸ヤ綔娴佺▼鍙互灏嗘枃搴撳埗澶囨椂闂存帶鍒跺湪4.5灏忔椂浠ュ唴锛岃€岃繖宸茬粡鍖呮嫭浜嗘墜宸ユ搷浣滄椂闂淬€傞櫎鍘绘墜宸ユ搷浣滄椂闂达紝宸ヤ綔娴佺▼涓嶈秴杩?灏忔椂銆侟br/>楂樺害閾剧壒寮傛€?br/>QuantSeq淇濇寔浜嗛摼鐗瑰紓鎬?gt;99.9锛咃紝骞朵笖鍙互map鍒板熀鍥犵粍涓婄浉搴旂殑搴忓垪涓婏紝浠庤€屽彂鐜板拰瀹氶噺鍙嶄箟杞綍鏈拰铻嶅悎鍩哄洜銆侟br/>Mapping of TES
QuantSeq鍙互鍑嗙‘鏌ユ壘鍒癛NA3'绔殑poly(A) 锛屽洜姝ゅ彲浠ュ緱鍒板叧浜?'UTR鐨勭簿纭俊鎭€侟br/>Perfect For Gene Counting
姣忎釜杞綍鏈彧浜х敓涓€涓墖娈贷鍥犳娌℃湁蹇呰鍋氶暱搴︾殑鍧囦竴鍖栥€傝繖鏍峰彲浠ユ洿绮剧‘鍦扮‘瀹氬熀鍥犵殑琛ㄨ揪閲忥紝骞朵笖鍦ㄥ熀鍥犺〃杈惧拰eQTL鐮旂┒涓璔uantSeq鎴愪负鑺墖鍜屽父瑙凴NA-Seq鐨勬渶浣虫浛浠ｅ搧銆侟br/>
Cost Saving Multiplexing
QuantSeq鏂囧簱鐢ㄤ簬澶氶噸鏂囧簱鐨勬瀯寤恒€傚浜?nbsp;Illumina 鐨凲uantSeq3' mRNA-Seq 鏂囧簱鍒跺璇曞墏鐩掍腑鐨刡arcodes鍏佽鍦?nbsp;Illumina flow cell姣忔潯閫氶亾涓婃祴搴忕殑鏍锋湰澶氳揪96涓€傚浜嶪on Torrent鐨凲uantSeq3' mRNA-Seq鏂囧簱鍒跺璇曞墏鐩掍腑鐨刡arcodes鍏佽鍦↖on Torrent浠櫒涓婂杈?8涓牱鏈竴璧疯繘琛屾祴搴忋€傚姝ら珮鐨勬贩鏍风▼搴﹀彲浠ヨ妭绾︽垚鏈鍚孮uantSeq鐗瑰畾闀垮害鑺傜渷娴嬪簭绌洪棿涓€鏍枫€俀uantSeq鎵€璁捐鐢熶骇鐨勬彃鍏ョ墖娈靛ぇ灏忛€傜敤浜庣煭娴嬪簭鏈嶅姟锛圫R50锛孲R100锛夈€侟br/>鏈夊叧barcodes鍜岃鏄庡浣曚娇鐢ㄥ畠浠殑璇︾粏淇℃伅锛岃鍙傝€冮檮褰旸锛氭贩鏍凤紝QuantSeq for Ion Torrent鐢ㄦ埛鎸囧崡锛堢23椤碉級鍜岄檮褰旹:Multiplexing锛 QuantSeq for Illumina User Guide(绗?5椤?銆侟br/>绠€鍗曠殑鐢熺墿淇℃伅瀛﹀垎鏋忺br/>搴忓垪鍖归厤鏄畝鍗曠殑锛屽畠閫氳繃璺宠繃浜ゅ弶鐐规鏉ュ疄鐜般€傛祴搴忔搷浣滄槸鍦ㄨ浆褰曟湰鐨勬渶3'绔嚑涔庢病鏈夌粨鐐圭殑浣嶇疆浜х敓銆傛暟鎹鐞嗕篃鍙互鍔犻€熶娇鐢紝渚嬪Bowtie2浠ｆ浛TopHat2銆侟br/>Ion Torrent骞冲彴锛屾枃搴撴墍娴嬬殑搴忓垪闈犺繎Poly锛圓锛塕NA鐨?'绔€侟/h3>

璇ョ洅瀛愭湁涓ょ鐗堟湰銆傜脳涓増鏈殑NGS娴嬪畾鐨勫簭鍒楁槸闈犺繎 poly(A) 灏剧敓鎴愶紝鐩存帴鍙嶆槧浜唌RNA搴忓垪銆傚悓鏃舵彁渚涗簡Illumina鍜孅/span> Ion Torrent涓や釜骞冲彴鐨勭増鏈€俀uantSeq Reverse锛圧EV锛変粎閫傜敤浜嶪llumina骞冲彴銆備娇鐢ㄨ繖涓洅瀛愶紝鍦∟GS浠巔oly锛圓锛夊熬寮€濮嬭鍙朁/span>Read 1鏃跺彲浠ョ簿纭殑鏌ユ壘鍒?'绔紙3'UTR锛夈€傜劧鑰岋紝涓轰簡瀹炵幇澶氫釜鏂囧簱鍚屾椂娴嬪簭锛屾祴搴忓紩鐗╅渶瑕佽Custom Sequencing Primer锛圕SP锛屽寘鎷湪璇曞墏鐩掑唴锛夋墍鏇挎崲銆傝鍙栫殑Read 1鍙互鍙嶆槧cDNA搴忓垪銆侟/span>