Rac-GTP灏忛紶鍗曟姉 - 半岛电竞官方网址

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Rac-GTP灏忛紶鍗曟姉

鍨嬪彿:	Rac
璐у彿:	26903
鍝佺墝:	NewEast Biosciences
鍙傝€冧环鏍机	4800鍏?涓?/td>
鍖呰:	30寰崌
浜よ揣鏈烔	鐜拌揣

绱㈠彇璧勬枡鍙婃姤浠饵/span>

鍖呰涓庝环鏍硷細

璁㈣揣鍙饵/td>	绾害瑙勬牸	鍖呰	浠锋牸(鍏?
26903		30寰崌	4800

浜у搧鍚嶇О

Rac-GTP娲绘€ф姉浣掽br />Anti-Rac1-GTP Monoclonal Antibody

浜у搧浠嬬粛

璐у彿:26903

瑙勬牸:30 渭L

鍩哄洜绗﹀彿:RAC1

鎻忚堪:Anti-Rac1-GTP Monoclonal Antibody

鑳屾櫙浠嬬粛:Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).

鍏嶇柅鍘烔Recombinant full length protein of active Rac1

缁忚繃娴嬭瘯鐨勫簲鐢?IP, IHC, IF

鎺ㄨ崘绋€閲婃瘮:IP: 1 渭g for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250

娴撳害:1 mg/ml

瀹夸富:Mouse

褰㈠紡:Liquid

鍏嬮殕鎬?Monoclonal

浜氬瀷:IgM

绾害:Purified from ascites

闃茶厫鍓侜No

鎴愬垎:PBS (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

绉嶅睘鍙嶅簲鎬?Anti-Rac1-GTP monoclonal antibody recognizes active Rac1 from vertebrates.

鍌ㄥ瓨鏉′欢:Store at -20掳C. Avoid repeated freezing and thawing

鍏嶇柅娌夋穩/铔嬬櫧璐ㄥ嵃杩跟

Rac Activation Assay.MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti-Rac-GTP monoclonal antibody (Cat. # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti-Rac rabbit polyclonal antibody (Cat. # 21003). The bottom panel shows the Western blot with anti-Rac of the cell lysates used (5% of that used in the top panel).

鍏嶇柅鑽у厜:

Immunofluorescence for the active Rac using Anti-Rac1-GTP Mouse Monoclonal Antibody [26903] shows Rac-GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 oC methanol or 4% paraformaldehyde (fixation time 1 hr) and stained with antibody at 1: 1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti-mouse AlexaFluor488 and anti-rabbit AlexaFluor594 at 1: 1000.