hole Genome Amplification for Library Construction of Single Cells

鈥淪urePlex? [PicoPLEX] represents a major step forward in amplification technology鈥e selected SurePLEX due to its rapid protocol, highly representative amplification,and low allele dropout when compared to other amplification methods.鈥滭/p>

鈥揘ick Haan, founder and CEO of BlueGnome, an Illumina Company

姒傝堪

鏈変竴涓粏鑳炴墿澧濪NA 浜х敓涓€涓珮搴﹀彲鍐嶇敓鐨勬枃搴撶幇鍦ㄦ垚涓轰簡鍙兘锛丳icoPLEX WGA kit 鐢ㄤ簬璁捐浼樺寲鐢ㄤ簬鍗曟嫹璐濆熀鍥犵粍DNA鐨勬墿澧炪€傜畝鍗曟槗鐢ㄧ殑鍗曠鎿嶄綔娴佺▼鍑忓皯浜嗘墜鍔ㄦ搷浣滅殑澶辫锛屽緱鍒扮粨鏋滅殑鏃堕棿寰楀埌浜嗗ぇ骞呭害鐨勪紭鍖栵紝骞朵笖闄嶄綆浜嗚儗鏅€侾icoPLEX WGA kit 鍚屾牱鍙互琚敤浜庡垎绂诲ソ鐨勫熀鍥犵粍DNA锛岃寖鍥翠负15pg鍒?0ng涔嬮棿銆傚彲浠ュ簲鐢ㄤ簬Array CGH鍜宷-PCR.

*Protected by US Patent 8,206,913 and EU pending applications

PicoPLEX WGA kits 琚獻VF琛屼笟棰嗗鑰匓lueGnome鍏徃淇¤禆骞朵娇鐢紝鍦ㄥ叏涓栫晫鑼冨洿鍐呯敤浜嶱GS/PGD椤圭洰銆侟/p>

• 鍗曚釜缁嗚優璧峰鐨勬墿澧炲弽搴旓紙鎴?lt;15pg DNA锛堻/p>

• 鍗撹秺鐨勭瓑浣嶅熀鍥犻噸澶嶆€?/p>

• 绠€鍗曟槗鐢ㄧ殑鎿嶄綔娴佺▼

• 鍑嗙‘鍙潬鐨勭粨鏋滐紝涓嶈鏄姱鐗囪繕鏄疨CR

娴佺▼

鍗曠粏鑳炴墿澧炵殑鏁堟灉

娴佸紡鍒嗛€夌檶鐥囩粏鑳為噰鐢≒icoPLEX WGA kit杩涜鎵╁锛屼互鍚屼竴姣旂巼鎵╁锛屽苟涓旂粨鏋滀竴鑷达紝鍙鏈熺殑浜ч噺銆傜豢鑹蹭负涓嶅惈缁嗚優鐨勬牱鏈紝鏄剧ず鍑洪潪甯镐綆鐨勮儗鏅€笺€侟/p>

鍙噸澶嶇殑CNV缁撴灉

鍗曡儦鍙剁粏鑳炴椿妫€鏍锋湰浣跨敤PicoPLEX WGA kit 杩涜鎵╁锛屾爣璁帮紝鏉備氦鍒癇lueGnome鐨?4Sure鑺墖涓娿€傚浘涓竻鏅扮殑鎸囧嚭浜咰NVs銆?011骞达紝ESHRE杩涜涓村簥璇曢獙纭畾浜哖icoPLEX鎶€鏈繘琛屾牳鍨嬪垎鏋愮殑鍑嗙‘鎬с€侟/p>

浣嶇偣鐗瑰紓鎬-PCR鐢ㄤ簬瀹氶噺48浣嶇偣鍦ㄧ嫭绔嬬殑鍗曠粏鑳炴枃搴撲腑銆備袱涓崟鐙殑鏍锋湰閲囩敤PicoPLEX WGA kit杩涜鎵╁锛屼笅鍥炬瘮杈冧簡涓や釜缁撴灉锛孏enomePLEX鍜孏enomiPhi浣跨敤10pgDNA銆傝秴杩?0%鐨勭粨鏋滃彲楠岃瘉锛岃〃鐜板嚭浜嗛潪甯搁珮鐨勯噸澶嶆€у拰鍙潬鎬с€備粠宸﹀埌鏈夊垎鍒负锛歅icoPLEX銆丟enomePLEX鍜孏enomiPhi銆侟/p>

鍌ㄥ瓨娓╁害锛?20掳C

淇濊川鏈燂細12涓湀

寤哄簱璧峰閲忥細

• 鍔ㄧ墿缁嗚優锛?-10涓紱e.g. 鑳氬彾缁嗚優锛屾瀬浣擄紝婊嬪吇灞傜粏鑳烇紝缇婃按缁嗚優锛孋TCs锛屽煿鍏荤粏鑳炪€傦級

• 1000-10000涓粏鑿岀粏鑳濣/p>