鏋滆潎琛屼负鐩戞祴绯荤粺

Drosophila Activity Monitor (DAM) system鑳借骞垮ぇ鐨勭敓鐗╃瀛﹀鐨勭洃娴嬫灉铦囧拰绫讳技澶у皬鐨勬槅铏湪杩愬姩鍜岀窘鍖栨柟闈㈢殑琛屼负瑙勫緥锛屾绯荤粺宸茶搴旂敤浜庣獊鍙樺紓绉嶃€佺幆澧冪洃娴嬪拰瀵瑰寲瀛﹁嵂鍝佺殑鏁忔劅鎬э紝涔熷彲鐢ㄦ潵灞曠ず缇ゅ眳鐢熸椿涓浉浜掗棿鐨勪綔鐢ㄣ€侟/p>

鏋滆潎琚崟鐙斁缃湪鐜荤拑鐡剁毧涓紝閫氳繃绾㈠鍏夋潫鏉ユ帰娴嬪拰璁＄畻瀹冧滑鐨勮繍鍔ㄨ涓猴紝鍦ㄤ竴瀹氱殑鍛ㄦ湡鍐咃紝杩欎簺绱鐨勮涓烘暟鎹皢浠庣洃瑙嗗櫒涓婁紶鍒拌绠楁満涓瓨鍌ㄥ拰鍒嗘瀽.

*杩愬姩鐩戣鍣細鐢?2涓唴寰勪负 5mm,7mm鎴栬€?0mm鐨勭瀛愶紝鍒嗗埆鐩戞祴32涓灉铦囨牱鏈殑琛屼负瑙勫緥銆備竴涓孩澶栧厜鏉熺┛杩囨妸绠″瓙鍒嗘垚涓ゅ崐锛屽綋鏋滆潎鍦ㄧ瀛愪腑鏉ュ洖璧板姩锛屽畠浠皢鍒囧壊绾㈠鍏夛紝杩欎簺杩愬姩鏁版嵁鍒欒璁板綍涓嬫潵銆侟/p>

*缇藉寲鐩戣鍣細灏嗘垚鐔熺殑鏋滆潎铏嵉绮樿创鍦ㄤ竴涓湐鏂欏渾鐩樺簳涓嬶紝鐢ㄤ竴涓唴寰?00mm鐨勭幓鐠冩紡鏂楁潵鏀堕泦铏嵉瀛靛寲鍑烘潵鐨勬灉铦囥€備竴涓孩澶栫洃娴嬬幆瀹夎鍦ㄦ紡鏂椾笅鏂圭殑棰堝彛澶勶紝鐢ㄦ潵鐩戞祴璁＄畻姣忎竴涓€氳繃鐨勬灉铦囷紝涓€涓憞鏅冪殑鏈烘瑁呯疆鍙互瀹氭湡鐨勬憞鏅冩湐鏂欏渾鐩橈紝浣垮緱瀛靛寲鍑烘潵鐨勬灉铦囪兘澶熷畬鍏ㄤ粠鍦嗙洏鎺夎惤鍒扮幓鐠冩紡鏂椾腑銆侟/p>

*缇よ仛鐩戣鍣細鐢ㄤ竴涓唴寰?5mm鐨勭幓鐠冩垨鑰呮湐鏂欏皬鐡跺瓙锛岃鍏ュ涓灉铦囷紝鏍规嵁鐡跺瓙鐨勯暱搴︽斁缃嚑涓孩澶栫洃娴嬬幆锛岃褰曟灉铦囪繍鍔ㄦ€绘暟銆侟/p>

*鐜鐩戞祴鍣細娴嬮噺鍩瑰吇鍣ㄧ毧鍐呯殑鍏夌殑寮哄害锛屾俯搴﹀拰鐩稿叧鐨勬箍搴?/p>

*鐢垫簮锛氭彁渚涗竴涓洿娴佺數婧愮敤浜庢暣涓郴缁燂紝閫氳繃涓€涓數 璇濈嚎缃戠粶涓€鏍风殑骞宠鎺ュ彛锛岃繖涓數婧愬彲浠ユ彁渚涚粰澶氳揪120涓洃瑙嗗櫒鍚屾椂浣跨敤

*璁板綍杞欢锛氭彁渚涗竴涓瓨鍌ㄦ。妗堜粠涓€涓垨澶氫釜鐩戣鍣ㄥ悓鏃惰褰曡涓烘暟鎹€備竴涓疄楠屽彲杩炵画璁板綍鏁板皬鏃躲€佹暟澶╃敋鑷虫槸鍑犱釜鏄熸湡銆侟/p>

The Drosophila Activity Monitoring System enables a biologist to accurately characterize the locomotor and eclosion behavior rhythms in drosophila fruit flies and similarly-sized insects. The system has been used to screen for mutants, measure environmental and chemical sensitivities, and characterize social interaction, all based on the patterns of physical movement which the system observes and quantifies over time.

The individual flies are placed into glass chambers, where their confined motion may be detected and counted by infrared light beams in one or more activity monitoring units. At periodic intervals, these accumulated activity counts are uploaded from the monitors to a host Macintosh or Windows computer for storage and analysis.

The Locomotor Monitor uses 32 tubes of 5, 7, or 10 mm diameter, and measures the activity rhythms of 32 individual flies. An infrared beam bisects each tube, and detects motion as the flies walk back and forth from end to end.

The Eclosion Monitor uses a 100 mm dia. glass funnel to collect emerging flies as they fall from their pupae cases on the underside of a plastic disk. An infrared ring detector at the funnel neck counts each as it passes by, and a mechanical tapper dislodges periodically those which remain adhered to the disk or funnel walls.

The Population Monitor uses a 25 mm dia. glass or plastic vial to contain a multiplicity of flies, and measures their aggregate movement with ring detectors spaced along the vial length.

The Environment Monitor measures the light intensity, temperature, and relative humidity within an incubator chamber, and provides a continuous archival record of these conditions over the duration of an experiment.

The Power Supply Unit supplies DC power to an entire system of up to 120 monitors using a parallel network of residence-type telephone cables. An optional Light Controller allows up to 6 incubator lights to be independently cycled on and off under computer control.

The DAMSystem Collection Software provides archival storage of the collected activity data from a number of monitors simultaneously, and allows for continuous operation over the hours, days, or weeks of an experimental run.

浜у搧浠嬬粛

DAM5M鏋滆潎娲诲姩鐩戣鍣ㄥ彲鍚屾椂娴嬮噺32鍙灉铦囩殑鑷彂娲诲姩锛屽彲鐢ㄤ簬鏋滆潎椋熺墿锛坒ood锛夈€佹皵鍛筹紙odor锛夈€佹柟鍚戯紙orientation锛夈€佺伅鍏夛紙light锛夌瓑浣嶇疆鍋忕埍锛坧osition preference锛夊疄楠岋紝姣忎釜璇曠鐩村緞涓?mm,褰撴灉铦囨潵鍥為琛屾椂锛屽彧瑕佷腑鏂瘯绠?/4绾㈠鍏夋潫锛屽氨灏嗘湁涓€涓褰曚俊鍙凤紝姣忎釜璇曠鏈?涓嫭绔嬬殑绾㈠鍏夋潫锛屼笉浣嗗彲浠ヨ褰曢仐婕忕殑鏁帮紝鑰屼笖绾㈠鍒扮孩澶栫殑绉诲姩鏉滅粷浜嗗師鍦拌鏁帮紝杩樺彲璁板綍鍦ㄦ瘡涓孩澶栧厜鏉熺殑鍋滅暀鏃堕棿锛坉well time锛夛紝鍐呯疆瀹夊叏鐨勮瘯绠″浐瀹氬す锛屾槗浜庣Щ鍔ㄥ拰鎿嶄綔锛屽叏寮€鏀捐璁★紝璇曠鐏厜鍧囧寑娓楅€忥紝鏃犻槾褰便€侟/p>

鐗圭偣

32涓瘯绠＄敤浜?2鍙灉铦嘃/p>

鐩村緞5mm璇曠鐢ㄤ簬榛戣吂鏋滆潎锛坉rosophila Melanogaster锛堻/p>

姣忎釜璇曠鏈?涓嫭绔嬬殑绾㈠鍏夋潫

鐢熸垚璁℃暟锛圕ounts锛夈€佺Щ鍔紙Moves锛夈€佸仠鐣欐椂闂达紙Dwell锛夈€佷紤鎭紙Rest锛夊拰姣忎釜鏋滆潎鐨勪綅缃暟鎹?/p>

鍐呯疆绠″す鐢ㄤ簬蹇€熷姞杞藉拰瀹夊叏澶勭悊璇曠

鍙姌鍙犵揣鍑戠殑瀛樺偍鍜岃浆杩愯瘯绠狘/p>

鎿嶄綔涓€鑷寸殑鏄庢殫鐏厜

绮惧瘑鐨勭閬撳竷灞€

鐢垫簮锛岃繛鎺ョ嚎鍜屾暟鎹噰闆嗚蒋浠迭/p>

鍏煎DAM2,LAM25锛孌EnM鍜屾湰鍏徃鍏朵粬鐩戞帶鍗曞厓

浜у搧瑙勬牸

璇曠鐩村緞锛?mm

璇曠闀垮害锛?5mm鎴栬緝澶х殑

灏哄锛 33.0 x 8.3 x 2.6 cm

璐ㄩ噺锛?.26 kg 锛堜笉鍚瘯绠★級

鐩搁偦绾㈠闂磋窛锛?.1mm

杩炴帴锛 4 wire, 6 position, RJ-11鐢佃瘽绾挎帴澶滁/p>

9 v鐩存祦鐢垫簮杈撳叆鍜屾暟鎹紶杈掽/p>

鏁版嵁閲囬泦杞欢锛欴AMSystem3锛屾搷浣滃钩鍙帮細Windows PC 鎴 Apple Macintosh

鏁版嵁绫诲瀷锛氱Щ鍔紙Moves锛夈€佽鏁帮紙Counts锛夈€佸仠鐣欐椂闂达紙Dwell锛夈€佷綅缃紙Position锛?銆佷紤鎭紙Rest锛堻/p>

鏁版嵁鏂囦欢鏍煎紡锛?2鍒 DAMSystem鏂囨湰
鎿嶄綔鐜锛氭甯稿疄楠屽锛屼笉缁撻湶

The Drosophila Activity Monitor measures the activity rhythms of 32 individual flies, each in its own glass tube. In a typical experiment, a food mixture is placed into one end of the empty tube, and sealed on the outside with a wax coating. The fly is then inserted into the tube, followed by a cotton plug or ventilated plastic cap on the other end. The tube is snapped into the plastic clips of the monitor case, and if the tube is horizontal, an active fly will walk back and forth from one end to the other. Each time that it does so, it breaks an infrared light beam which bisects the tube, perpendicular to its axis. This event is detected by the onboard electronics, and added to the count which is maintained for each channel. At periodic intervals, ranging from 1 second to 1 hour, the DAMSystem software in the host computer acquires from the monitor its set of 32 count totals, and saves them. This archival record of the activity of each fly over the duration of the experiment may be plotted or analyzed, and used, for example, to measure the period and phase of the internal circadian clock of each of the 32 flies. Such measurements have been instrumental to the scientific study of fly behavior, and its genetic basis - the circadian clock being the cornerstone.

Specifications

? Dimensions: 13.50 x 6.82 x 1.03鈥?nbsp; (343 x 173 x 26 mm)

? Mass: 1.2 kg

? Glass Tubes: Corning Pyrex standard wall tubing, according to model: DAM 5 = 5 mm diameter DAM10 = 10 mm diameter

? Maximum Tube Length: 3.00鈥 (76 mm)

? Interconnect: 4 wire, 6 position, RJ-11 modular telephone line jack to DAMSystem network for DC power input and data transmission

? Case material: PVC/ABS plastic

Features

?32 independent activity channels per monitor.

?Up to 120 monitors per system provide over 3000 simultaneous monitoring channels.

?Models available for various tube diameters: DAM 5 5 mm tube (for D. melanogaster) DAM10 10 mm tube

?Built-in clips hold tubes in place during handling.

?Recessed tube mounts allow monitors to be safely stacked with tubes installed.

?Telephone-type wiring jack allows easy connection to DAMSystem wiring network.

?Consistent operation in ambient light levels from bright laboratory to total darkness.

?Field-proven design over years of continuous use.

?Optional accessories: Pyrex Glass Tubes 5 mm dia x 65 mm length 10 mm dia x 70 mm length

The DAM2 Drosophila Activity Monitor measures the locomotor activity of 32 individual flies, each in a separate tube. As a fly walks back and forth within its tube, it interrupts an infrared beam that crosses the tube at its midpoint, and this interruption, detected by the onboard electronics, is added to the tube鈥檚 activity count as a measure of fly activity. In a typical experiment, an agar/sucrose food mixture is placed into one end of each tube, followed by the fly, and followed by a cotton plug in the open end. The 32 tubes are inserted into holes in the monitor case, and centered. Over the course of the experiment, which may last for days or weeks, the counting circuits continuously monitor the activity in all 32 tubes, and at periodic intervals, upload their count totals to the host computer for storage and later analysis. This daily record provides a good measure of both the intensity of locomotor activity, and the relative periods of rest. The DAM2 is fabricated from a transparent polycarbonate plastic, allowing light penetration to all points along the tube. It is compact, light in weight, and when used within an incubator, allows plentiful air circulation around the tubes. The through-hole design accommodates tubes of arbitrary length. An on/off visible light sensor is built into each unit to provide a simple record of the ambient light state over time. This record accompanies the activity count data as it is acquired and stored, and is useful in analyzing circadian rhythms and adaptations of the flies to external light stimuli. An optional gas distribution manifold is available to facilitate the controlled exchange of air within the tubes, as would be needed for hypoxia studies.

Features

?32 independent activity channels per monitor.

?Up to 120 monitors per system provide over 3000 simultaneous monitoring channels.

?Compact design allows efficient use of incubator shelf space.

?Standard 5mm tube size (model DAM2) for studies of drosophila melanogaster.

?7mm tube size (model DAM2-7) accommodates d. virilis and larger species.

?Ambient light sensor provides on/off record of entrainment cycles and stimulation pulses, as well as inadvertent light exposure.

?Consistent operation in ambient light levels from bright laboratory to total darkness.

?Optional gas distribution manifold allows controlled air exchange within the tubes.

?Available tubes (diameter x length):

PGT5x65 = 5 x 65 mm Pyrex Glass

PGT7x65 = 7 x 65 mm Pyrex Glass

PPT5x65 = 5 x 65 mm Polycarbonate Plastic Plastic caps are available to seal the food in place of the traditional hot wax dip.

Specifications

?Dimensions: 127 x 48 x 87 mm LWH (5.0 x 1.9 x 3.4鈥?

?Mass: 170g

?Tube diameter: DAM 2 = 5 mm DAM 2-7 = 7 mm

?Ambient Light Sensor threshold: 10 lux nominal, phoic response curve

?Interconnect: 4 wire, 6 position, RJ-11 modular telephone line jack to DAMSystem network for DC power input and data transmission

?Case material: Clear polycarbonate plastic