ACHN人肾腺癌细胞(2019年新引进)
半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-5063 |
标识符
Identifier |
CSTR:19375.09.3101HUMSCSP5063 |
描述
Description |
细胞株始建于1979年11月，源于一名22岁的白种人男性恶性胸腔积液，他患有广泛转移的肾腺癌。该细胞的生长受到人干扰素抑制，应用于人干扰素或干扰素诱导物的抗增殖研究。每个批次均通过本库支原体检测，结果为阴性。经本库STR检测无误。STR鉴定结果为：Amelogenin: X；CSF1PO: 11；D13S317: 12；D16S539: 12,13；D5S818: 12；D7S820: 9,11；THO1:8；TPOX: 8,11；vWA: 16,17。 |
动物种别
Organism |
人；白人 |
性别
Gender |
男性，22岁 |
组织来源
Tissue and Cell Type |
肾脏；来源于转移部位：胸腔积液 |
形态
Morphology |
上皮样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
人肾腺癌细胞ACHN完全培养液配方(100 ml)：
MEM (Gibco, 110900081)87 ml
Glutamax (Gibco, 35050-061) 1 ml
Sodium Pyruvate (Gibco, 11360-070) 1 ml
Non-essential Amino Acids, 100 (Invitrogen, 11140050) 1 ml
FBS (Gibco)10 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
生物安全等级：BSL-1
参考传代比例：1:2-1:3
参考传代周期：3-5天
参考换液频率：每周3次
冻存液配方：完全培养液95%，DMSO 5%
【注意事项】
该细胞生长受到人类干扰素抑制 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells The ACHN cell line was initiated in November, 1979 from the malignant pleural effusion of a 22-year-old Caucasian male with widely metastatic renal adenocarcinoma (autopsy confirmed). Cells were seeded directly to culture flasks in Eagle's MEM with 10% FBS, then maintained and passaged 150 days in flasks. Cells were then inoculated subcutaneously into nude mice. After 4 weeks, palpable, locally invasive tumors were noted. Both the original cells (ACHN) and those recovered from nude mouse tumors were growth-inhibited by human interferons. Kochevar J. Blockage of autonomous growth of ACHN cells by anti-renal cell carcinoma monoclonal antibody 5F4. Cancer Res. 20: 2968-2972, 1990. PubMed: 2334900 |
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