琛€娴嗘父绂籇NA鎻愬彇璇曞墏鐩掞紙纾佺彔娉曪級BunnyMag?琛€娴嗘父绂籇NA鎻愬彇璇曞墏鐩掞紙纾佺彔娉曪級閲囩敤鍏锋湁鐙壒鍒嗙浣滅敤鐨勭鐝犲拰鐙壒鐨勭紦鍐叉恫绯荤粺锛屽彲浠?.5-4 mL鏂伴矞鎴栧喎鍐荤殑琛€娴嗐€佽娓呫€佽兏姘淬€佽吂姘村強鑴戣剨娑茬瓑鏃犵粏鑳炰綋娑蹭腑鎻愬彇楂樿川閲廲fDNA銆侟img alt="" data-cke-saved-src="//www.vuestras.com/data/daily/2022/03/01/152530_871996_pro_auto_1049291.png" src="//www.vuestras.com/data/daily/2022/03/01/152530_871996_pro_auto_1049291.png"/>浜у搧鎻忚堪琛€娴嗘父绂籇NA锛坈ell-free DNA, cfDNA锛夋槸涓€绉嶇敱缁嗚優閲婃斁鍒拌娑蹭腑鐨勬父绂籇NA鐗囨锛屽畠鍙互鎼哄甫鏉ヨ嚜鑲跨槫銆佺梾鍘熶互鍙婅儙鍎跨殑鐢熺墿瀛︿俊鎭€傞€氳繃鍒嗘瀽cfDNA鎻愪緵鐨勫叧閿爣璁扮墿鑳藉杈惧埌妫€娴嬨€佽瘖鏂苟鐩戞祴鐤剧梾杩涘睍鐨勭洰鐨勩€傜洰鍓嶏紝璇ユ妧鏈凡骞挎硾搴旂敤浜庤偪鐦ょ獊鍙樻娴嬨€侀澏鍚戠敤鑽寚瀵煎拰棰勫悗銆佺梾鍘熸娴嬪強鏃犲垱浜у墠绛涙煡绛夐鍩熴€侟br/> 浼樿川鐨勬牳閰告槸鍩哄洜缁勫鐮旂┒鐨勪繚闅溿€傚厰鐗欑鎶€閲囩敤瓒呴『纾佹€х鐝犵函鍖栨妧鏈紝涓虹敤鎴锋彁渚涗簡楂樼伒鏁忕殑鏍搁吀鎻愬彇鏂规锛岃交鏉惧疄鐜颁綆娴撳害鐨勬牳閰告彁鍙栥€傛湰浜у搧缁忚繎涓囦緥鐪熷疄涓村簥鏍锋湰楠岃瘉锛屽彲浠庢柊椴滄垨鍐峰喕鐨勮娴嗐€佽娓呫€佽兏姘淬€佽吂姘村強鑴戣剨娑茬瓑鏃犵粏鑳炰綋娑蹭腑鎻愬彇楂樿川閲廲fDNA锛屼互渚涚鐮斿強涓村簥浣撳璇婃柇浣跨敤銆侟br/>浜у搧浼樺娍楂樻晥鐨勬彁鍙栨晥鐜囷細鎻愬彇浜ч噺楂橈紝绾害濂斤紝鏈夋晥鍘婚櫎鏉傝川鍜屼笅娓窹CR鎶戝埗鍥犲瓙锛汓br/>鐏垫椿鐨勬牱鏈姞鍏ワ細杞绘澗瀹炵幇浠?.5 mL鍒? mL鏍锋湰涓彁鍙朿fDNA锛屾礂鑴变綋绉綆鑷?0 渭L锛汓br/>澶氬厓鐨勫簲鐢ㄥ満鏅細缁忕湡瀹炰复搴婃牱鏈獙璇侊紝涓撲负鑲跨槫銆佹劅鏌撳強浜у墠璇婃柇娑蹭綋娲绘璁捐锛汓br/>骞挎硾鐨勮嚜鍔ㄥ寲骞冲彴锛欬/strong>鍏煎鎵嬪姩鎻愬彇鍙婅嚜鍔ㄦ彁鍙栫▼搴忥紝閫傞厤Tecan銆丠amilton銆丳E绛夋牳閰告彁鍙栧伐浣滃钩鍙般€侟br/>瀹為獙妗堜緥楂樻晥鍥炴敹涓嶅悓鏍搁吀鍚噺鐨勮娴哻fDNA鍚戠湡瀹炶娴嗚儗鏅腑Spike-in涓嶅悓閲忕殑鏍搁吀锛屽垎鍒ā鎷?绉嶆搴︾殑浣庢牳閰稿強楂樻牳閰稿惈閲忔牱鏈紝浣跨敤3绉嶈瘯鍓傜洅鍚屾杩涜鏍搁吀鎻愬彇锛孊unnyMag? cfDNA鎻愬彇璇曞墏鐩掑彲浠庝笉鍚屾牳閰稿惈閲忕殑鏍锋湰涓珮鏁堝洖鏀禼fDNA鐗囨锛屾牳閰镐骇閲忓強閲嶇幇鎬т紭浜庣珵鍝佸叕鍙稿悓绫讳骇鍝併€侟br/>涓嶅悓浣撶Н琛€娴嗘牱鏈腑cfDNA鍥炴敹鏁堢巼鍛堣壇濂界嚎鎬у叧绯狐/strong>浣跨敤BunnyMag? cfDNA鎻愬彇璇曞墏鐩掑垎鍒0.5銆?銆?銆? mL琛€娴嗘牱鏈繘琛屾彁鍙栵紝cfDNA浜ч噺涓庤娴嗘牱鏈姇鍏ヤ綋绉憟鑹ソ鐨勭嚎鎬у叧绯伙紝缁廇gilent 2100 DNA妫€娴嬶紝涓嶅悓浣撶Н鏍锋湰鎻愬彇cfDNA鐨勫嘲褰㈠浘瀹屽叏涓€鑷淬€侟br/>cfDNA鑽у厜瀹氶噺PCR Ct鍊间笌绔炲搧涓€鑷存€ч珮浣跨敤涓嶅悓鍝佺墝cfDNA鎻愬彇璇曞墏鐩掑2 mL琛€娴嗘牱鏈彁鍙栨牳閰稿悗杩涜鑽у厜瀹氶噺锛孊unnyMag? cfDNA鎻愬彇璇曞墏鐩掓晥鏋滀笌绔炲搧鍏徃鍚岀被浜у搧涓€鑷淬€浜у搧缁勫垎瀹為獙鎿嶄綔娴佺▼鍥近br/>鍌ㄥ瓨鏉′欢纾佺彔鎮诞娑插拰铔嬬櫧閰禟缃簬2-8鈩冧繚瀛橈紝鍏朵粬璇曞墏鍙娓╀繚瀛橈紝鏈夋晥鏈?2涓湀銆侟br/>