浜у搧灞炴€э細浜у搧浠呯敤浜庣鐮擖/strong>

浜у搧浼樼偣濡備笅锛欬br/> 1銆佸揩閫熺畝渚匡細鍙岃瘯鍓傦紝96瀛旀澘鐩存帴鎿嶄綔锛屽揩閫熴€佺畝渚裤€侟br/> 2銆佸彇鏍烽噺寰細闇€瑕佺殑鏍锋湰閲忔槸缇熻兒娉曠殑1/10銆侟br/> 3銆佺伒鏁忓害楂橈細妫€娴嬬嚎0.5U/ml锛屾槸缇熻兒娉?鍊嶏紝閭昏嫰涓夐厷娉曠殑200鍊?nbsp;
4銆佺ǔ瀹氭€уソ锛氭壒鍐匔V=5.50%,鎵归棿CV=3.32%锛岃瘯鍓傜洅2锝?鈩冨瓨鏀?涓湀鏈夋晥銆?nbsp;
5銆佸啀鐜版€уソ锛氬彉寮傜郴鏁癈V=1.2%銆侟br/> 6銆佸洖鏀惰瘯楠岋細 X =102.3%銆侟br/> 7銆佸彈澶栫晫褰卞搷鍥犵礌灏忥細骞叉壈鍥犵礌灏戯紝閲嶅鎬у己銆備笖鐗瑰紓鎬у己锛屼笉鍙楃被SOD鐗╄川鐨勫共鎵?br/> 8銆佹祴璇曢潰骞匡細鍙祴鍔ㄧ墿琛€娑层€佺粍缁囥€佸悇绉嶄綋娑层€佺亴娴佹恫绛夈€佸悇绉嶅煿鍏荤粏鑳炪€佺粏鑿屻€佹鐗╃粍缁囥€佸悇绉嶆按浜т互鍙婂寲濡嗗搧銆佷繚鍋ュ搧绛夛紝鏁堟灉鍧囦匠銆?nbsp;
銆愭牱鏈墠澶勭悊姝ラ銆?nbsp;
鏍锋湰澶勭悊鍓嶉』鐭?br/> 澶勭悊浠讳綍鏍锋湰鏃?閮藉繀椤绘敞鎰廁
(1) 瀹為獙涓繀椤讳娇鐢ㄤ竴娆℃€у惛澶?鍦ㄥ惛鍙栦笉鍚岀殑璇曞墏鏃惰鏇存崲鍚稿ご(2)瀹為獙鍓嶉』妫€鏌ュ悇绉嶅疄楠屽櫒鍏锋槸鍚﹀共鍑€,蹇呰鏃跺瀹為獙鍣ㄥ叿杩 琛屾竻娲?閬垮厤姹℃煋骞叉壈瀹為獙缁撴灉銆?nbsp;
(a)缁勭粐鏍峰搧澶勭悊鏂规硶:
1銆 绉板彇5卤0.05g缁勭粐鏍峰搧浜?0ml绂诲績绠′腑,鍏堝姞鍏?ml宸茬█閲婂ソ鐨勬牱鍝佹彁鍙栨恫闇囪崱2min;鍐嶅姞鍏?0ml涔欒厛,鍏呭垎闇囪崱5min,
2銆 鍔犲叆2g涓€ф哀鍖栭摑;闇囪崱2min ,瀹ゆ俯4000r/min浠ヤ笂,绂诲績10min;;
3銆 鍙?ml涓婃竻娑蹭簬骞插噣绂诲績绠′腑,鍔犲叆5ml浜屾隘鐢茬兎闇囪崱3min ,瀹ゆ俯4000r/min浠ヤ笂,绂诲績10min;;
4銆 鍙栦笅灞傛竻婢堟恫浣撲簬鐜荤拑绠′腑,鍔犲叆20ul姘у寲鍓傛贩鍖€,鍦?6鈩冩潯浠朵笅姘皵鎴栫┖姘旀祦鍚硅嚦瀹屽叏骞茬嚗;
5銆 鍔犲叆1ml澶嶆憾娑?鍏呭垎婧惰В骞茬嚗娈嬬暀鐗┿€?nbsp;
6銆 鍙?00ul 涓婃竻娑蹭笌100ul 宸茬█閲婂ソ鐨勫婧舵恫鍏呭垎娣峰悎30s,
7銆 鍙?0ul杩涜鍒嗘瀽銆?nbsp; 鏍锋湰绋€閲婂€嶆暟: 1鍊?nbsp;
(b)姘存牱鍝佸鐞嗘柟娉旡
1銆佸彇50ul 涓婃竻娑蹭笌450ul 宸茬█閲婂ソ鐨勫婧舵恫鍏呭垎娣峰悎30s,
2銆佸彇50ul杩涜鍒嗘瀽銆?nbsp; 鏍锋湰绋€閲婂€嶆暟:10鍊?nbsp;
鎿嶄綔姝ラ:
瀹為獙寮€濮嬪墠锛屽悇璇曞墏鍧囧簲骞宠　鑷冲娓╋紙璇曞墏涓嶈兘鐩存帴鍦?7鈩冩憾瑙ｏ級锛涜瘯鍓傛垨鏍峰搧绋€閲婃椂锛屽潎闇€娣峰寑锛屾贩鍖€鏃跺敖閲忛伩鍏嶈捣娉°€傚疄楠屽墠搴旈娴嬫牱鍝佸惈閲忥紝濡傛牱鍝佹祿搴﹁繃楂樻椂锛屽簲瀵规牱鍝佽繘琛岀█閲婏紝浠ヤ娇绋€閲婂悗鐨勬牱鍝佺鍚堣瘯鍓傜洅鐨勬娴嬭寖鍥达紝璁＄畻鏃跺啀涔樹互鐩稿簲鐨勭█閲婂€嶆暟銆?. 鍔犳牱锛氬垎鍒绌虹櫧瀛斻€佹爣鍑嗗瓟銆佸緟娴嬫牱鍝佸瓟銆傜┖鐧藉瓟鍔犳牱鍝佺█閲婃恫100渭l锛屼綑瀛斿垎鍒姞鏍囧噯鍝佹垨寰呮祴鏍峰搧100渭l锛屾敞鎰忎笉瑕佹湁姘旀场锛屽姞鏍峰皢鏍峰搧鍔犱簬閰舵爣鏉垮瓟搴曢儴锛屽敖閲忎笉瑙﹀強瀛斿锛岃交杞绘檭鍔ㄦ贩鍖€锛岄叾鏍囨澘鍔犱笂鐩栨垨瑕嗚啘锛?7鈩冨弽搴?20鍒嗛挓銆備负淇濊瘉瀹為獙缁撴灉鏈夋晥鎬э紝姣忔瀹為獙璇蜂娇鐢ㄦ柊鐨勬爣鍑嗗搧婧舵恫銆?nbsp;
2. 寮冨幓娑蹭綋锛岀敥骞诧紝涓嶇敤娲楁钉銆傛瘡瀛斿姞妫€娴嬫憾娑睞宸ヤ綔娑 100渭l锛堝湪浣跨敤鍓嶄竴灏忔椂鍐呴厤鍒讹級锛岄叾鏍囨澘鍔犱笂瑕嗚啘, 37鈩冨弽搴?0鍒嗛挓銆?. 娓╄偛60鍒嗛挓鍚庯紝寮冨幓瀛斿唴娑蹭綋锛岀敥骞诧紝娲楁澘3娆★紝姣忔娴告场1-2鍒嗛挓锛屽ぇ绾?00渭l/姣忓瓟锛岀敥骞诧紙涔熷彲杞绘媿灏嗗瓟鍐呮恫浣撴媿骞诧級銆侟br/> 4. 姣忓瓟鍔犳娴嬫憾娑睟宸ヤ綔娑诧紙鍚屾娴婣宸ヤ綔娑诧級 100渭l锛岄叾鏍囨澘鍔犱笂瑕嗚啘37鈩冨弽搴?0鍒嗛挓銆?nbsp;
5. 娓╄偛60鍒嗛挓鍚庯紝寮冨幓瀛斿唴娑蹭綋锛岀敥骞诧紝娲楁澘5娆★紝姣忔娴告场1-2鍒嗛挓锛?50渭l/姣忓瓟锛岀敥骞诧紙涔熷彲杞绘媿灏嗗瓟鍐呮恫浣撴媿骞诧級銆?nbsp;
6. 渚濆簭姣忓瓟鍔犲簳鐗╂憾娑?0渭l锛岄叾鏍囨澘鍔犱笂瑕嗚啘37鈩冮伩鍏夋樉鑹诧紙30鍒嗛挓鍐咃紝姝ゆ椂鑲夌溂鍙鏍囧噯鍝佺殑鍓?-4瀛旀湁鏄庢樉鐨勬搴﹀叞鑹诧紝鍚?-4瀛旀搴︿笉鏄庢樉锛屽嵆鍙粓姝級銆
7. 渚濆簭姣忓瓟鍔犵粓姝㈡憾娑?0渭l锛岀粓姝㈠弽搴旓紝姝ゆ椂钃濊壊绔嬭浆榛勮壊銆傜粓姝㈡恫鐨勫姞鍏ラ『搴忓簲灏介噺涓庡簳鐗╂恫鐨勫姞鍏ラ『搴忕浉鍚屻€備负浜嗕繚璇佸疄楠岀粨鏋滅殑鍑嗙‘鎬э紝搴曠墿鍙嶅簲鏃堕棿鍒板悗搴斿敖蹇姞鍏ョ粓姝㈡恫銆?nbsp;
8. 鐢ㄩ叾鑱斾华鍦?50nm娉㈤暱渚濆簭娴嬮噺鍚勫瓟鐨勫厜瀵嗗害锛圤D鍊硷級銆傚湪鍔犵粓姝㈡恫鍚庣珛鍗宠繘琛屾娴嬨€?nbsp;
琛€绠″唴鐨粏鑳炲畬鍏ㄥ煿鍏诲熀 100mL澶嶅纰崇撼绫崇 40-60nm(鐩村緞),5-15渭m(闀垮害)璐ㄩ噺瑙勬牸锛?gt;97%(TPO Method)Carbon Nanotube Multi-walled 40-60nm(diam.), 5-15渭m(length)

D341Med缁嗚優锛岄珦姣嶇粏鑳炵槫缁嗚優 钘ら粍寰悆鑿?鑵鹃粍鍏彔鐞冭弻 鐚偩缁嗚優;CRFK澶嶅纰崇撼绫崇 40-60nm(鐩村緞),1-2渭m(闀垮害)璐ㄩ噺瑙勬牸锛?gt;95%(TPO Method)Carbon Nanotube Multi-walled 40-60nm(diam.), 1-2渭m(length)

CCL3 Protein Mouse 閲嶇粍灏忛紶 CCL3 / Mip1a 铔嬬櫧澶嶅纰崇撼绫崇 60-100nm(鐩村緞),5-15渭m(闀垮害)璐ㄩ噺瑙勬牸锛?gt;95%(TPO Method)Carbon Nanotube Multi-walled 60-100nm(diam.), 5-15渭m(length)

NIH/3T3(灏忛紶鑳氳儙缁嗚優) 5脳106cells/鐡睹? EVI2A 缁嗚優瑁傝В娑 (闃虫€у鐓? 3,5-浜岃媱姘у熀鑻勬捍(>98.0%(GC)(T))璐ㄩ噺瑙勬牸锛?gt;98.0%(GC)(T)3,5-Dibenzyloxybenzyl Bromide

灏忛紶鑹茬礌鐦ょ槫鏍?B16澶嶅纰崇撼绫崇 10-20nm(鐩村緞),5-15渭m(闀垮害)璐ㄩ噺瑙勬牸锛?gt;97%(TPO Method)Carbon Nanotube Multi-walled 10-20nm(diam.), 5-15渭m(length)
浜虹櫧浠嬬礌1尾鍓嶄綋(pro-IL1尾)閰惰仈鍏嶇柅鍚搁檮娴嬪畾璇曞墏鐩 鍒О:pro leukocytic pyrogen,pro leukocytic endogenous mediator,pro mononuclear cell factor,pro lymphocyte activating factor,pro IL1B,pro IL1F2,pro Catabolin Human pro-IL1尾(pro interleukin 1 beta ) ELISA Kit 鐢ㄩ€ 璇ヨ瘯鍓傜洅鐢ㄤ簬浣撳瀹氶噺妫€娴婬uman 琛€娓咃紝琛€娴嗘垨鍏朵粬鐢熺墿浣撴恫涓ぉ鐒跺強閮ㄥ垎閲嶇粍pro-IL1尾娴撳害

浜衡厾鍨嬭兌鍘熶氦鑱旂晶鍩虹鑲?CTX鈪?閰惰仈鍏嶇柅鍚搁檮娴嬪畾璇曞墏鐩 鍒О:CTX-I, CTX1, CTXI Human CTX鈪?Cross Linked C-telopeptide of Type 鈪 Collagen) ELISA Kit 鐢ㄩ€ 璇ヨ瘯鍓傜洅鐢ㄤ簬浣撳瀹氶噺妫€娴婬uman 琛€娓咃紝琛€娴嗘垨鍏朵粬鐢熺墿浣撴恫涓ぉ鐒跺強閮ㄥ垎閲嶇粍CTX鈪犳祿搴?/p>

浜哄熀璐ㄩ噾灞炶泲鐧介叾7(MMP-7)閰惰仈鍏嶇柅鍚搁檮娴嬪畾璇曞墏鐩 鍒О:MMP7, MPSL1, PUMP-1, Matrilysin Human MMP-7(Matrix Metalloproteinase 7) ELISA Kit 鐢ㄩ€ 璇ヨ瘯鍓傜洅鐢ㄤ簬浣撳瀹氶噺妫€娴婬uman 琛€娓咃紝琛€娴嗘垨鍏朵粬鐢熺墿浣撴恫涓ぉ鐒跺強閮ㄥ垎閲嶇粍MMP-7娴撳害
NADP+-灞辨ⅷ閱囪劚姘㈤叾(NADP+-SDH)璇曞墏鐩掑師鐞咟/strong>浜虹矑缁嗚優瓒嬪寲铔嬬櫧2(GCP-2)閰惰仈鍏嶇柅鍚搁檮娴嬪畾璇曞墏鐩 鍒О:CXCL6, GCP2, SCYB6, CKA-3 Human GCP-2(Granulocyte Chemotactic Protein 2) ELISA Kit 鐢ㄩ€ 璇ヨ瘯鍓傜洅鐢ㄤ簬浣撳瀹氶噺妫€娴婬uman 琛€娓咃紝琛€娴嗘垨鍏朵粬鐢熺墿浣撴恫涓ぉ鐒跺強閮ㄥ垎閲嶇粍GCP-2娴撳害
浠櫒锛?nbsp;
1銆佸垎鍏夊厜搴﹁/閰舵爣浠?鍗婅嚜鍔ㄧ敓鍖栧垎鏋愪华锛堟瘮鑹叉祴瀹氭尝闀夸负550nm锛?nbsp;
2銆佹亽娓╂按娴寸鎴栨皵娴寸 锛堝鑲叉俯搴︿负37鈩冿級
3銆佸彴寮忕蹇冩満
4銆佹缉娑℃贩鍖€鍣?/p>