Catalog # Product Description |
ICP0810 This antibody was raised from rabbits that were immunized with NG,NG-dimethyl arginine-KLH conjugates and specifically isolated and affinity purified using the NG,NG-dimethyl arginine antigen. The affinity purified methyl arginine antibody is useful for detection, isolation and localization of proteins with methylated arginine (asymmetric dimethylation) residues. |
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Western blot of human MMRU cells with ICP0810 (A,B) plus competitive inhibition with free dimethyl arginine (C,D)
Antigen Structure
IF Staining of MMRU cells
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Species
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Rabbit
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Formulation
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250μg/mL antibody stored in PBS, 50% glycerol
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Immunogen
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N
G
,N
G
-dimethyl arginine-KLH conjugates
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Purification
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Antigen-specific immunoaffinity purification with N
G
,N
G
-dimethyl arginine and adsorbed with N
G
-monomethyl arginine
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Specificity
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The antibody recognizes proteins with dimethylated arginine residues. It does not cross-react with acetylated proteins or monomethylated proteins.
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Species Tested
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Human, mouse and pig
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Applications
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ELISA (1:2000); WB (1:1000); IP (5 μg/mg protein sample); IF (5 μg/mL); IHC (5 μg/mL)
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Scientific Description
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Arginine methylation is a very common post-translational modification that occurs predominantly in the nucleus. The main pool of proteins that are modified possess RNA binding properties. More broadly, arginine methylation has been implicated in a variety of cellular processes, including protein trafficking, signal transduction and transcriptional regulation.
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Storage & Stability
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Product is stable for several weeks at 4°C. For extended storage, store product at –20oC. Expiration date is one year from date of shipping if properly stored.
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Product Specific References
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1.J. Proteome Res.2013.12(9): 3884-3899.doi:10.1021/pr400556c
2.Mol. Microbiol.2007.65(3): 590-606,doi:10.1111/j.1365-2958.2007.05831.x
3.Proteomics. 2016.16(3): 465-476.doi: 10.1002/pmic.201400564
4.Proteomics.2013.13(7): 1059-1064.doi: 10.1002/pmic.201200477
5.Proteomics.2015.15(18): 3209-3218.doi: 10.1002/pmic.201500075
6.J. Neurosci. 2015.35(37): 12890-12902.doi: 10.1523/JNEUROSCI.0246-15.2015 |
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