鐚槦鐥呮瘨(FPV)鏍搁吀妫€娴嬭瘯鍓傜洅(鑽у厜PCR娉?

鍨嬪彿:	RD-D-0811
鍝佺墝:	缁翠集閼敓鐗?/td>
鍙傝€冧环鏍机	1000鍏?鐩扅/td>
鍖呰:	10T/鐩
浜よ揣鏈烔	1鍛?/td>
浜у湴:	骞垮窞

绱㈠彇璧勬枡鍙婃姤浠饵/span>

浜у搧鍚嶇О

Feline Panleukopenia Virus Detection Kit (Real-Time PCR Method)

浜у搧浠嬬粛

銆愪骇鍝佸悕绉般€慄/strong>

閫氱敤鍚嶇О锛氱尗鐦熺梾姣?FPV)鏍搁吀妫€娴嬭瘯鍓傜洅(鑽у厜PCR娉?

Name锛欶eline Panleukopenia Virus Detection Kit (Real-Time PCR Method)

銆愬寘瑁呰鏍笺€慄/strong>10T/鐩?nbsp;

銆愰鏈熺敤閫斻€慄/strong>

鐚硾鐧界粏鑳炲噺灏戠棁锛屼篃绉扮尗鐦熺儹锛屾槸鐢辩尗娉涚櫧缁嗚優鍑忓皯鐥囩梾姣掞紙Feline Panleuk -openia Virus, FPV锛夊紩璧风殑涓€绉嶉珮搴︽帴瑙︽€т紶鏌撶梾銆傝鐥呭湪鑷劧鏉′欢涓嬪彲鎰熸煋鐚銆佹担鐔婄鍜岄棘绉戠瓑鍔ㄧ墿锛屾劅鏌撴瘮杈冩櫘閬嶃€侳PV浼犳挱鎬у己锛屾劅鏌撳姩鐗╁彲閫氳繃绮究銆佸憰鍚愮墿銆侀蓟娑曘€佸斁娑层€佸翱娑叉帓鍑虹梾姣掍粠鑰屾薄鏌撻鍏枫€佺瀛愩€佸灚鐗╁拰鍛ㄥ洿鐜锛屽濞犳瘝鐚篃鍙瀭鐩翠紶鎾粰鑳庡効锛岃烦铓ゅ拰鍚歌鏄嗚櫕鍙垚涓轰紶鎾獟浠嬨€侳PV鑷寸梾鎬у己锛屽濞犳瘝鐚儙鍎跨暩褰㈣儙鐢氳嚦娴佷骇锛屾浜＄巼楂樹笖鏃犱换浣曞墠椹辩棁鐘迭sup>[1]銆侟/p>

鏈瘯鍓傜洅閫傜敤浜庢娴嬫硠娈栬厰妫夋嫮瀛愩€佺勃渚裤€佸叏琛€锛岀梾姝荤尗鐨勮偁閬撶瓑鏍锋湰涓殑鐚槦鐥呮瘨锛岀敤浜庣尗鐦熺梾姣掓劅鏌撶殑杈呭姪璇婃柇銆侟/p>

銆愭楠屽師鐞嗐€慄/strong>

鏈瘯鍓傜洅閲囩敤TaqMan鎺㈤拡娉曞疄鏃惰崸鍏塒CR鎶€鏈紝璁捐涓€瀵圭尗鐦熺梾姣掑熀鍥犵粍鐗瑰紓鎬у紩鐗╋紝缁撳悎涓€鏉＄壒寮傛€ф帰閽堬紝鐢ㄨ崸鍏塒CR鎶€鏈鐚槦鐥呮瘨鍩哄洜缁勭殑DNA杩涜浣撳鎵╁妫€娴嬶紝鐢ㄤ簬涓村簥涓婂鍙枒鎰熸煋鑰呯殑鐥呭師瀛﹁瘖鏂€侟/p>

銆愯瘯鍓傜粍鎴愩€慄/strong>

鍖呰瑙勬牸	25T/鐩扅/p>	50T/鐩扅/p>
FPV鍙嶅簲娑秉/p>	500渭L脳1绠狘/p>	500渭L脳2绠狘/p>
閰舵恫	25渭L脳1绠狘/p>	50渭L脳1绠狘/p>
FPV闃虫€ц川鎺у搧	50渭L 脳1绠狘/p>	50渭L 脳1绠狘/p>
闃存€ц川鎺у搧	250渭L 脳1绠狘/p>	250渭L 脳1绠狘/p>

璇存槑锛氫笉鍚屾壒鍙风殑璇曞墏鐩掔粍鍒嗕笉鍙氦浜掍娇鐢ㄣ€侟/p>

銆愬偍瀛樻潯浠跺強鏈夋晥鏈熴€慄/strong>-20鈩兟?鈩冿紝閬垮厜淇濆瓨銆佽繍杈撱€佸弽澶嶅喕铻嶆鏁颁笉瓒呰繃5娆★紝鏈夋晥鏈?2涓湀銆侟/p>

銆忺/strong>閫傜敤浠櫒銆慄/strong>ABI 銆佸畨鎹蜂鸡MX3000P/3005P銆丩ightCycler銆丅io-Rad銆丒ppendorf绛夌郴鍒楄崸鍏夊畾閲廝CR妫€娴嬩华銆侟/p>

銆愭爣鏈噰闆嗐€慄/strong>鎮ｇ梾鐚瓑鍔ㄧ墿锛氭硠娈栬厰妫夋嫮瀛愩€佺勃渚裤€佸叏琛€锛涚梾姝荤尗锛氳偁閬撳唴瀹圭墿

銆愪繚瀛樺拰杩愯緭銆慄/strong>涓婅堪鏍囨湰鐭湡鍐呭彲淇濆瓨浜?20鈩冿紝闀挎湡淇濆瓨鍙疆-70鈩冿紝浣嗕笉鑳借秴杩?涓湀锛屾爣鏈繍閫佸簲閲囩敤2~8鈩冨啺琚嬭繍杈擄紝涓ョ鍙嶅鍐昏瀺銆侟/p>

銆忺/strong>浣跨敤鏂规硶銆慄/strong>

1.鏍峰搧澶勭悊锛堟牱鏈鐞嗗尯锛堻/strong>

1.1鏍锋湰鍓嶅鐞咟/strong>

缁勭粐鏍峰搧锛氭瘡浠界粍缁囧垎鍒粠3涓笉鍚岀殑浣嶇疆绉板彇鏍峰搧绾?g锛屾墜鏈壀鍓娣峰寑鍚庡彇0.5g浜庣爺纾ㄥ櫒涓爺纾紝鍔犲叆1.5mL鐢熺悊鐩愭按鍚庣户缁爺纾紝寰呭寑娴嗗悗杞嚦1.5mL鐏弻绂诲績绠′腑锛?000rpm绂诲績2min锛屽彇涓婃竻娑?00渭L浜?.5mL鐏弻绂诲績绠′腑锛涘捊鎷瓙鏍峰搧鐩存帴鍙?00渭L浜?.5mL鐏弻绂诲績绠′腑銆侟/p>

1.2鏍搁吀鎻愬彇

鎺ㄨ崘閲囩敤鎴戝徃鐢熶骇鐨勬牳閰告彁鍙栨垨绾寲璇曞墏锛堢鐝犳硶鎴栫蹇冩煴娉曪級杩涜鏍搁吀鎻愬彇锛岃鎸夌収璇曞墏璇存槑涔﹁繘琛屾搷浣溿€侟/p>

2.璇曞墏閰嶅埗锛堣瘯鍓傚噯澶囧尯锛堻/strong>

鏍规嵁寰呮娴嬫牱鏈€绘暟锛岃鎵€闇€瑕佺殑PCR鍙嶅簲绠＄鏁颁负N(N=鏍锋湰鏁?1绠￠槾鎬у鐓?1绠￠槼鎬у鐓э紱鏍峰搧姣忔弧10浠斤紝澶氶厤鍒?浠?锛屾瘡娴嬭瘯鍙嶅簲浣撶郴閰嶅埗濡備笅琛細

璇曞墏	FPV鍙嶅簲娑秉/p>	閰舵恫
鐢ㄩ噺锛堟牱鏈暟涓篘锛堻/p>	20渭L	1渭L

灏嗘贩鍚堝ソ鐨勬祴璇曞弽搴旀恫鍒嗚鍒癙CR鍙嶅簲绠′腑锛?1uL/绠°€侟/p>

3.鍔犳牱锛堟牱鏈鐞嗗尯锛堻/strong>

灏嗘楠?鎻愬彇鐨勬牳閰搞€侀槼鎬ц川鎺у搧銆侀槾鎬ц川鎺у搧鍚勫彇4渭L锛屽垎鍒姞鍏ョ浉搴旂殑鍙嶅簲绠′腑锛岀洊濂界鐩栵紝娣峰寑锛岀煭鏆傜蹇冦€侟/p>

4.PCR鎵╁锛堟牳閰告墿澧炲尯锛堻/strong>

4.1 灏嗗緟妫€娴嬪弽搴旂缃簬鑽у厜瀹氶噺PCR浠弽搴旀Ы鍐咃紱

4.2 璁剧疆濂介€氶亾銆佹牱鍝佷俊鎭紝鍙嶅簲浣撶郴璁剧疆涓?5渭L锛汓/p>

鑽у厜閫氶亾閫夋嫨锛妫€娴嬮€氶亾锛圧eporter Dye锛塅AM锛娣伃閫氶亾锛圦uencher Dye锛塏ONE锛孉BI绯诲垪浠櫒璇峰嬁閫夋嫨ROX鍙傛瘮鑽у厜锛岄€夋嫨None鍗冲彲銆侟/p>

4.3 鎺ㄨ崘寰幆鍙傛暟璁剧疆锛欬/p>

姝ラ	寰幆鏁?/p>	娓╁害	鏃堕棿	鏀堕泦鑽у厜淇″彿
1	1 cycle	95鈩傸/p>	10min	鍚?/p>
2	40 cycles	94鈩傸/p>	15sec	鍚?/p>
2	40 cycles	55鈩傸/p>	30sec	鏄?/p>