瑗垮疂鐢熺墿浠ｇ悊WAKO Phos-tag绯诲垪浜у搧锛屽寘鎷?/span>Phos-tag? Acrylamide Phos-tag鑱氫笝鐑叞鑳裹/span>, SuperSepTM Phos-tag?纾烽吀鍖栬泲鐧界數娉颤/span>棰勫埗鑳迭/span>, Phos-tag? Biotin鐢熺墿绱燑/span>鍏辫江鐨凕/span>Phos-tag锛岀敤浜庣７閰稿寲铔嬬櫧鐨凕/span>WB妫€娴婞/span>, Phos-tag? Agarose鏌卞眰鏋愬垎绂荤７閰稿寲铔嬬櫧銆侟br/>

纾烽吀鍖栬泲鐧界數娉颤/span>璇曞墏鈥斺€擯hos-tagTM

铔嬬櫧璐ㄧ殑纾烽吀鍖栦慨楗版槸鐢熺墿浣撳唴閲嶈鐨勫叡浠风炕璇戝悗淇グ,鍙皟鎺х洰鏍囪泲鐧界殑鍔熻兘锛屽畾浣嶏紝鐗瑰紓鎬х粨鍚堛€侟/span>

铔嬬櫧璐ㄧ殑纾烽吀鍖栫姸鎬侊紙涔熺О纾烽吀铔嬬櫧缁勫锛夊浜庤瘎浼颁笉鍚岀殑鐢熺墿鍜岀梾鐞嗚繃绋嬮潪甯搁噸瑕併€侟/span>2002骞达紝骞垮矝澶у鐨勫皬姹犳暀鎺堝強鍏跺洟闃熷彂琛ㄤ簡涓€涓弻鐜噾灞炵粶鍚堢墿锛圏/span>1,3-bis[bis(pyridin-2-ylmethyl)amino]propan-2-olato dizinc(II) complex)鍦ㄤ腑鎬ф潯浠朵笅浣滃彲閫夋嫨鎬х粨鍚堢７閰稿寲鏍囩鍒嗗瓙锛屽嵆Phos-tagTM銆傛鍚庯紝鍚勭纾烽吀铔嬬櫧璐ㄧ粍瀛︾爺绌舵柟娉曪紝鍦ㄤ娇鐢?/span>Phos-tagTM鐨勮鐢熺墿鍚庡緱鍒颁簡鍙戝睍銆侟/span>

Phos-tagTM绯诲垪S鏄壒寮傛€х粨鍚堢７閰稿寲绂诲瓙鐨勪竴绉嶅姛鑳芥€у垎瀛愶紝鍏锋湁浠ヤ笅鐗圭偣锛欬/span>
1.涓庣７閰哥瀛愶紙2-锛夌殑閫夋嫨鎬х粨鍚堣兘鍔涘己浜庡叾浠栭槾绂诲瓙
2.鍦ㄧ敓鐞嗘潯浠朵笅锛圏/span>PH5-8锛夊舰鎴愮ǔ瀹氱殑鍖栧悎鐗?/span>

1. Phos-tag? Acrylamide

Phos-tag?Acrylamide SDS-PAGE鍙牴鎹縼绉荤巼涓嶅悓锛屽尯鍒嗙７閰稿寲铔嬬櫧涓庨潪纾烽吀鍖栬泲鐧姐€傝鏂规硶鍙渶鍦ㄥ父瑙勭殑SDS-PAGE鑳朵腑娣诲姞Phos-tag?Acrylamide鍜孅/span>MnCl2鍗冲彲杩涜瀹為獙锛堝埗鑳惰繃绋嬩腑娣诲姞锛屽闇€璇︾粏璇存槑璇疯仈绯绘垜浠級銆侟/span>

鐗圭偣

#闈炴斁灏勬€у厓绱狅紝闈炶崸鍏夌墿璐?/span>
#纾烽吀鍖栬泲鐧戒簹鍨嬪彲浠ュ湪鍚屼竴鐢垫吵閬撳垎绂诲嚭澶氭潯甯?/span>
#鎿嶄綔绠€渚匡紝涓庡父瑙凕/span>SDS-PAGE铔嬬櫧鐢垫吵鐩镐技
# Phos-tag?鐨勭粨鍚堢壒寮傛€т笌姘ㄥ熀閰哥绫汇€佸簭鍒楁棤鍏颤/span>
# Phos-tag? SDS-PAGE瀹為獙鍚庯紝鍙繘琛屽父瑙勭殑鍏嶇柅缁勫寲銆佽川璋辩瓑
#鎬ц川绋冲畾锛屾憾浜庤捀棣忔按鍚庡彲浠ョǔ瀹氫繚瀛樿嚦灏慄/span>3涓湀
#纾烽吀鍖栧拰闈炵７閰稿寲铔嬬櫧鍙互鍚屾椂琚垎绂绘娴婞/span>
#涓嶅悓浣嶇偣纾烽吀鍖栦慨楗颁互鍙婄７閰稿寲绋嬪害涓嶅悓鐨勮泲鐧藉湪鍚屼竴娉抽亾涓洜杩佺Щ鐜囦笉鍚岃€岃鍒嗙寮€鏉?/span>

鍘熺悊

搴旂敤

3. Phos-tag? Biotin鐢熺墿绱燑/span>鍏辫江鐨凕/span>Phos-tag锛岀敤浜庣７閰稿寲铔嬬櫧鐨凕/span>WB妫€娴婞/span>

鐢ㄤ簬鍏嶇柅鍗拌抗妫€娴嬬７閰稿寲铔嬬櫧

鐗圭偣锛欬/span>
1.鍙敤浜庢墍鏈夌殑纾烽吀鍖栬泲鐧芥娴婞/span>
2.涓庢櫘閫欬/span>WB妫€娴嬬浉浼笺€侟strong>浣嗘棤闇€浣跨敤纾烽吀鍖栨姉浣掽/strong>

4. Phos-tag? Agarose鏌卞眰鏋愬垎绂荤７閰稿寲铔嬬櫧

鐢ㄤ簬纾烽吀鍖栬泲鐧界殑鏌卞眰鏋愮函鍖朁/span>
Phos-tag? Agarose鍙敤浜庣７閰稿寲铔嬬櫧閫氳繃鏌卞眰鏋愬瘜闆嗭紝鍒嗙锛岀函鍖栥€備笉浣跨敤琛ㄩ潰娲绘€у墏鎴栬繕鍘熷墏锛岀７閰稿寲铔嬬櫧鍙互鍦ㄤ竴涓被浼间綋鍐呯幆澧冧笅鑾峰緱銆侟/span>

鐗圭偣锛欬/span>

1.绾寲鏃堕棿1灏忔椂
2.铔嬬櫧鍙湪鐢熺悊鏉′欢锛圏/span>PH7.5锛変笅鎹曡幏
3.鏃犻渶琛ㄩ潰娲绘€у墏鍜岃繕鍘熷墏