CCK-8试剂盒可用于简便而准确的细胞增殖和细胞毒性分析。
应用【Application】 :
Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.
保质期【shelf life】 :
limited shelf life, expiry date on the label
装运【shipped in】 :
dry ice
存储温度【storage temp.】 :
-20℃
个人防护装备【Personal Protective Equipment】 :
Eyeshields, Gloves
德国水质危险性分类【WGK Germany】 :
3