Catalog# Product Description |
ICP9806 Affinity purified rabbit polyclonal phospho-serine antibodies are developed using a phospho-serine-KLH conjugate and phosvitin mixture as the immunogen. The phospho-serine specific antibodies were affinity purified with serine-phosphorylated peptide immobilized on agarose. This affinity purified antibody showed higher affinity than phospho-serine antibodies from another source. |
|
 Detection of a phosphorylated protein kinase with anti-phosphoserine (ICP9806):Lane1-2: Kinase untreatedLane 3-6: kinase treated with phosphatase PP1G Western blot analysis of phosphorylated proteins with UV-treated mouse cell lysates.A: ControlB-D: Treatment with increased UV doseE: Phosphatase inhibitor treatment High affinity anti-phospho-serineantibody recognizes phospho-proteins (Carbonic anhydrase, phosvitin andα-casein) but not non-phospho proteins (ovalbumin, BSA,β-lactoglobulin, trypsinogen) |
| Species |
Rabbit |
| Formulation |
250 μg/mL in PBS, 50% glycerol, pH 7, 0.01% NaN3 |
| Immunogen |
Phospho-serine KLH and phosvitin mixture |
| Purification |
Affinity purified with phospho-tyrosine on agarose |
| Specificity |
The antibody recognizes proteins and peptides phosphorylated on serine residues. There are no cross reactions with phospho-tyrosine. |
| Tested |
Human breast cancer tissue and mouse cell |
| Applications |
ELISA (1:250); WB (1:250); IP (10 μg/mg crude protein); IHC (1:50) |
| Scientific Description |
Serine is one of the 20 naturally occurring amino acids. Kinases phosphorylate serine to phospho-serine which is responsible for various signalling processes in eukaryotes. |
| Storage & Stability |
Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. |
Product Specific References |
1.Curr. Opin. Cell. Bio.2001.13(2): 131-138.doi:10.1016/S0955-0674(00)00189-7 2.Nat. Commun. 2016.7: 11151. doi:10.1038/ncomms11151 3.Nature Communications. 2016. 7:11151: 1-15. doi:I: 10.1038/ncomms11151
4.Front Immunol.2017; 8: 352. doi: 10.3389/fimmu.2017.00352. 5.Front Immunol.2017; 8: 189. doi:10.3389/fimmu.2017.00189. 6.JAHA. 2018;7(1) doi:10.1161/JAHA.117.007185 7.AJHG. 2018.102(4): 649-657. doi:10.1016/j.ajhg.2018.02.015 8.JAHA. 2018.7(1). doi:10.1161/JAHA.117.007185 9.Cellular Physiology and Biochemisty. 2018.46(1). doi:10.1159/000488418 10.Cell Reports. 2019.28(3): 235-245. doi:10.1016/j.celrep.2019.06.045 |
m.cnreagent.com
