MDL编号(化学反应和变化识别号)【MDL number】 : MFCD00130922
备注【Preparation Note】 : This product is extracted using a method that causes shearing yielding a highly polymerized mixture of double and single stranded DNA . However, double stranded DNA is the predominant form.
This product is prepared from calf thymus tissue of unspecified gender.
品质【Quality】 : "Highly Polymerized"
配制【Reconstitution】 : Solutions of DNA have been stored successfully for several months at 4 C, pH 7.5-8, in 10 mM Tris with 1 mM EDTA and without a bacteriostatic agent. At low concentrations (μg/ml) DNA tends to absorb onto the surfaces of plastic tubes. It is not recommended to store DNA in highly alkaline solutions since DNA tends to degrade at alkaline pH greater than 8.0.
This product can be dissolved at 2 mg/ml in water. To reduce further shearing of the DNA, no sonication or stirring should be used. Gentle inversion overnight at 0-4 ℃ is recommended to completely solubilize the DNA. The presence of 1 mM EDTA is recommended to prevent nucleases from degrading the DNA.
物理形态【Physical form】 : fibrous preparation
应用【Application】 : Calf thymus DNA (CT-DNA) is a natural DNA widely used in studies of DNA binding anticancer agents and DNA binding agents that modulate DNA structure and function. Calf thymus DNA is also used in physicochemical studies of DNA behavior in solution.
物理性质【Physical properties】 : λmax 259 nm (100 mM phosphate buffer, pH 7.0).
DNA from calf thymus is 41.9 mole % G-C and 58.1 mole % A-T.1 An OD of 1.0 at 260 nm corresponds to ~ 50 μg of double-stranded DNA.
质量标准【Quality Level】 : PREMIUM
类型【type】 : Type I
形态【form】 : fibers
颜色【color】 : white
存储温度【storage temp.】 : 2-8℃
安全信息：
个人防护装备【Personal Protective Equipment】 : Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
德国水质危险性分类【WGK Germany】 : 3