HCT-8/FU浜虹粨鑲犵檶姘熷翱鍢у暥鑰愯嵂鏍?/strong>鐜拌揣渚涘簲锛屼环鏍间紭鎯犮€佸疄楠屾晥鏋滃ソ锛屾垜鍙镐负鎮ㄦ彁渚涘厤璐逛唬娴嬫湇鍔★紝鍚屾椂鎻愪緵鏈€鏂颁环鏍笺€佽鏄庝功銆佽鏍笺€佺敤閫斻€佸疄楠屽師鐞嗙瓑鐩稿叧鎿嶄綔璇存槑銆侟br/>

浜у搧鍚嶇О	HCT-8/FU浜虹粨鑲犵檶姘熷翱鍢у暥鑰愯嵂鏍?/strong>
浜у搧绠€绉?/td>	HCT-8/FU
璐у彿	GOY-N0002

缁嗚優鐨勭绫伙細

绗楃锛欬/p>

鏄喕瀛樹骇鍝侊紝鐢辨恫姘洿鎺ユ嬁鍑猴紝骞插啺杩愯緭缁欏鎴枫€備竴鑸笉鎺ㄨ崘杩欑锛屼篃杈冨皯浣跨敤杩欑鏂瑰紡锛屽洜涓哄鑻忔墜娉曞浜庣粏鑳炵姸鎬佸奖鍝嶈緝澶э紝涓€鏃︾粏鑳炵姸鎬佷笉濂斤紝寰堥毦璇存槸缁嗚優鑷韩涓嶈锛岃繕鏄鑻忔病鎿嶄綔濂斤紱鍙﹀娓╁害瀵圭粏鑳炪€佸熀鍥犲姛鑳界姸鎬佸奖鍝嶅緢澶э紝涔熶笉鏄粏鑳炲偍瀛樼殑zui濂芥柟寮忥紝蹇€掓椂闂翠篃寰堥毦鎺у埗锛屽绉嶅洜绱犲奖鍝嶄骇鍝佺殑璐ㄩ噺锛涘共鍐拌繍杈撻渶瑕侀澶栨坊鍔?00鍏冪殑杩愯垂(椤轰赴)銆侟/p>

绗簩绉嶏細

鏄垜浠鑻忓ソ缁嗚優锛孮C閫氳繃鍚庯紝T-25鐏屾弧鍩瑰吇鍩哄父娓╄繍杈撶粰瀹㈡埛锛屾帓闄ゅ鑻忛€犳垚褰卞搷锛屽父娓╄繍杈撲篃瀵圭粏鑳炲奖鍝嶄篃涓嶅ぇ锛屽彧闇€鍔?5鍏冭繍璐?椤轰赴)銆侟/p>

璐ㄩ噺淇濊瘉锛氭垜浠殑缁嗚優鏍潵婧愪簬ATCC銆丒CACC銆丏SMZ銆丣CRB绛夛紝璐拱鍒拌揣鍚庯紝鐢辨垜浠疄楠屽鎵╁銆佸喕瀛橈紝鍐诲瓨鐨勪骇鍝佸叏閮ㄧ粡杩嘠C妫€娴嬶紝100%杩涘彛鏉ユ簮锛?00%淇濊瘉5浠ｄ互鍐咃紝娲诲姏>95%锛屾棤缁嗚弻銆佺湡鑿屻€佹敮鍘熶綋姹℃煋銆傜殑鍐诲瓨鏄粏鑳炲煿鍏荤殑鍩烘湰鎿嶄綔锛屼笉鍚岀殑瀹為獙瀹ら噰鐢ㄧ殑鏂规硶鐣ユ湁宸紓锛屼絾鏄兘浼氶伒寰參鍐婚€熸憾鐨勫畻鏃ㄣ€侟/p>

銆愬煿鍏绘寚鍗椼€慄/span>

瀵逛簬璐村缁嗚優锛屼紶浠ｅ彲鍙傝€冧互涓嬫柟娉曪細

1. 寮冨幓鍩瑰吇涓婃竻锛岀敤涓嶅惈閽欍€侀晛绂诲瓙鐨凱BS娑︽礂缁嗚優1-2娆°€侟/p>

2. 鍔?ml娑堝寲娑?0.25%Trypsin-0.53mM EDTA)浜庡煿鍏荤摱涓紝缃簬37鈩冨煿鍏荤涓秷鍖?-2鍒嗛挓锛岀劧鍚庡湪鏄惧井闀滀笅瑙傚療鈪″瀷鑲烘场涓婄毊缁嗚優|缁嗚優娑堝寲鎯呭喌锛岃嫢缁嗚優澶ч儴鍒嗗彉鍦嗗苟鑴辫惤锛岃繀閫熸嬁鍥炴搷浣滃彴锛岃交鏁插嚑涓嬪煿鍏荤摱鍚庡姞灏戦噺鍩瑰吇鍩虹粓姝㈡秷鍖栥€侟/p>

3. 鎸?-8ml/鐡惰ˉ鍔犲煿鍏诲熀锛岃交杞绘墦鍖€鍚庡惛鍑猴紝鍦?000RPM鏉′欢涓嬬蹇?鍒嗛挓锛屽純鍘讳笂娓呮恫锛岃ˉ鍔?-2mL鍩瑰吇娑插悗鍚瑰寑銆侟/p>

4. 灏嗙粏鑳炴偓娑叉寜1锛?鍒?锛?鐨勬瘮渚嬪垎鍒版柊鐨勫惈8ml鍩瑰吇鍩虹殑鏂扮毧涓垨鑰呯摱涓€侟/p>

銆愮粏鑳炲喕瀛樸€慄/span>

浜у搧鍚嶇О寰呯粏鑳炵敓闀跨姸鎬佽壇濂芥椂锛屽彲杩涜293/HEK-293缁嗚優|缁嗚優鍐诲瓨銆傝创澹佺粏鑳炲喕瀛樻椂锛屽純鍘诲煿鍏诲熀鍚庡姞鍏ュ皯閲忚儼閰讹紝缁嗚優鍙樺渾鑴辫惤鍚庯紝鍔犲叆绾?ml鍚娓呯殑鍩瑰吇鍩哄悗鍔犲叆鍐诲瓨绠′腑锛屽啀娣诲姞10%DMSO鍚庤繘琛屽喕瀛樸€傛偓娴粏鑳炲喕瀛樻椂锛屽簲灏嗙粏鑳炴敹闆嗭紝1000RPM鏉′欢涓嬬蹇?鍒嗛挓锛屽皯閲忎繚瀛樹笂娓呮恫(闃叉缁嗚優鍚歌蛋)锛屽姞鍏ラ儴鍒嗘柊椴滃煿鍏诲熀锛屽姞鍏ュ埌鍐诲瓨绠′腑锛屽湪鍐诲瓨绠′腑鍔犲叆10%DMSO鍚庤繘琛屽喕瀛樸€侟/p>

銆愬鑻忕殑鍘熷垯銆慄/strong>

蹇€熻瀺鍖栵細蹇呴』灏嗗喕瀛樺湪-196鈩冩恫姘腑鐨勭粏鑳炲揩閫熻瀺鍖栬嚦37鈩冿紝浣跨粏鑳炲鍐诲瓨鏃剁殑鍐版櫠杩呴€熻瀺鍖栵紝閬垮厤鍐版櫠缂撴參铻嶅寲鏃惰繘鍏ョ粏鑳炲舰鎴愬啀缁撴櫠锛屽缁嗚優閫犳垚鎹熷銆傚鏃︾粏鑳炲簱鍏ㄥ浗鍚勫湴鍧囧彲鍙戣揣锛屽叏鍥界粺涓€浠锋牸锛屾湰鍏徃鍑哄敭鐨勬墍鏈夌粏鑳炰粎渚涚鐮斾娇鐢ㄣ€侟br/>

TSA Agar鑳拌泲鐧借儴澶ц眴鐞艰剛锛圱SA锛堻span style="white-space: pre;">250g

Enterobacter Sakazakii Chromogenic Medium闃磶鏉嗚弻鏄捐壊鍩瑰吇鍩裹span style="white-space:pre">1000ml

Brain Heart Infusion Broth鑴戝績娴告恫鑲夋堡250g

Modified lauryl sulfate tryptose vancomycin medium鏀硅壇鏈堟鍩虹～閰哥洂鑳拌泲鐧借儴鑲夋堡-涓囧彜闇夌礌锛坢LST-Vm锛堻span style="white-space:pre">250g

Vancomycin Solution涓囧彜闇夌礌婧舵恫1mg*5鏀?/p>

VRBG AgarVRBG鐞艰剛250g

Simmons Citrate Medium瑗胯挋姘忔灨姗奸吀鐩愬煿鍏诲熀250g

biochemical identification of sets of tubes闃磶鑲犳潌鑿屾垚濂楃敓鍖栭壌瀹氱11绉?2濂?鐩?5鐩扅/p>

姘у寲閰惰瘯绾伏span style="white-space:pre">10鐗嘃/p>

Enterobacter Sakazakii Isolation Agar闃磶鑲犳潌鑿屽垎绂荤惣鑴傦紙ESIATM锛堻span style="white-space:pre">250g

Enterobacter Sakazakii Isolation Agar闃磶鏉嗚弻鍒嗙鐞艰剛锛圗SIATM锛堻span style="white-space:pre">250g

鑳拌泲鐧借儴澶ц眴鐞艰剛5kg/琚婞/p>

鑲犵悆鑿屻€佹憾琛€鎬ч摼鐞冭弻鍜岀勃閾剧悆鑿屾楠屽煿鍏诲熀

Dextrose Meat Infusion Broth钁¤悇绯栬倝娴告恫鑲夋堡250g

Pick鈥檚 Broth BaseA鍖瑰厠姘忚倝姹ゅ熀纭€A100g

Meat Infusion Broth鑲夋蹈娑茶倝姹ゅ煿鍏诲熀250g

Azide Dextrose Broth鍙犳爱閽犺憽钀勭硸鑲夋堡250g

Ethyl Violet Aziode Broth涔欏熀绱彔姘挔鑲夋堡250g

KF Streptococcus AgarKF閾剧悆鑿岀惣鑴侟span style="white-space:pre">100

Nalidixic Acid钀樺暥閰吀1.5mg/鏀?5

Enterococcus Agar鑲犵悆鑿岀惣鑴侟span style="white-space:pre">250g

Filter Membrane Enterococcus Agar婊よ啘鑲犵悆鑿岀惣鑴侟span style="white-space:pre">250g

CATC AgarCATC鐞艰剛250g

Bile Esculin Agar鑳嗙洂涓冨彾鑻风惣鑴侟span style="white-space:pre">250g

CD100鍒嗗瓙RNA闀挎晥淇濆瓨娑秉span style="white-space: pre;">RNASAVERCEACAM6 / CD66c 鎶楅珨, 鍏斿柈鎶桙/p>

CD133鍒嗗瓙RNase鎶戝埗鍓傦紙浜烘簮锛堻span style="white-space:pre">Human RNase InhibitorCD20 / MS4A1 鎶楅珨 (APC), 鍏斿柈鎶桙/p>

CD146鍒嗗瓙RNase鎶戝埗鍓傦紙浜烘簮锛堻span style="white-space:pre">Human RNase InhibitorCD2 鎶楅珨 (APC), 鍏斿柈鎶桙/p>

CD14鍒嗗瓙RNase鎶戝埗鍓傦紙灏忛紶婧愶級Murine RNase InhibitorHER2 / ErbB2 / CD340 鎶楅珨, 鍏斿柈鎶桙/p>

CD163鍒嗗瓙RNase鎶戝埗鍓傦紙鐚簮锛堻span style="white-space:pre">Coagulation Factor XIII B chain / F13B 鎶楅珨, 鍏斿柈鎶桙/p>

CD20鍒嗗瓙闈為叾DNA娓呴櫎鍓侟span style="white-space:pre">DNA ErasolHER2 / ErbB2 / CD340 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

CD28鍒嗗瓙闈為叾DNA娓呴櫎鍓?2DNA Erasol-2CD147 / EMMPRIN / Basigin 鎶楅珨, 鍏斿鎶桙/p>

CD30鍒嗗瓙鏌卞紡DNA娓呴櫎鍓侟span style="white-space:pre">Column DNA ErasolCD155 / PVR / NECL5 鎶楅珨, 鍏斿鎶桙/p>

CD38鍒嗗瓙澶ф彁鏌卞紡DNA娓呴櫎鍓侟span style="white-space:pre">Maxi Column DNA ErasolCD208 / LAMP3 / DC-LAMP 鎶楅珨, 鍏斿鎶桙/p>

CD3鍒嗗瓙鏃燫Nase鐨凞Nase婧舵恫,1U/渭LRNase-Free DNase SolutionHER3 / ErbB3 鎶楅珨, 鍏斿鎶桙/p>

CD40鍒嗗瓙M鑶滅粨鍚圖NA娓呴櫎璇曞墏鐩扅span style="white-space:pre">Membrane-Bound DNA ErasolAGO2 / Argonaute 2 鎶楅珨, 鍏斿鎶桙/p>

CD40閰嶄綋闈為叾澶氱硸娓呴櫎鍓傦紙RNA涓撶敤锛堻span style="white-space:pre">PS Erasol锛圧NA锛堻span style="white-space:pre">Gastric intrinsic factor / GIF 鎶楅珨, 鍏斿鎶桙/p>

CD44鍙樹綋6TRIzol浼翠荆TRIzol-MateREG3A / HIP 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

HCT-8/FU浜虹粨鑲犵檶姘熷翱鍢у暥鑰愯嵂鏍?/strong>CD44鍒嗗瓙閲嶈捀閰欬span style="white-space:pre">Redistilled PhenolH3F3A 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

CD4鍒嗗瓙姘撮ケ鍜岄厷Water-Saturated PhenolASF1A 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

CD8鍒嗗瓙Tris楗卞拰閰欬span style="white-space:pre">Tris-Saturated PhenolARIH2 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

CD铔嬬櫧(鎹熷潖鎬у叧鑺傜値锛堻span style="white-space:pre">閰搁厷Acidic PhenolGSTP1 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

CIP2A姘豢-寮傛垔閱囨贩鍚堟恫Chloroform-Isoamyl AlcoholELAVL1 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

c-Jun姘ㄥ熀鏈婵€閰迭span style="white-space:pre">閰?姘豢-寮傛垔閱囨贩鍚堟恫Phenol-Chloroform-IsoamylolFOXA1 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

Clara缁嗚優鍒嗘硨铔嬬櫧瓒呯函姘滁span style="white-space:pre">Ultrapure WaterHAT1 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

C-Raf鍘熺檶鍩哄洜涓濊嫃姘ㄩ吀铔嬬櫧婵€閰迭span style="white-space:pre">DEPC姘滁span style="white-space:pre">DEPC-Treated WaterGATAD2A / Hp66alpha 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

CREB璋冭妭杞綍杈呮縺娲诲洜瀛?RNase-free 姘滁span style="white-space:pre">RNase-free WaterSTMN1 / Stathmin 1 鎶楅珨, 鍏斿鎶? 鎶楀師瑕拰绱斿寲

缁嗚優鍩瑰吇鐨勪紭鐐癸細

1.鐮旂┒鐨勫璞℃槸娲荤粏鑳濣/p>

鍦ㄥ疄楠岃繃绋嬩腑锛屾牴鎹姹傚彲濮嬬粓淇濇寔缁嗚優娲诲姏锛屽苟鍙暱鏃堕棿鐩戞帶銆佹娴嬬敋鑷冲畾閲忚瘎浼颁竴閮ㄥ垎娲荤粏鑳炵殑鎯呭喌锛屽寘鎷椿缁嗚優鐨勫舰鎬併€佺粨鏋勩€佺敓鍛芥椿鍔ㄧ瓑銆侟/p>

2.鐮旂┒鏉′欢鍙互浜轰负鎺у埗

pH銆佹俯搴︺€佹哀姘斻€佷簩姘у寲纰炽€佸紶鍔涚瓑鐗╃悊鍖栧鐨勬潯浠讹紝鍙互鏍规嵁瀹為檯闇€瑕佽繘琛屼汉涓虹殑鎺у埗锛屽悓鏃讹紝鍙互鏂藉姞鍖栧銆佺墿鐞嗐€佺敓鐗╃瓑鍥犵礌浣滀负鏉′欢鑰岃繘琛屽疄楠岃瀵燂紝杩欎簺鍥犵礌鍚屾牱鍙互澶勪簬涓ユ牸鎺у埗涔嬩笅銆侟/p>

3.鐮旂┒鐨勬牱鏈彲浠ヨ揪鍒版瘮杈冨潎涓€鎬?/p>

閫氳繃缁嗚優鍩瑰吇涓€瀹氫唬鏁板悗锛屾墍寰楀埌鐨勭粏鑳炵郴鍒欏彲浠ヨ揪鍒板潎涓€鎬ц€屽睘浜庡悓涓€绫诲瀷鐨勭粏鑳烇紝闇€瑕佹椂锛屽彲閲囩敤鍏嬮殕鍖栫瓑鏂规硶浣跨粏鑳炶揪鍒扮函鍖栥€侟/p>

4.鐮旂┒鍐呭渚夸簬瑙傚療銆佹娴嬪拰璁板綍

閲囩敤鍚勭鐮旂┒鎶€鏈細濡傚€掔疆鐢熺墿鏄惧井闀溿€佽崸鍏夋樉寰暅銆佺數瀛愭樉寰暅銆佹祦寮忕粏鑳炴湳銆佹縺鍏夊叡鐒︽樉寰暅銆佸厤鐤粍缁囧寲瀛︺€佸師浣嶆潅浜ゃ€佸悓浣嶇礌鏍囪绛夊悇绉嶄华鍣ㄨ澶囥€侟br/>