• Automated sequencing of all DNA templates, including those containing difficult-to-sequence regions that cannot be resolved with other sequencing kits (Figs. 1-4).

• 瀵规墍鏈塂NA妯℃澘杩涜鑷姩娴嬪簭锛屽寘鎷偅浜涘惈鏈夐毦浠ョ敤鍏朵粬娴嬪簭璇曞墏鐩掕В鏋愮殑闅句互娴嬪簭鍖哄煙鐨凞NA妯℃澘锛堝浘1-4锛夈€侟/span>

SequiTherm EXCEL II? Sequencing Kits for automated sequencers use the SequiTherm EXCEL II Polymerase, which improves sequencing performance with increased signal intensities and the ability to sequence through GC-rich regions. The SequiTherm EXCEL II DNA Sequencing Kits incorporate the use of betaine,* which enables sequencing of a broad range of templates, including those containing regions that are very difficult to sequence. Protocols are provided for both cycle and high-temperature isothermal (noncycle) sequencing.

鐢ㄤ簬鑷姩娴嬪簭浠殑SequiTherm EXCEL II?娴嬪簭璇曞墏鐩掍娇鐢⊿equiTherm EXCEL II鑱氬悎閰讹紝璇ラ叾鍙彁楂樻祴搴忔€ц兘锛屽悓鏃跺鍔犱俊鍙峰己搴︼紝骞惰兘澶熼€氳繃瀵屽惈GC鐨勫尯鍩熻繘琛屾祴搴忋€侟/span>SequiTherm EXCEL II DNA娴嬪簭璇曞墏鐩掔粨鍚堜簡鐢滆彍纰辩殑浣跨敤锛?鍙互瀵瑰绉嶆ā鏉胯繘琛屾祴搴忥紝鍖呮嫭閭ｄ簺寰堥毦娴嬪簭鐨勫尯鍩熴€侟/span>鎻愪緵浜嗙敤浜庡惊鐜拰楂樻俯绛夋俯锛堥潪寰幆锛夋祴搴忕殑鏂规銆侟/span>

The kits have been optimized for use with the following DNA sequencers: LI-COR? 4000 and 4200 series Sequencers, NEN? Global IR²DNA Sequencers, ALF? DNA Sequencers, and ABI PRISM? DNA Sequencers using dye primer technology. Using primers labeled with standard sequencing dyes, the SequiTherm EXCEL II kits may also be used with ABI PRISM 373 and 377 series sequencers. The kits are not compatible with ABI capillary sequencers (ABI 310, 3100, or 3700 series) or with BigDye? technology.

璇ヨ瘯鍓傜洅缁忚繃浼樺寲锛屽彲涓庝互涓婦NA娴嬪簭浠厤鍚堜娇鐢細LI-COR?4000鍜?200绯诲垪娴嬪簭浠紝NEN?Global IR²DNA娴嬪簭浠紝ALF?DNA娴嬪簭浠拰浣跨敤鏌撴枡寮曠墿鎶€鏈殑ABI PRISM?DNA娴嬪簭浠€侟/span>浣跨敤鏍囨湁鏍囧噯娴嬪簭鏌撴枡鐨勫紩鐗╋紝SequiTherm EXCEL II璇曞墏鐩掕繕鍙笌ABI PRISM 373鍜?77绯诲垪娴嬪簭浠竴璧蜂娇鐢ㄣ€侟/span>璇ュ浠朵笌ABI姣涚粏绠℃祴搴忎华锛圓BI 310銆?100鎴?700绯诲垪锛夋垨BigDye?鎶€鏈笉鍏煎銆侟/span>

Two different kits are available for use with LI-COR? and NEN? sequencing equipment. The first kit (Cat. No. SE9101LC) contains Termination Mixes that give optimal read lengths up to about 1,000 bases and is designed for use on 25- to 41-cm gels. The second kit (Cat. No. SE9202LC) provides enhanced signal intensities in the resolution range of 1,000-1,400 bases and is ideal for users interested in obtaining the longest possible reads in conjunction with 66-cm gels, version 4.x of the BaseImagIR? software, and all versions of e-Seq? software.

鏈変袱绉嶄笉鍚岀殑濂椾欢鍙笌LI-COR?鍜孨EN?娴嬪簭璁惧涓€璧蜂娇鐢ㄣ€侟/span>绗椾釜璇曞墏鐩掞紙鐩綍鍙稴E9101LC锛夊寘鍚粓姝㈡贩鍚堢墿锛屽彲鎻愪緵闀胯揪1000涓⒈鍩虹殑鏈€浣宠鍙栭暱搴︼紝骞惰璁＄敤浜?5鑷?1鍘樼背鐨勫嚌鑳躲€侟/span>绗簩涓瘯鍓傜洅锛堢洰褰曞彿SE9202LC锛夊湪1,000-1,400纰卞熀鐨勫垎杈ㄧ巼鑼冨洿鍐呮彁渚涘寮虹殑淇″彿寮哄害锛屽浜庡笇鏈涗笌66-cm鍑濊兌锛堢増鏈?.x鐨凚aseImagIR锛変竴璧疯幏寰楀敖鍙兘闀跨殑璇绘暟鐨勭敤鎴锋潵璇存槸鐞嗘兂鐨勯€夋嫨?杞欢浠ュ強鎵€鏈夌増鏈殑e-Seq?杞欢銆侟/span>

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