HyperMu? Insertion Kit epicentre HMI032K 半岛bd体育手机客户端 描述

应用领域

• Completely sequence even the largest cDNA or genomic clones without subcloning or primer walking.

• 甚至可以对最大的cDNA或基因组克隆进行完全测序，而无需亚克隆或引物步移。

HyperMu? and Insertion Kits are designed to simplify and speed up sequencing any cloned DNA over 2 kb. These kits use HyperMu? MuA Transposase, a hyperactive mutant MuA transposase with an in vitrotransposition efficiency at least 50 times higher than similar enzymes from other sources.

HyperMu? 和 插入试剂盒旨在简化并加快测序任何2 kb以上克隆的DNA的速度。 这些试剂盒使用HyperMu?MuA转座酶，一种高活性的突变MuA转座酶，其体外转座效率比其他来源的类似酶高至少50倍。

Like the EZ-Tn5? Transposon Insertion Kits, the HyperMu Insertion Kits simplify and speed up complete sequencing of any cloned DNA that is too large to sequence with a single set of sequencing reactions.

像EZ-Tn5?转座子插入试剂盒一样，HyperMu插入试剂盒可简化并加快对所有克隆的DNA的完整测序，这些DNA太大而无法通过一套测序反应进行测序。

The process for sequencing even the largest BAC clone without the time and expense of subcloning or primer walking is shown in Fig. 1. First, the HyperMu Transposon is randomly inserted into the target DNA using a simple, 2-hourin vitroreaction catalyzed by HyperMu Transposase. Then, following transformation ofE. coliand plating on medium containing either kanamycin or chloramphenicol, up to a million independent insertion clones are obtained. The complete sequence of the target DNA is easily obtained by sequencing selected insertion clones bidirectionally using the two primers in the kit that are homologous to the ends of the inserted HyperMu Transposon.

图1显示了即使最大的BAC克隆的测序过程，也无需花费时间和亚克隆或引物步移的时间和费用。首先，HyperMu转座子可通过简单的2小时体外体外反应（通过HPC催化）随机插入目标DNA中。 HyperMu转座酶。然后，在转化大肠杆菌并将其铺板于含有卡那霉素或氯霉素的培养基上之后，获得了多达一百万个独立的插入克隆。通过使用试剂盒中与插入的HyperMu转座子末端同源的两个引物双向选择选定的插入克隆，可以轻松获得目标DNA的完整序列。

好处说明

Benefits

• A single reaction generates enough insertion clones to sequence even the largest BAC clone.

• 一个反应即可生成足够的插入克隆，甚至可以对最大的BAC克隆进行测序。

• Use the sequencing primers provided in the kit to completely sequence your clone; no additional primer synthesis required.

• 使用试剂盒中提供的测序引物对您的克隆进行完全测序；无需额外的引物合成。

• Insertion specificity is highly random, ensuring complete sequence coverage.

• 插入特异性是高度随机的，可确保完整的序列覆盖。