H1浜鸿儦鑳庡共缁嗚優绯狐/span>

浜у搧绠€浠嬶細瑙勬牸锛欬/span>1脳10⁶cells/绠狘/span>

娲荤粏鑳炶繍杈擄細鏀跺埌缁嗚優鍚庯紝璇风珛鍗虫斁鍏ュ煿鍏荤涓煿鍏伙紱

鍐诲瓨缁嗚優杩愯緭锛氬彲鏆傚瓨浜嶞/span>-80鈩傸span style="font-family:寰蒋闆呴粦">鍐扮鎴栨恫姘暱鏈熷偍瀛樸€侟/span>

H1浜鸿儦鑳庡共缁嗚優绯绘槸鍥介檯涓婃渶閫氱敤鑳氳儙骞茬粏鑳炵郴涔嬩竴锛岃缁嗚優鏄粠浜虹被鏃╂湡鑳氳儙鍐呯粏鑳炲洟鍒嗙鍑烘潵锛屽叿鏈変綋澶栧煿鍏绘棤闄愬娈栥€佽嚜鎴戞洿鏂板拰澶氬悜鍒嗗寲鐨勭壒鎬с€傚彲涓虹粏鑳為仐浼犳搷浣滃拰鍒嗗寲鐮旂┒鎻愪緵涓板瘜鐨勫疄楠屾潗鏂欍€傝浜у搧閲囩敤鍩冩辰鎬滭/span>鐢熺墿鐨勪汉澶氳兘骞茬粏鑳炴潯浠跺煿鍏诲熀锛圏/span>AC-1001000/AC-1001001锛堻/span>杩涜缁嗚優鍩瑰吇涓庝紶浠ｃ€傛湰浜у搧缁忔娴婞/span>H1浜鸿儦鑳庡共缁嗚優绯绘棤缁嗚弻銆佺湡鑿屻€侀湁鑿屻€佹敮鍘熶綋姹℃煋锛屾娴嬩负闃存€?/span>銆侟/span>

浜у搧缁勬垚锛欬/span>

浜у搧璐у彿	鍚嶇О	瑙勬牸	鏁伴噺
AC-2001002H1	H1浜鸿儦鑳庡共缁嗚優	1脳106	1绠狘/span>

瀹為獙鏉愭枡

iPS浜裹/span>璇卞澶氳兘骞茬粏鑳烇紙AppliedCell^TM: Cat. no.AC-2001001锛堻/span>

H9浜鸿儦鑳庡共缁嗚優锛圏/span>AppliedCell^TM: Cat. no.AC-2001002H9锛堻/span>

浜哄鑳藉共缁嗚優鍩瑰吇鍩猴紙AppliedCell^TM: Cat. no.AC-1001000锛堻/span>

鍗崇敤鍨嬪熀璐ㄨ兌锛圏/span>AppliedCell^TM: Cat. no.AC-1001007锛堻/span>

浜裹/span>澶欬/span>鑳藉共缁嗚優娑堝寲娑诧紙AppliedCell^TM: Cat. no.AC-1001008锛堻/span>

Serum-Free骞茬粏鑳炲喕瀛樻恫锛圏/span>AppliedCell^TM: Cat. no.AC-1001011/1001012锛堻/span>

纾烽吀缂撳啿鐩愭憾娑秉/span>(Phosphate Buffer Saline锛孅/span>PBS)

鎿嶄綔鏂规硶锛堜互涓嬫楠ょ殕搴斿湪鏃犺弻鏉′欢涓嬫搷浣滐級

H1浜鸿儦鑳庡共缁嗚優澶嶈嫃

1.瀹為獙鎿嶄綔姝ラ锛欬/span>

1.1鍩鸿川鑳迭/span>鍖呰鍩瑰吇鏉军/span>锛氬彇鍑裹/span>6瀛旀澘12瀛旀澘锛屾瘡瀛斿唴鍔犲叆1 mL0.5 mL鍗崇敤鍨嬪熀璐ㄨ兌锛圏/span>AC-1001007锛堻/span>锛岃交杞绘檭鍔?/span>6瀛旀澘12瀛旀澘浣垮熀璐ㄨ兌瀹屽叏瑕嗙洊鐨垮簳锛岀疆浜嶞/span>37鈩傸/span>鍩瑰吇绠变腑瀛佃偛1h~2h锛屽疄楠屽墠鎷垮嚭骞剁疆浜庤秴鍑€宸ヤ綔鍙?/span>/鐢熺墿瀹夊叏鏌滀腑瀹ゆ俯涓嬪钩琛狘/span>20min銆傚鏋滄殏鏃朵笉鐢紝鍙敤Parafilm灏佸彛鍚嶞/span>2-8鈩傸/span>鍌ㄥ瓨锛屽苟浜嶞/span>1鍛ㄥ唴浣跨敤銆侟/span>

娉ㄦ剰锛欬/span>鈶燑span style="font-family:妤蜂綋">涓€鏀喕瀛樼殑骞茬粏鑳炵殑鏁伴噺鍦?/span>1脳10⁶cellsmL宸﹀彸锛孅/span>瀵瑰簲6瀛旀澘1瀛旓紱鈶狘/span>鍗崇敤鍨嬪熀璐ㄨ兌瀵规俯搴︽晱鎰烖/span>锛屽嵆鐢ㄥ嵆鎷军/span>锛岀敤瀹屽悗绔嬪嵆鏀惧洖4鈩傸/span>鍐扮淇濆瓨锛孅/span>涓斿嬁鐢ㄦ墜鐩存帴瑙︾鍩鸿川鑳舵恫闈㈡墍鍙婄殑鐡惰韩锛屽奖鍝嶅熀璐ㄨ兌璐ㄩ噺銆侟/span>

1.2鍏圏/span>灏咟/span>2~3mL鐨凕/span>骞茬粏鑳炲煿鍏诲熀鍔犲叆15mL绂诲績绠狘/span>涓?/span>澶囩敤銆侟/span>

1.3瑙ｅ喕锛氬皢浠庢恫姘腑鍙栧嚭鐨勫喕瀛樼蹇€熸蹈鍏?/span>37鈩傸/span>娓╂按涓紝蹇€熸憞鍔紝浣垮叾鍦?/span>1~2min鍐呭揩閫熻В鍐伙紱

娉ㄦ剰锛氱粏鑳炰粠娑叉爱涓嬁鍑虹殑閫熷害瑕佸揩锛屽敖閲忓噺灏戝叾鏆撮湶鍦ㄥ娓╀腑鐨勬椂闂淬€侟/span>

1.4绂诲績锛氬喕瀛樼涓殑鍐诲瓨娑茶В鍐诲悗锛屽皢鍏堕€愭淮鍔燑/span>鍏?/span>鍚湁骞茬粏鑳炲煿鍏诲熀鐨凕/span>15mL绂诲績绠′腑锛屽皢15mL绂诲績绠＄疆浜庝綆閫熺蹇冩満涓厤姣斿钩琛″悗锛孅/span>1000rpm绂诲績3min锛汓/span>

1.5閲嶆偓锛欬/span>绂诲績鍚庡純涓婃竻鍔燑/span>1mL浜哄鑳藉共缁嗚優鍩瑰吇鍩裹/span>瀵瑰共缁嗚優娌夋穩杩涜鍚瑰惛娣峰寑锛屽惞鍚伏/span>3~5娆″乏鍙炽€侟/span>

1.6鎺ョ锛氬惞鍚稿潎鍖€鍚庯紝灏嗗凡缁忓钩琛″ソ鐨凕/span>鍗崇敤鍨嬪熀璐ㄨ兌寮冩帀锛孅/span>灏嗗惞鎵撳潎鍖€鐨勫共缁嗚優鎮恫鍔犲叆鍒?/span>宸茬粡鍖呰濂界殑6瀛旀澘涓紝骞迭/span>琛ュ叏姣忓瓟2mL鍩瑰吇浣撶郴銆侟/span>

1.7鍩瑰吇锛氭帴绉嶅悗鐨凕/span>6瀛旀澘鍙互缃簬鍊掔疆鐩稿樊鏄惧井闀滀笅瑙傚療鎺ョ鐨勫共缁嗚優瀵嗗害浠ュ強缁嗚優鍥㈠潡澶у皬锛屽憟4涓粏鑳炰互涓婄殑鍥㈠潡杈冨悎鏍硷紝姘村钩鍗佸瓧杞昏交鏅冨姩6瀛旀澘/12瀛旀澘浣跨粏鑳炲潎鍖€鍒嗗竷銆傚苟缃簬37鈩傸/span>锛孅/span>5% CO₂鐨勬亽娓╁煿鍏荤鍩瑰吇锛岀2澶╄瀵熺粏鑳炶创澹佹儏鍐碉紱

1.8鎹㈡恫锛氫粠澶嶈嫃鐨勬椂闂村紑濮嬫瘡24h鎹㈡恫涓€娆°€侟/span>