iPS浜鸿瀵煎鑳藉共缁嗚優 - 半岛电竞官方网址

iPS浜鸿瀵煎鑳藉共缁嗚優

璐у彿:	AC-2001001
鍝佺墝:	鍩冩辰鎬濈敓鐗?/td>
鍙傝€冧环鏍机	3650.52
浜у湴:	涓婃捣

绱㈠彇璧勬枡鍙婃姤浠饵/span>

浜у搧浠嬬粛

iPS浜裹/span>璇卞澶氳兘缁嗚優

瑙勬牸锛欬/span>1脳10⁶cells/绠狘/span>

娲荤粏鑳炶繍杈擄細鏀跺埌缁嗚優鍚庯紝璇风珛鍗虫斁鍏ュ煿鍏荤涓煿鍏伙紱

鍐诲瓨缁嗚優杩愯緭锛氬彲鏆傚瓨浜嶞/span>-80鈩傸span style="font-family:寰蒋闆呴粦">鍐扮鎴栨恫姘暱鏈熷偍瀛樸€侟/span>

浜у搧绠€浠嬶細

iPS浜裹/span>璇卞澶氳兘缁嗚優浜у搧鏄埄鐢ㄧ梾姣掕浇浣撳皢鍥涘洜瀛愮郴缁烖/span>(Oct4銆?/span>Sox2銆Klf4銆?/span>c-Myc)杞叆鐨偆鎴愮氦缁寸粏鑳烇紙鎴愬勾濂虫€э級灏嗗叾璇卞閲嶇紪绋嬩负iPS缁嗚優锛屼笖瀵更/span>iPS缁嗚優杩涜鍒嗗寲鍙婂共缁嗚優marker閴村畾銆侟/span>璇ヤ骇鍝侀噰鐢?/span>鍩冩辰鎬滭/span>鐢熺墿鐨凕/span>浜哄鑳藉共缁嗚優鍩瑰吇鍩裹/span>锛圏/span>AC-1001000/AC-1001001锛堻/span>杩涜缁嗚優鍩瑰吇涓庝紶浠ｃ€傛湰浜у搧缁忔娴婞/span>浜裹/span>iPS缁嗚優鏃犵粏鑿屻€佺湡鑿屻€侀湁鑿屻€佹敮鍘熶綋姹℃煋銆侟/span>

浜у搧缁勬垚锛欬/span>

浜у搧璐у彿	鍚嶇О	瑙勬牸	鏁伴噺
AC-2001001	iPS浜鸿瀵煎鑳键/span>缁嗚優	1脳10⁶	1绠狘/span>

瀹為獙鏉愭枡

H1浜裹/span>鑳氳儙骞秉/span>缁嗚優绯狐/span>锛圏/span>Applied Cell^TM: Cat. no.AC-2001002锛堻/span>

H9浜裹/span>鑳氳儙骞秉/span>缁嗚優绯狐/span>锛圏/span>Applied Cell^TM: Cat. no.AC-2001003锛堻/span>

浜哄鑳藉共缁嗚優鍩瑰吇鍩猴紙Applied Cell^TM: Cat. no.AC-1001000锛堻/span>

Advance浜哄鑳藉共缁嗚優鍩瑰吇鍩猴紙Applied Cell^TM: Cat. no.AC-1001001锛堻/span>

鍗崇敤鍨嬪熀璐ㄨ兌锛圏/span>Applied Cell^TM: Cat. no.AC-1001007锛堻/span>

浜哄鑳藉共缁嗚優娑堝寲娑诧紙Applied Cell^TM: Cat. no.AC-1001008锛堻/span>

Serum-Free骞茬粏鑳炲喕瀛樻恫锛圏/span>Applied Cell^TM: Cat.no.AC-1001011/1001012锛堻/span>

纾烽吀缂撳啿鐩愭憾娑秉/span>(Phosphate Buffer Saline锛孅/span>PBS)

鎿嶄綔鏂规硶锛堜互涓嬫楠ょ殕搴斿湪鏃犺弻鏉′欢涓嬫搷浣滐級

iPS浜裹/span>璇卞澶氳兘骞秉/span>缁嗚優澶嶈嫃鎿嶄綔锛欬/span>

1.瀹為獙鎿嶄綔姝ラ锛欬/span>

1.1鍩鸿川鑳迭/span>鍖呰鍩瑰吇鏉军/span>锛氬彇鍑裹/span>6瀛旀澘/12瀛旀澘锛屾瘡瀛斿唴鍔犲叆1 mL/0.5 mL鍗崇敤鍨嬪熀璐ㄨ兌锛圏/span>AC-1001007锛堻/span>锛岃交杞绘檭鍔?/span>6瀛旀澘/12瀛旀澘浣垮熀璐ㄨ兌瀹屽叏瑕嗙洊鐨垮簳锛岀疆浜嶞/span>37鈩傸/span>鍩瑰吇绠变腑瀛佃偛1h~2h锛屽疄楠屽墠鎷垮嚭骞剁疆浜庤秴鍑€宸ヤ綔鍙?/span>/鐢熺墿瀹夊叏鏌滀腑瀹ゆ俯涓嬪钩琛狘/span>20min銆傚鏋滄殏鏃朵笉鐢紝鍙敤Parafilm灏佸彛鍚嶞/span>2-8鈩傸/span>鍌ㄥ瓨锛屽苟浜嶞/span>1鍛ㄥ唴浣跨敤銆侟/span>

娉ㄦ剰锛欬/span>鈶燑span style="font-family:妤蜂綋">涓€鏀喕瀛樼殑骞茬粏鑳炵殑鏁伴噺鍦?/span>1脳10⁶cells/mL宸﹀彸锛孅/span>瀵瑰簲6瀛旀澘1瀛旓紱鈶狘/span>鍗崇敤鍨嬪熀璐ㄨ兌瀵规俯搴︽晱鎰烖/span>锛屽嵆鐢ㄥ嵆鎷军/span>锛岀敤瀹屽悗绔嬪嵆鏀惧洖4鈩傸/span>鍐扮淇濆瓨锛孅/span>涓斿嬁鐢ㄦ墜鐩存帴瑙︾鍩鸿川鑳舵恫闈㈡墍鍙婄殑鐡惰韩锛屽奖鍝嶅熀璐ㄨ兌璐ㄩ噺銆侟/span>

1.2鍏圏/span>灏咟/span>2~3mL鐨凕/span>骞茬粏鑳炲煿鍏诲熀鍔犲叆15mL绂诲績绠狘/span>涓?/span>澶囩敤銆侟/span>

1.3瑙ｅ喕锛氬皢浠庢恫姘腑鍙栧嚭鐨勫喕瀛樼蹇€熸蹈鍏?/span>37鈩傸/span>娓╂按涓紝蹇€熸憞鍔紝浣垮叾鍦?/span>1~2min鍐呭揩閫熻В鍐伙紱

娉ㄦ剰锛氱粏鑳炰粠娑叉爱涓嬁鍑虹殑閫熷害瑕佸揩锛屽敖閲忓噺灏戝叾鏆撮湶鍦ㄥ娓╀腑鐨勬椂闂淬€侟/span>

1.4绂诲績锛氬喕瀛樼涓殑鍐诲瓨娑茶В鍐诲悗锛屽皢鍏堕€愭淮鍔燑/span>鍏?/span>鍚湁骞茬粏鑳炲煿鍏诲熀鐨凕/span>15mL绂诲績绠′腑锛屽皢15mL绂诲績绠＄疆浜庝綆閫熺蹇冩満涓厤姣斿钩琛″悗锛孅/span>1000rpm绂诲績3min锛汓/span>

1.5閲嶆偓锛欬/span>绂诲績鍚庡純涓婃竻鍔燑/span>1mL浜哄鑳藉共缁嗚優鍩瑰吇鍩裹/span>瀵瑰共缁嗚優娌夋穩杩涜鍚瑰惛娣峰寑锛屽惞鍚伏/span>3~5娆″乏鍙炽€侟/span>

1.6鎺ョ锛氬惞鍚稿潎鍖€鍚庯紝灏嗗凡缁忓钩琛″ソ鐨凕/span>鍗崇敤鍨嬪熀璐ㄨ兌寮冩帀锛孅/span>灏嗗惞鎵撳潎鍖€鐨勫共缁嗚優鎮恫鍔犲叆鍒?/span>宸茬粡鍖呰濂界殑6瀛旀澘涓紝骞迭/span>琛ュ叏姣忓瓟2mL鍩瑰吇浣撶郴銆侟/span>

1.7鍩瑰吇锛氭帴绉嶅悗鐨凕/span>6瀛旀澘鍙互缃簬鍊掔疆鐩稿樊鏄惧井闀滀笅瑙傚療鎺ョ鐨勫共缁嗚優瀵嗗害浠ュ強缁嗚優鍥㈠潡澶у皬锛屽憟4涓粏鑳炰互涓婄殑鍥㈠潡杈冨悎鏍硷紝姘村钩鍗佸瓧杞昏交鏅冨姩6瀛旀澘/12瀛旀澘浣跨粏鑳炲潎鍖€鍒嗗竷銆傚苟缃簬37鈩傸/span>锛孅/span>5% CO₂鐨勬亽娓╁煿鍏荤鍩瑰吇锛岀2澶╄瀵熺粏鑳炶创澹佹儏鍐碉紱