鎺㈤拡娉曡。鍘熶綋绉戦€氱敤瀹炴椂瀹氶噺PCR妫€娴嬭瘯鍓傜洅

Probe-quantitative Real-time PCR Kit forChlamydiaceae

璐у彿锛欳qt-cea-20 瑙勬牸锛?0涓弽搴擖/span>

璐у彿锛欳qt-cea-50 瑙勬牸锛?0涓弽搴擖/span>

璐у彿锛欳qt-cea-100 瑙勬牸锛?00涓弽搴擖/span>

鍌ㄥ瓨锛?20搴︼紝閬垮厜淇濆瓨锛屾湁鏁堟湡涓€骞淬€傞伩鍏嶅弽澶嶅喕铻嶃€侟/span>

璇曞墏鐩掔壒鐐癸細

1锛?nbsp; 鐗瑰紓鎬ф娴嬭。鍘熶綋/鍡滆。鍘熶綋灞烇紝浠呬笌琛ｅ師浣撶洰锛圏em>Chlamydialesorder 锛夌粏鑿岀殑灏戞暟鍑犵锛圏em>Estrella lausannensis锛學addlia chondrophila锛孲imkania negevensis锛夋湁寰急浜ゅ弶鍙嶅簲锛屼笌琛ｅ師浣撶洰浠ュ鐨勫叾浠栫敓鐗╁熀鍥犵粍鏃犱氦鍙夊弽搴斻€侟/span>

2锛?nbsp; 鐏垫晱搴﹂珮锛屾渶浣庢娴嬫瀬闄愮害56涓熀鍥犵粍/鍙嶅簲銆侟/span>

3锛?nbsp; DNA鑱氬悎閰堕噰鐢ㄧ儹鍚姩鏂瑰紡锛屽彲鎶戝埗闈炵壒寮傛€ф墿澧烇紝闄嶄綆鑳屾櫙鑽у厜銆侟/span>

4锛?nbsp; 甯︽湁闃虫€у鐓ф牱鍝侊紝鍙敤浜庢楠岃瘯鍓傜洅鏈夋晥鎬с€侟/span>

5锛?nbsp; 甯︽湁UDG閰跺拰dUTP锛屽彲闄嶄綆娈嬬暀DNA鐨勬薄鏌撱€侟/span>

琛ｅ師浣撶锛圏em>Chlamydiaceae锛夊睘浜庤。鍘熶綋闂紙Chlamydiaephylum锛夎。鍘熶綋鐩紙Chlamydialesorder锛夛紝浜?966骞寸‘绔嬨€?999骞存牴鎹粎鏈夌殑4涓垚鍛樼殑RNA鍩哄洜搴忓垪锛屽皢鍏跺垎涓轰袱涓灞烇細琛ｅ師浣撳睘锛圏em>Chlamydia锛夊拰鍡滆。鍘熶綋灞?Chlamydophila)銆傞殢鐫€鏇村琛ｅ師浣撶绫荤殑鍙戠幇锛屼袱涓灞炰箣闂寸殑鍖哄埆涓嶅啀鏄庢樉锛屽洜姝?010骞存寮忓皢浜岃€呴噸鏂板悎骞朵负涓€涓灞烇紝浠嶇О涓鸿。鍘熶綋灞烇紙Chlamydia锛夈€傝。鍘熶綋鏈€鏃╁彂鐜颁簬1935骞达紝鐜板湪宸插彂鐜扮殑鏈夌害15绉嶏紝鍏ㄩ儴鏄笓鎬х粏鑳炲唴瀵勭敓锛屽湪浜哄拰鍔ㄧ墿涓彲寮曡捣鐤剧梾銆傜洰鍓嶅凡鍦ㄤ汉銆佸摵涔冲姩鐗┿€侀笩绫诲拰鐖绫诲姩鐗╀腑鍙戠幇琛ｅ師浣撱€傚緢澶氳。鍘熶綋鍙劅鏌撳绉嶅涓伙紝鏈変簺鍒欏叿鏈夊涓荤壒寮傛€э紝鍙劅鏌撲竴绉嶆垨涓€绫诲涓汇€傚彲鎰熸煋浜虹被鐨勮。鍘熶綋鏈夛細娌欑溂琛ｅ師浣擄紙Chlamydia trachomatis锛夛紝鑲虹値琛ｅ師浣擄紙Chlamydophila pneumoniae锛夛紝楣﹂箟鐑。鍘熶綋锛圏em>Chlamydophila psittaci锛夛紝娴佷骇琛ｅ師浣擄紙Chlamydophila abortus锛夌瓑銆傝。鍘熶綋涓昏渚佃鍛煎惛閬撱€佽儍鑲犻亾鎴栫敓娈栫郴缁熺殑绮樿啘锛屾劅鏌撲綅鐐瑰拰涓ラ噸绋嬪害涓庤。鍘熶綋绉嶇被鍜屽涓婚兘鏈夊叧绯汇€傛劅鏌撳悗鍦ㄥ鏁板姩鐗╀腑浼氬紩璧风粨鑶滅値鍜岄蓟绮樿啘鐐庯紝鎴栬€呬镜琚敓娈栫郴缁熷鑷存祦浜у拰涓嶈偛锛屼絾鏈夋椂骞朵笉琛ㄧ幇鍑虹棁鐘躲€傞笩绫绘劅鏌撻功楣夌儹琛ｅ師浣撳悗甯稿浜庝簹涓村簥鐘舵€侊紝鍦ㄥ帇鍔涗笅鍙兘鍑虹幇涓村簥鐥囩姸锛岃〃鐜颁负閲嶅害鑲虹値鍜屽櫒瀹樿“绔€備汉绫诲彲鍦ㄥ吇楦熻繃绋嬩腑琚劅鏌擄紝浜х敓绫讳技娴佹劅鐨勭棁鐘讹紝甯搁€犳垚璇瘖瀵艰嚧涓ラ噸鍚庢灉銆侟/span>

琛ｅ師浣撳彲浠ラ€氳繃鍩瑰吇娉曞拰琛€娓呭鏂规硶杩涜妫€娴嬨€傜敱浜庤。鍘熶綋鏄笓鎬х粏鑳炲唴瀵勭敓锛屽洜姝ゅ繀椤诲湪娲荤粍缁囨垨缁嗚優鍐呰繘琛屽煿鍏伙紝鎿嶄綔澶嶆潅锛岃€楁椂锛岀伒鏁忓害浣庛€傝娓呭鏂规硶鍚屾牱鐏垫晱搴﹁緝浣庯紝涓斿彧鑳藉湪宸叉劅鏌撲竴娈垫椂闂翠箣鍚庢墠鑳芥娴嬨€侾CR娉曞叿鏈夌伒鏁忓害楂樸€佺壒寮傛€у己鐨勪紭鐐癸紝妫€娴嬮€熷害蹇紝鍙渶涓や笁涓皬鏃跺嵆鍙畬鎴愶紝鍙互鏈夋晥寮ヨˉ涓婅堪涓ょ鏂规硶鐨勪笉瓒炽€侟/span>

鏈瘯鍓傜洅閽堝16S-23S rRNA鍩哄洜鍖洪棿搴忓垪璁捐鐗瑰紓鎬у紩鐗╁拰鎺㈤拡锛屽彲浠ョ壒寮傛€ц瘑鍒悇绉嶈。鍘熶綋/鍡滆。鍘熶綋锛岀粡BLAST楠岃瘉锛屼粎涓庤。鍘熶綋鐩紙Chlamydialesorder 锛夌粏鑿岀殑灏戞暟鍑犵锛圏em>Estrella lausannensis锛孅em>Waddlia chondrophila锛孅em>Simkania negevensis锛夋湁寰急浜ゅ弶鍙嶅簲锛屼笌鍏朵粬鐢熺墿鍩哄洜缁勬病鏈変氦鍙夊弽搴斻€傚湪楂樻祿搴︾殑鎯呭喌涓嬶紝杩欏嚑绉嶈。鍘熶綋鐩粏鑿屾湁鍙兘浜х敓鐗瑰紓鎬т俊鍙枫€傛湰璇曞墏鐩掓娴嬩簡145涓敓鐗╂牱鍝侊紝鍖呮嫭缁撹啘鎷瓙銆侀蓟鑵旀嫮瀛愩€佸ザ銆佹柊椴滅粍缁囥€佷綋娑插拰绮究绛夊悇绉嶇被鍨嬶紝鑾峰緱125涓槼鎬э紝涓嶥NA寰偣闃垫硶妫€娴嬬粨鏋滃熀鏈竴鑷达紝閰嶅悎娴嬪簭缁撴灉璇佸疄鍏朵腑鍖呭惈6绉嶈。鍘熶綋锛屽寘鎷竴绉嶆湭鎶ラ亾杩囩殑鏂拌。鍘熶綋鐗╃銆侟/span>

鏈骇鍝佷粎渚涚爺绌朵娇鐢ㄣ€侟/span>