50051-M07H-20 ?g |Legumain閲嶇粍铔嬬櫧|Recombinant Mouse Legumain/ LGMN Protein (His Tag)锛堜骇鍝佽鏄庯級
铔嬬櫧鍚嶇О锛欬/strong>Mouse Legumain/ LGMN Protein (His Tag)
铔嬬櫧鏋勫缓锛欬/strong>A DNA sequence encoding the extracellular domain (Val 18-Tyr 435) of mouse LGMN (NP_035305.1) precursor was expressed with a C-terminal polyhistidine tag.
琛ㄨ揪瀹夸富锛欬/strong>HEK293 Cells
铔嬬櫧绾害锛欬/strong>鈮 75 % as determined by SDS-PAGE. 鈮 95 % as determined by SEC-HPLC.
铔嬬櫧娲绘€э細Measured by its ability to cleave the fluorogenic peptide substrate, N-carbobenzyloxy-Ala-Ala-Asn-7-amido-4-methyl coumarin(Z-AAN-AMC). The specific activity is > 350 pmoles/min/渭g. (Activation description: The enzyme achieves its activity under acidic pH)
铔嬬櫧鍐呮瘨绱狅細< 1.0 EU per 渭g of the protein as determined by the LAL method
棰勬祴N绔細Thr 22
铔嬬櫧鍒嗗瓙閲忥細The secreted recombinant mouse LGMN consists of 434 amino acids and has a calculated molecular mass of 49.8 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 55 kDa protein in SDS-PAGE under reducing conditions.
铔嬬櫧NP鍙凤細NP_035305.1
铔嬬櫧姘ㄥ熀閰稿簭鍒楋細Val18-Tyr435
铔嬬櫧鏍囩锛欬/strong>N-His