璐?/span>鍙饵/span>	瑙勬牸锛坢l锛堻/span>	鐩綍浠凤紙鍏冿級
T11007	0.5	1820
T11008	1.0	3430
T11009	2.0	5480

浜у搧绠€浠婞/span>

StarvioPMsiRNA鍘熶唬缁嗚優杞煋璇曞墏鏄垜鍏徃鐮斿彂鍥㈤槦鍦⊿tarvio缁嗚優鏍猻iRNA杞煋璇曞墏鐨勫熀纭€涓婂姞浠ユ敼杩涚爺鍙戞垚鍔熺殑涓撻棬鐢ㄤ簬鍘熶唬缁嗚優灏忔牳閰歌浆鏌撶殑璇曞墏銆係tarvioPM鍙敤鏉ヨ浆鏌搒iRNA銆乵iRNA mimics銆乵iRNA inhibitor绛?00bp浠ュ唴鐨勫皬鍒嗗瓙RNA锛屽彲杞煋缁濆ぇ澶氭暟鍘熶唬缁嗚優銆傜洰鍓嶏紝鏃犺鍥藉杩樻槸鍥藉唴锛屽師浠ｇ粏鑳炵殑灏忔牳閰歌浆鏌撻兘鏄釜鐑偣闅鹃锛屽競鍦鸿繕缂轰箯鐪熸鏈夋晥鐨勫晢鍝佸寲鐨勫師浠ｇ粏鑳炲皬鏍搁吀杞煋璇曞墏銆係tarvioPM鑳藉楂樻晥杞煋缁濆ぇ澶氭暟鍘熶唬缁嗚優锛岃幏寰楁瘮杈冪悊鎯崇殑鍩哄洜鏁查櫎鏁堟灉銆傜敋鑷冲涓€浜涙椿浣撳瘎鐢熻櫕骞艰櫕锛孲tarvioPM涔熻兘鑾峰緱杈冧负鐞嗘兂鐨勮浆鏌撶粨鏋溿€傚浜庡ぇ澶氭暟鍘熶唬缁嗚優锛孲tarvioPM鐨勭粏鑳炶浆鏌撻槼鎬х巼閮藉湪85%浠ヤ笂锛岃€岃浆鏌撶粏鑳炴浜＄巼涓嶅埌10%銆係tarvioPM鐨勪娇鐢ㄤ篃鏋佸叾绠€渚匡紝鍏堝皢siRNA涓嶴tarvioPM瀹ゆ俯娣峰悎锛屽啀灏唖iRNA-StarvioPM娣峰悎鐗╃洿鎺ュ姞鍏ュ惈鍩瑰吇鍩虹殑缁嗚優锛岃娓呭杞煋鏁堟灉娌℃湁褰卞搷锛屼笉蹇呭埢鎰忔坊鍔犳垨鏇存崲鍩瑰吇娑层€侟/p>浜у搧鐗圭偣

路 浼樿秺鐨勫師浠ｇ粏鑳炶浆鏌撴€ц兘锛氬彲楂樻晥杞煋缁濆ぇ澶氭暟鍘熶唬缁嗚優锛屽熀鍥犳暡闄ゆ晥鏋滄槑鏄撅紝鍘熶唬鑲濈粏鑳濴amin A/C 鍩哄洜鏁查櫎鏁堢巼鍦?0%浠ヤ笂锛汓/p>

路 鏋佷綆鐨勭粏鑳炴瘨鎬э細杞煋缁嗚優姝讳骸鐜囦笉鍒?0%锛屽ぇ澶ч檷浣庝簡鍥犵粏鑳炴瘨鎬у瀹為獙缁撴灉鐨勫奖鍝嶏紱

路 杞煋缁嗚優鑼冨洿骞匡紝缁濆ぇ澶氭暟鍘熶唬缁嗚優閮借兘鑾峰緱姣旇緝鐞嗘兂鐨勮浆鏌撶粨鏋溿€侟p style="box-sizing: border-box; margin-top: 0px; margin-bottom: 0px; padding: 0px; text-align: center;">

Fig.1. Superior knockdown with StarvioPM transfection reagent on difficult transfection primary cells.Differentprimarycells were transfected with scrambled or Lamin A/C siRNA (final concentration: 10nM) using StarvioPM.In this and all the following figures, all the cells are harvested 48 hours after transfection and Lamin A/C knockdown was measured by qRT-PCR.

Fig.2. StarvioPM transfection reagent offers high level of gene knockdown with low concentration of siRNA. Lamin A/C siRNA-StarvioPM transfection complexes were prepared in 96-well plates,and then bovine chondrocytes were added to each well.

閫傜敤缁嗚優

Clone 9锛堝ぇ榧犺倽缁嗚優锛夛紝HUVEC锛堜汉鑴愰潤鑴夊師浠ｅ唴鐨粏鑳烇級锛屽師浠ｈ绠″钩婊戣倢缁嗚優锛屽ぇ榧犲師浠ｅ績鑲岀粏鑳烇紝Primary Human Fibroblasts锛堜汉鍘熶唬鎴愮氦缁寸粏鑳烇級锛屽ぇ榧犻楂撻棿鍏呰川骞茬粏鑳烇紝MSC锛堥棿鍏呰川骞茬粏鑳烇級锛孊E(2)C锛堜汉绁炵粡姣嶇粏鑳炵槫缁嗚優锛夛紝MRC-5锛堜汉鑳氳儙鎴愮氦缁寸粏鑳烇級锛孨IH3T3锛堝皬榧犺儦鑳庢垚绾ょ淮缁嗚優锛夛紝B16F10锛堝皬榧犻粦鑹茬礌鐦ら珮杞Щ缁嗚優锛夛紝BNL.CL2锛堝皬榧犺儦鑳庤倽缁嗚優锛夛紝C6锛堝ぇ榧犵缁忚兌璐ㄧ槫缁嗚優锛夛紝绁炵粡鍏冪粏鑳炵瓑銆侟/p>

鐩稿叧鏂囩尞

1. Basic Research in Cardiology(2021). HIMF deletion ameliorates acute myocardial ischemic injury by promoting macrophage transformation to reparative subtype. (IF:11.981)

2. NUCLEIC ACIDS RESEARCH(2021). XPD localizes in mitochondria and protects the mitochondrial genome from oxidative DNA damage. (IF:11.24 )

3. MYO1F regulates antifungal immunity by regulating acetylation of microtubules. (IF:11.205)

4. Cellular & Molecular Immunology(2021). The miR-628-5p Facilitates Enterovirus 71 Infection by Suppressing TRAF3-Mediated p65 and IRF3 Signaling. (IF:8.484)

5. Levobupivacaine Induces Ferroptosis by miR-489-3p/SLC7A11 Signaling in Gastric Cancer. (IF:5.81)

6. Proteome Analysis in PAM Cells Reveals That African Swine Fever Virus Can Regulate the Level of Intracellular Polyamines to Facilitate Its Own Replication through ARG1. (IF:5.046)

7. AGING-US(2021). Extracellular vesicles from anoxia preconditioned mesenchymal stem cells alleviate myocardial ischemia/reperfusion injury. (IF:4.909 )

8. International Journal of Biological Sciences(2021). DGCR5 Promotes Gallbladder Cancer by Sponging MiR-3619-5p via MEK/ERK1/2 and JNK/p38 MAPK Pathways. (IF:4.773)

9. International Journal of Biological Sciences(2021). Rac GTPase activating protein 1 promotes gallbladder cancer via binding DNA ligase 3 to reduce apoptosis. (IF:4.773) DOI: 10.7150/ijbs.58857

10. International Journal of Biological Sciences(2021). ESRRA promotes gastric cancer development by regulating the CDC25C/CDK1/CyclinB1 pathway via DSN1. (IF:4.773) DOI:10.7150/ijbs.57623

11. International Journal of Biological Sciences(2021). KIF11 promotes cell proliferation via ERBB2/PI3K/AKT signaling pathway in gallbladder cancer. (IF:4.773 )

12. International Journal of Biological Sciences(2021). DGCR5 is activated by PAX5 and promotes pancreatic cancer via targeting miR-3163/TOP2A and activating Wnt/尾-catenin pathway. (IF:4.773 ) DOI: 10.7150/ijbs.55636

13. Journal of Cellular and Molecular Medicine(2021). Down鈥 egulation of RCC1 sensitizes immunotherapy by up鈥 egulating PD鈥怢1 via p27kip1/CDK4 axis in non鈥恠mall cell lung cancer. (IF:4.438)

14. scientific reports(2021). Clinical significance of Annexin A2 e­xpression in oral squamous cell carcinoma and its influence on cell proliferation, migration and invasion. (IF:4.149)

15. Journal of Cancer(2021). LncRNA LOXL1-AS1 promotes esophageal squamous cell carcinoma progression by targeting DESC1. (IF:3.565)

16. Environmental Geochemistry and Health(2021). Inorganic arsenic influences cell apoptosis by regulating the e­xpression of MEG3 gene. (IF:3.472 )

17. Bta-miR-223 Targeting CBLB Contributes to Resistance to Staphylococcus aureus Mastitis Through the PI3K/AKT/NF-魏B Pathway. (IF:3.412)

18. Molecular Human Reproduction(2021). Excess fibronectin 1 participates in pathogenesis of pre-eclampsia by promoting apoptosis and autophagy in vascular endothelial cells. (IF:3.469)

19. American Journal of Translational Research(2021). Irg1-itaconate axis protects against acute kidney injury via activation of Nrf2. (IF:3.375 )

20. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH(2021). Arsenic exposure increased e­xpression of HOTAIR and LincRNA-p21 in vivo and vitro. (IF:3.33)