Biotin-16-dCTP,orb64049
| 鍨嬪彿: |
orb64049 |
| 鍝佺墝: |
biorbyt |
| 鍙傝€冧环鏍机 |
6539鍏?mL |
| 绾害鍚噺: |
鈮 95% (HPLC) |
| 绛夌骇瑙勬牸: |
鐗圭骇 |
| 鍖呰: |
1ml |
| 浜よ揣鏈烔 |
2~4鍛?/td> |
| 浜у湴: |
UK |
| 鍒嗗瓙寮廁 |
C32H51N8O17P3S |
| 鍒嗗瓙閲廁 |
Theoretical MW: 944.78 g/mol (free acid); Detected |
浜у搧鍚嶇О
Biotin-16-dCTP(biotin)
浜у搧浠嬬粛
| Catalog Number |
orb64049 |
| Category |
Tools |
| Description |
Biotin-16-dCTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dCTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal substrate properties and thus labeling efficiency as well as an efficient detec-tion of the Biotin moiety is ensured by a 16-atom linker attached to the C5 position of cytidine. Recommended Biotin-16-dCTP/dCTP ratio for PCR: 50% Biotin-16-dCTP/50% dCTP. |
| Concentration |
1.0 mM-1.1 mM |
| Form/Appearance |
filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 卤 0.5 |
| Purity |
鈮 95% (HPLC) |
| MW |
Theoretical MW: 944.78 g/mol (free acid); Detected MW: 944.23 g/mol (free acid) |
| SMILES |
C1[C@H]2[C@@H]([C@@H](S1)CCCCC(=O)NCCCCCC(=O)NCCCC(=O)NCC#Cc1cn(c(=O)nc1N)[C@@H]1O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)C1)NC(=O)N2 |
| Formula |
C32H51N8O17P3S |
| Storage |
store at -20 掳C |
| Note |
For research use only |
| Application notes |
Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase I; Primer Extension with Klenow exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase.
Spectroscopic Propertie: 位max 294 nm, 蔚 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5). |
| Expiration Date |
12 months from date of receipt. |
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