閲嶇粍浜哄啝鐘剁梾姣?HCoV-HKU1)(isolate N1)Spike/S1铔嬬櫧(S1 Subunit,His鏍囩)锛堜骇鍝佽鏄庯級
铔嬬櫧鍚嶇О锛欬/strong>Coronavirus spike铔嬬櫧, Coronavirus spike protein
铔嬬櫧鏋勫缓锛欬/strong>A DNA sequence encoding the human coronavirus HKU1 (isolate N1) (HCoV-HKU1) spike protein (S1 Subunit) (YP_173238.1) (Met 1-Arg 760) was expressed with a polyhistidine tag at the C-terminus.
琛ㄨ揪瀹夸富锛欬/strong>HEK293 Cells
铔嬬櫧绾害锛欬/strong>> 90 % as determined by SDS-PAGE
铔嬬櫧娲绘€э細0
铔嬬櫧鍐呮瘨绱狅細< 1.0 EU per 渭g of the protein as determined by the LAL method
棰勬祴N绔細Gly 16
铔嬬櫧鍒嗗瓙閲忥細The recombinant human coronavirus HKU1 (isolate N1) (HCoV-HKU1) spike protein (S1 Subunit, His Tag) consists of 756 amino acids with a predicted molecular mass of 85.8 kDa. As a result of high glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
铔嬬櫧NP鍙凤細YP_173238.1
铔嬬櫧姘ㄥ熀閰稿簭鍒楋細Met1-Arg760
铔嬬櫧鏍囩锛欬/strong>C-His
铔嬬櫧淇濆瓨鏉′欢锛欬/strong>Store it under sterile conditions at -20鈩 to -80鈩? It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.