1銆佸紩 瑷€

纾鸿兒(Sulfonamides锛孲As)鏄竴绫诲箍璋辨姉鑿岃嵂鐗╋紝骞挎硾搴旂敤浜庢按浜у吇娈栦笟鍜岀暅鐗т笟锛屾槸棰勯槻鍜屾不鐤楃粏鑿屾劅鏌撴€х柧鐥呯殑鍖栧鑽墿銆傜：鑳虹被鑽墿杩樼敤浜庨┈銆佺墰鐨勪紶鏌撴€ц剳鑶滅値锛岀緤涓嬬棦銆佺尓涓嬬棦鍜屽紦褰綋鐥咃紝骞朵笖瀵逛簬绂界被鐞冭櫕鐥呭叿鏈夎緝濂界枟鏁堛€傜敱浜庡吇娈栦笟涓：鑳虹被鑽墿鐨勪娇鐢ㄥ鑷翠簡鍔ㄧ墿婧愭€ч鐗╀腑娈嬬暀锛屼粠鑰屽嵄瀹充汉浣撳仴搴枫€傛垜鍥藉拰娆х洘鍧囪瀹氫簡纾鸿兒鍦ㄥ姩鐗╃粍缁囦腑鐨勬渶楂樻畫鐣欓檺閲忋€傚姩鐗╃粍缁囦腑纾鸿兒鐨勬娴嬫柟娉曚富瑕佹湁娑茬浉鑹茶氨涓茶仈璐ㄨ氨娉曪紝娑茬浉鑹茶氨绱妫€娴嬫硶锛屾恫鐩歌壊璋辫崸鍏夋娴嬫硶銆傜：鑳虹被鑽墿鑷韩涓嶅叿鏈夎崸鍏夋椿鎬э紝閲囩敤鑽у厜妫€娴嬫硶鏃堕€氬父闇€瑕佽繘琛屾煴鍓嶆垨鏌卞悗鍖栧璇曞墏琛嶇敓鎵嶈兘杩涜妫€娴嬨€傜爺绌跺彂鐜帮紝纾洪叞鑳哄熀鏄厜鍖栧娲绘€у熀鍥紝鍦ㄧ传澶栧厜鐓у皠涓嬩細鍙戠敓鍏夊寲瀛﹀弽搴旓紝浜х墿鍏锋湁鑽у厜鎬ц川锛岄€氳繃鑽у厜鍏夊害娉曞彲浠ュ鍏惰繘琛屾娴嬨€傛渶杩戠爺鍒跺嚭涓€绉嶅厜鍖栧鍦ㄧ嚎琛嶇敓鍣紝搴旂敤浜庨珮鏁堟恫鐩歌壊璋卞悗锛屽彲浠ヨ繘琛屽湪绾垮厜鍖栧琛嶇敓浣垮垎瀛愪骇鐢熻崸鍏夋椿鎬э紝鍒╃敤鑽у厜妫€娴嬪櫒鍙互鏈夋晥鍦版娴嬨€傜浉姣斾簬鏌卞墠鎴栨煴鍚庡寲瀛﹁瘯鍓傝鐢熸硶锛屽厜鍖栧琛嶇敓涓嶉渶瑕侀澶栫殑琛嶇敓鍖栬瘯鍓傦紝涔熶笉闇€瑕佹煴鍚庤鐢熷弽搴旂殑璇曞墏娉碉紝鍑忓皯浜嗕汉宸ユ搷浣滐紝鍚屾椂涔熼伩鍏嶈鐢熷寲杩囩▼涓汉涓烘搷浣滃洜绱犲甫鏉ョ殑璇樊銆傚厜鍖栧琛嶇敓涓嶅紩鍏ュ叾浠栨潅璐紝鍑忓皯浜嗕汉浣撲笌鏈夋瘨璇曞墏鐨勬帴瑙﹀拰鏇濋湶锛屾洿鍔犵豢鑹层€佺幆淇濄€佺畝渚裤€佸揩鎹枫€傚埌鐩墠涓烘锛屽叧浜庡厜鍖栧鏌卞悗琛嶇敓楂樻晥娑茬浉鑹茶氨鑽у厜娉曞湪纾鸿兒绫昏嵂鐗╂娴嬩笂鐨勫簲鐢ㄥ皻鏈鎶ラ亾銆傛湰瀹為獙绯荤粺鐮旂┒浜嗗厜鍖栧琛嶇敓鐨勫疄楠屾潯浠讹紝寤虹珛浜嗙畝渚匡紝鍙潬锛岀伒鏁忓害楂樼殑鍔ㄧ墿缁勭粐涓：鑳鸿嵂鐗╂畫鐣欑殑妫€娴嬫柟娉曘€侟/span>

2銆佸疄楠岄儴鍒咟/strong>

2.1 浠櫒涓庤瘯鍓?nbsp;

Agilent 1260楂樻晥娑茬浉鑹茶氨浠厤鑽у厜妫€娴嬪櫒(缇庡浗Agilent鍏徃)锛涘厜鍖栧琛嶇敓鍣?鐢辩传澶栫伅(11W)銆佽仛鍥涙盁涔欑儻鍙嶅簲绠?25m 脳 0.25mm)缁勬垚锛屽寳浜崕瀹夐害绉戝叕鍙?锛涢珮閫熷寑娴嗘満(寰峰浗IKA鍏徃)锛涢珮閫熺蹇冩満(缇庡浗Thermo鍏徃浜у搧)锛涙棆杞捀鍙戜华(寰峰浗IKA鍏徃)锛涙爱姘斿惞骞蹭华(澶╂触甯傛亽濂ョ鎶€鏈夐檺鍏徃)锛涙丁鏃嬫贩鍚堝櫒(澶粨甯傚崕鍒╄揪瀹為獙璁惧鏈夐檺鍏徃)銆侟/span>

鐢查唶銆佷箼鑵?鑹茶氨绾э紝寰峰浗Merck鍏徃)锛涚敳閰搞€佹宸辩兎(鑹茶氨绾э紝缇庡浗TEDIA鍏徃)锛涘疄楠岀敤姘翠负Milli-Q瓒呯函姘淬€侟/span>

鏍囧噯鐗╄川锛氱：鑳哄槯鍟?绾害鈮?9.5%)锛岀：鑳哄櫥鍞?绾害鈮?9.5%)锛岀：鑳虹敳鍩哄槯鍟?绾害鈮?9.2%)锛岀：鑳哄悺鍟?绾害鈮?9.0%)锛岀：鑳轰簩鐢插熀鍢у暥(绾害鈮?9.0%)锛岀：鑳哄鐢叉哀鍢у暥(绾害鈮?8.0%)锛岀：鑳烘隘鍝掑棯(绾害鈮?9.0%)锛岀：鑳虹敳姘у搾鍡?绾害鈮?9.2%)锛岀：鑳轰簩鐢插紓鍣佸攽(绾害鈮?8.0%)锛岀：鑳鸿嫰鐢查叞(绾害鈮?9.0%)锛岀：鑳哄柟鍣佸晧(绾害鈮?8.0% )銆傛爣鍑嗙墿璐ㄥ潎璐簬寰峰浗Dr.Ehrenstorfer GmbH鍏徃銆侟/span>

鏍囧噯婧舵恫鐨勯厤鍒讹細鍒嗗埆鍑嗙‘绉板彇10.0mg涓婅堪鏍囧噯鍝佷簬10.0mL瀹归噺鐡朵腑锛岀敤鐢查唶瀹氬锛岄厤鍒舵垚1.0g/L鐨勫偍澶囨恫锛屼簬-18鈩冨偍瀛樸€傚垎鍒惛鍙?.0g/L鐨勬爣鍑嗘憾娑?0渭L浜?0.0mL瀹归噺鐡朵腑锛岀敤鐢查唶瀹氬锛岄厤鎴?.0mg/L娣峰悎鏍囧噯婧舵恫锛岀敤鏃剁敤娴佸姩鐩哥█閲婃垚涓€绯诲垪娴撳害鐨勬爣鍑嗘憾娑层€侟/span>

2.2 鑹茶氨鏉′欢

Eclipse Plus C18娑茬浉鑹茶氨鏌?250mm 脳 4.6mm锛?.0渭m锛 Agilent鍏徃)锛涙煴娓╋細35鈩冿紱杩涙牱閲?0渭L锛涙祦鍔ㄧ浉A涓虹敳閱囷紝B涓轰箼鑵堬紝C涓烘按(鍧囧惈0.2%(V/V)涔欓吀)锛屾搴︽礂鑴憋細0.0锝?2.0min锛?0% A锛?% B锛?5% C锛?2.0锝?8.0min锛?0% A锛?%锝?2% B锛?5%锝?8% C锛?0.00锝?3.00min锛?0% A锛?2% B锛?8% C锛?3.00锝?3.10min锛?0% A锛?2%锝?% B锛?8%锝?5% C銆傛祦閫?.0mL/min銆傚厜鍖栧琛嶇敓鍣ㄧ传澶栫伅鐓у皠娉㈤暱254nm锛岀収灏勬椂闂翠负1min銆傝崸鍏夋娴嬪櫒锛氭縺鍙戞尝闀?48nm锛屽彂灏勬尝闀?50nm锛涙縺鍙戞尝闀?48nm锛屽彂灏勬尝闀?12nm銆侟/span>

2.3 鏍峰搧鍓嶅鐞咟/strong>

绉板彇鑲夋牱5.0g(绮剧‘鍒?.01g)浜?0.0mL绂诲績绠′腑锛屽姞鍏?g鏃犳按Na2SO4锛?0mL涔欒厛锛岀敤楂橀€熷寑娴嗘満浠?2,000r/min鍖€娴?min锛屼互8,000r/min绂诲績10min锛屼笂娓呮恫杞Щ鑷?00mL鐑х摱涓紝娈嬬暀鐗╃敤20mL涔欒厛閲嶅鎻愬彇涓€娆★紝鍚堝苟涓婃竻娑层€傜敤鏃嬭浆钂稿彂鍣ㄥ湪40鈩冿紝17kPa璐熷帇涓嬭捀鑷宠繎骞诧紝鐢ㄦ爱姘斿惞骞叉畫浣欐恫锛屽姞鍏?.0mL娴佸姩鐩稿拰1.0mL姝ｅ繁鐑峰厖鍒嗘憾瑙ｏ紝杞Щ鑷?.0mL绂诲績绠′腑锛屼互8,000r/min绂诲績10min锛屽純鍘讳笂灞傛宸辩兎锛屽啀娆″姞鍏?.5mL姝ｅ繁鐑锋丁鏃?.0min锛屼互8,000r/min绂诲績10min锛屽純鍘讳笂灞傛宸辩兎锛屼笅灞傛竻娑茶繃0.22渭m婊よ啘锛屾护娑蹭緵HPLC鍒嗘瀽銆侟/span>

3銆佺粨鏋滀笌璁ㄨ

3.1 鍦ㄧ嚎鍏夊寲瀛﹁鐢熷弽搴擖/strong>

纾鸿兒绫昏嵂鐗╄嚜韬棤鑽у厜鐗规€э紝涓嶈兘閲囩敤鑽у厜妫€娴嬪櫒鐩存帴杩涜妫€娴嬨€傜：閰拌兒鍩烘槸鍏夊寲瀛︽椿鎬у熀鍥紝鍦ㄧ传澶栧厜鐓у皠涓嬩細鍙戠敓鍏夊寲瀛﹀弽搴旓紝鍏夊寲瀛﹀弽搴旂殑浜х墿鍙兘涓?,4'-浜屾皑鍩轰簩鑻厷銆佽嫰鑳哄拰瀵规皑鍩鸿嫰纾洪吀鐨勬贩鍚堜骇鐗╋紝浜х墿鍏锋湁鑽у厜鎬ц川銆傚洜姝ゅ彲浠ュ湪鏌卞悗鍔犺鍏夊寲瀛﹁鐢熷櫒锛屽纾鸿兒鑽墿杩涜鍦ㄧ嚎鍏夊寲瀛﹁鐢熷寲锛岀劧鍚庨噰鐢ㄨ崸鍏夋娴嬪櫒杩涜妫€娴嬨€傜：鑳虹粡杩囩传澶栧厜璇卞鍚庡寲瀛﹂敭鍙戠敓鍙樺寲浜х敓鐨勮崸鍏夋€э紝鍏跺己搴﹀彈鍒嗗瓙缁撴瀯鐨勫奖鍝嶈緝澶с€傚洜涓轰笉鍚岀殑鍙栦唬鍩哄洟瀵瑰厜鍖栧鍙嶅簲鐨勫奖鍝嶄笉鍚岋紝鎵€浠ヤ笉鍚屽彇浠ｅ熀鐨勭：鑳鸿嵂鐗╃殑鑽у厜寮哄害涔熶笉涓€鏍凤紝婵€鍙戞尝闀垮拰鍙戝皠娉㈤暱涔熷瓨鍦ㄧ潃宸紓锛岃繖涓€鐐逛笌鏌卞墠鎴栨煴鍚庡寲瀛﹁瘯鍓傝鐢熶笉鍚屻€傚父瑙勭殑鏌卞墠鎴栨煴鍚庡寲瀛﹁瘯鍓傝鐢熶竴鑸槸浣跨洰鏍囩墿琛嶇敓鍖栦笂涓€涓崸鍏夊熀鍥紝鍏舵娴嬬殑婵€鍙戞尝闀垮拰鍙戝皠娉㈤暱涓€鑸槸璇ヨ崸鍏夊熀鍥㈢殑婵€鍙戞尝闀垮拰鍙戝皠娉㈤暱銆傚皢纾鸿兒绫昏嵂鐗╂爣鍑嗘憾娑插湪绾垮厜鍖栧琛嶇敓鍖栧悗杩涜鑽у厜鍏夎氨鎵弿锛屽悇纾鸿兒鑽墿鐨勬渶浣虫縺鍙戞尝闀垮湪235锝?60nm涔嬮棿锛屾渶浣冲彂灏勬尝闀垮湪320锝?50nm涔嬮棿锛岀患鍚堣€冭檻锛岀‘瀹氱：鑳哄櫥鍞戙€佺：鑳虹敳鍩哄槯鍟躲€佺：鑳轰簩鐢插槯鍟躲€佺：鑳轰簩鐢插紓鍣佸攽鍜岀：鑳鸿嫰鐢查叞鐨勬縺鍙戞尝闀夸负248nm锛屽彂灏勬尝闀夸负350nm锛涚：鑳哄槯鍟躲€佺：鑳哄悺鍟躲€佺：鑳烘隘鍝掑棯銆佺：鑳虹敳姘у搾鍡€佺：鑳哄鐢叉哀鍢у暥鍜岀：鑳哄柟鍣佸晧鐨勬縺鍙戞尝闀夸负248nm锛屽彂灏勬尝闀夸负412nm銆?nbsp;

11绉嶇：鑳鸿嵂鐗╃殑鏍囧噯鑹茶氨宄扮粡鍏夊寲瀛﹁鐢熷櫒琛嶇敓鍓嶅悗鐨勬晥鏋滄瘮杈冭鍥?銆?1绉嶇：鑳鸿嵂鐗╃殑鏍囧噯婧舵恫鍦ㄥ厜鍖栧琛嶇敓鍣ㄦ湭寮€鍚椂锛屽悇纾鸿兒鑽墿鍑犱箮娌℃湁鑽у厜鍝嶅簲淇″彿(鍥?A) 锛涘厜鍖栧琛嶇敓鍣ㄥ紑鍚悗锛岃崸鍏夊ぇ骞呭寮?鍥?B)銆侟/span>

3.2 楂樻晥娑茬浉鑹茶氨鏉′欢鐨勪紭鍖朁/strong>

鍙傝€冪：鑳烘娴嬬殑鐩稿叧鏂囩尞锛屼娇鐢ㄧ敳閱囥€佷箼鑵堛€佷箼閰告憾娑蹭笁鐩镐綔涓烘祦鍔ㄧ浉锛屽姣斾簡鐢查唶銆佷箼鑵堜负鏈夋満鐩镐笌鍚笉鍚屾祿搴︾殑涔欓吀婧舵恫缁勬垚鐨勬祦鍔ㄧ浉瀵圭：鑳鸿嵂鐗╁垎绂荤殑褰卞搷銆傚疄楠屽彂鐜帮紝涓嶅惈閰哥殑娴佸姩鐩革紝纾鸿兒鐨勮嵂鐗╃殑鑹茶氨宄板舰鍑虹幇鎷栧熬锛岄儴鍒嗙粍鍒嗛噸鍙犮€傚姞鍏ヤ箼閰稿悗锛岃壊璋卞嘲褰㈠绉帮紝鍒嗙鏁堟灉濂姐€傝€冨療浜嗕笉鍚屾祿搴︿箼閰稿鑹茶氨宄板舰鐨勫奖鍝嶏紝鍙戠幇0.2%涔欓吀鏃㈣兘淇濊瘉杈冨ソ鐨勫垎绂绘晥鏋滐紝鍚屾椂涔熶笉褰卞搷鑹茶氨鏌辩殑浣跨敤瀵垮懡銆傜敱浜庝箼鑵堢殑娲楄劚鑳藉姏杈冨己锛岀：鑳鸿嵂鐗╁湪鑹茶氨鏌变笂鐨勪繚鐣欐椂闂寸煭锛岄儴鍒嗙：鑳哄垎绂诲害杈冨樊锛岀敳閱囩殑娲楄劚鑳藉姏杈冨急锛屽垎绂诲害濂斤紝浣嗘槸纾鸿兒鑽墿鐨勪繚鐣欐椂闂磋緝闀匡紝涓斿嘲鍨嬭緝鎵佸钩銆傞噰鐢ㄥ悎閫傜殑鐢查唶銆佷箼鑵堛€佷箼閰稿彲寰楀埌杈冨ソ鐨勫垎绂诲害鍜岃緝蹇殑鍒嗙鏁堢巼銆備紭鍖栧悗鐨勮壊璋卞垎绂绘潯浠惰2.2鑺傦紝11绉嶇：鑳虹殑鏍囧噯鑹茶氨鍥捐鍥?銆侟/span>

鍥? 鍏夊寲瀛﹀弽搴斿墠(A)鍚?B)11绉嶇：鑳虹殑楂樻晥娑茬浉鑹茶氨鍥近/span>

Fig.1 HPLC chromatograms of 11 sulfonamides before (A) and after (B) on-line photochemical derivatization 1: Sulfadiazine, 2: Sulfathiazole, 3: Sulfamerazine, 4: Sulfapyridine, 5: Sulfachloropyridazine, 6: Sulfadoxine, 7: Sulfamethoxy-pyridazine, 8: Sulfameter, 9: Sulfisoxazole, 10: Sulfabenzamide,11: Sulfaquinoxaline.

3.3 鍓嶅鐞嗘柟娉曠殑寤虹珛

姣旇緝浜嗙敳閱囥€佷箼鑵堛€佷箼閰镐箼閰殑鎻愬彇鏁堟灉銆傜敳閱囩殑鎻愬彇鏁堢巼杈冧綆锛屼篃涓嶆槗娌夋穩铔嬬櫧銆備箼閰镐箼閰彁鍙栨湁鍒╀簬涓嬩竴姝ョ殑澶勭悊锛屼絾鏄彁鍙栫墿鐨勮剛鑲惈閲忚緝楂橈紝闄よ剛杩囩▼寰堝鏄撴崯澶辩洰鏍囩墿銆備箼鑵堢殑鎻愬彇鏁堢巼杈冮珮锛岃€屼笖鎻愬彇娑茬殑鑴傝偑鍚噺杈冨皯锛屽鐞嗕篃鐩稿鏂逛究銆備箼鑵堟彁鍙栨恫甯﹀叆鐨勫皯閲忚剛鑲彲浠ョ敤姝ｅ繁鐑峰幓闄わ紝灏介噺鍑忓皯鍩鸿川鐨勫共鎵帮紝鍥?涓哄噣鍖栧悗绌虹櫧鏍峰搧鍜屽姞鏍囨牱鍝佺殑鑹茶氨鍥俱€侟/span>

鍥? 11绉嶇：鑳虹┖鐧芥牱鍝?A)鍜屽姞鏍囨牱鍝?B)鐨勯珮鏁堟恫鐩歌壊璋卞浘

Fig.2 HPLC chromatograms of 11 sulfonamides for blank sample (A) and spiked sample (B) 1: Sulfadiazine, 2: Sulfathiazole, 3: Sulfamerazine, 4: Sulfapyridine, 5: Sulfachloropyridazine, 6: Sulfadoxine, 7: Sulfamethoxy-pyridazine, 8: Sulfameter, 9: Sulfisoxazole, 10: Sulfabenzamide, 11: Sulfaquinoxaline.

3.4 绾挎€ц寖鍥达紝妫€鍑洪檺鍜屽畾閲忛檺

閲囩敤绌虹櫧鍩鸿川娑查厤鍒剁郴鍒楁祿搴︾殑鏍囧噯婧舵恫锛屼互鍚勫垎鏋愮墿鐨勫嘲闈㈢Н(y)涓虹旱鍧愭爣锛岃川閲忔祿搴?x)涓烘í鍧愭爣缁樺埗鏍囧噯鏇茬嚎锛岀：鑳虹被鑽墿鍦?0锝?00渭g/L娴撳害鑼冨洿鍐呭憟鑹ソ鐨勭嚎鎬у叧绯伙紝鍚勫垎鏋愮墿鐨勭浉鍏崇郴鏁癛²>0.999銆備互3鍊嶄俊鍣瘮(S/N = 3)璁＄畻鏂规硶鐨勬鍑洪檺锛屼互10鍊嶄俊鍣瘮(S/N = 10)璁＄畻鏂规硶鐨勫畾閲忛檺锛屽叾涓：鑳哄鐢叉哀鍢у暥銆佺：鑳哄柟鍣佸晧鐨勬鍑洪檺鍜屽畾閲忛檺鍒嗗埆涓?.2鍜?.5渭g/kg锛涚：鑳哄槯鍟躲€佺：鑳哄櫥鍞戙€佺：鑳烘隘鍝掑棯銆佺：鑳哄悺鍟躲€佺：鑳虹敳姘у棯銆佺：鑳鸿嫰鐢查叞鐨勬鍑洪檺鍜屽畾閲忛檺鍒嗗埆涓?鍜?渭g/kg锛涚：鑳虹敳鍩哄槯鍟躲€佺：鑳轰簩鐢插熀鍢у暥銆佺：鑳轰簩鐢插紓鍣佸攽鐨勬鍑洪檺鍜屽畾閲忛檺涓?鍜?0渭g/kg锛岃瑙佽〃1銆侟/span>

琛 1 11绉嶇：鑳虹殑鍥炲綊鏂圭▼銆佺浉鍏崇郴鏁?R2)銆佹鍑洪檺鍜屽畾閲忛檺

Table 1 Regression equations, correlation coefficients, LOD and LOQ of 11 sulfonamides

纾鸿兒 Sulfonamides		鍥炲綊鏂圭▼ Regression equation	鐩稿叧绯绘暟 R2	妫€鍑洪檺 LOD* (渭g/kg)	瀹氶噺闄忺br/>LOQ* (渭g/kg)
	R
纾鸿兒鍢у暥 Sulfadiazine		y = 2017.62x - 61.89	0.9994	1	3
纾鸿兒鍣诲攽 Sulfathiazole		y = 1897.70x + 20.56	0.9995	1	3
纾鸿兒鐢插熀鍢у暥 Sulfamerazine		y = 1402.33x - 41.72	0.9994	3	10
纾鸿兒鍚″暥 Sulfapyridine		y = 11361.75x - 37.15	0.9992	1	3
纾鸿兒姘搾鍡猄ulfachloropyridazine		y = 8944.63x - 5.74	0.9998	1	3
纾鸿兒浜岀敳鍩哄槯鍟迭br/>Sulfadoxine		y = 2200.70x - 50.48	0.9991	3	10
纾鸿兒鐢叉哀鍡?br/>Sulfamethoxypyridazine		y = 4744.01x - 65.01	0.999	1	3
纾鸿兒瀵圭敳姘у槯鍟迭br/>Sulfameter		y = 15699.15x - 18.27	0.9998	0.2	0.5
纾鸿兒浜岀敳鍩哄紓鍣佸攽 Sulfisoxazole		y = 1456.02x - 29.74	0.9992	3	10
纾鸿兒鑻敳閰?br/>Sulfabenzamide		y = 2991.64x + 9.14	0.9994	1	3
纾鸿兒鍠瑰檨鍟堻br/>Sulfaquinoxaline		y = 12776.23x + 1.98	0.9995	0.2	0.5

* LOD: limit of detection, LOQ: limit of quantification.

3.5 鍥炴敹鐜囧拰绮惧瘑搴?/strong>

瀵归槾鎬х尓鑲夋牱鍝佽繘琛屽洖鏀剁巼瀹為獙锛屾坊鍔犳按骞冲垎鍒负瀹氶噺闄愮殑3鍊嶅拰5鍊嶏紝閲囩敤浼樺寲鍚庣殑鍓嶅鐞嗘柟娉曞鏍峰搧杩涜澶勭悊锛屾瘡涓坊鍔犳按骞抽噸澶?娆★紝鍚勭：鑳虹殑骞冲潎鍥炴敹鐜囧湪85.7%锝?01.1%涔嬮棿锛孯SD涓?.9%锝?.6%锛岃瑙佽〃2銆侟/span>

琛 2 鐚倝涓?1绉嶇：鑳虹殑鍔犳爣鍥炴敹鐜囩粨鏋?n = 6)

Table 2 Recoveries for the 11 sulfonamides in pork (n = 6)

纾鸿兒 Sulfonamides	鍔犳爣閲廃br/>Spiked (渭g/kg)	鍥炴敹鐜嘃br/>Recoveries (%)	RSD (%, n=6)	纾鸿兒 Sulfonamides	鍔犳爣閲廃br/>Spiked (渭g/kg)	鍥炴敹鐜嘃br/>Recoveries (%)	RSD (%, n=6)
纾鸿兒鍢у暥 Sulfadiazine	9 30	95.5 97.5	6.0 1.9	纾鸿兒鐢叉哀鍡?br/>Sulfamethoxypyridazine	9 30	96.1 101.1	6.6 5.3
纾鸿兒鍣诲攽 Sulfathiazole	9 30	98.2 98.5	3.7 3.8	纾鸿兒瀵圭敳姘у槯鍟迭br/>Sulfameter	1.5 5	87.9 89.4	3.8 5.0
纾鸿兒鐢插熀鍢у暥 Sulfamerazine	30 100	97.1 95.0	4.4 2.6	纾鸿兒浜岀敳鍩哄紓鍣佸攽 Sulfisoxazole	30 100	93.3 92.1	4.7 4.2
纾鸿兒鍚″暥 Sulfapyridine	9 30	85.7 87.2	3.5 3.4	纾鸿兒鑻敳閰?br/>Sulfabenzamide	9 30	93.8 90.7	3.8 4.0
纾鸿兒姘搾鍡?br/>Sulfachloropyridazine	9 30	96.2 96.6	2.5 5.6	纾鸿兒鍠瑰檨鍟堻br/>Sulfaquinoxaline	1.5 5	97.0 95.2	5.3 4.3
纾鸿兒浜岀敳鍩哄槯鍟迭br/>Sulfadoxine	30 100	95.3 93.0	3.2 5.4

3.6 瀹為檯鏍峰搧娴嬪畾

鍒╃敤鏈柟娉曟祴瀹氫簡璐簬鍐滆锤甯傚満鐨?0涓尓鑲夋牱鍝佷腑鐨勭：鑳虹被鑽墿娈嬬暀閲忋€傚湪10涓牱鍝佷腑锛屼粎鏈?渚嬫牱鍝佹鍑虹：鑳轰簩鐢插槯鍟讹紝娴嬪緱閲忎负24.5渭g/kg(n=3)銆傜粨鏋滆〃鏄庯紝鏈柟娉曠浉姣斾簬甯歌鍖栧璇曞墏琛嶇敓妫€娴嬫硶鍏锋湁缁胯壊銆佺幆淇濄€佺伒鏁忓害楂樸€佺ǔ瀹氭€уソ鐨勪紭鐐癸紝閫傜敤浜庤倢鑲夌粍缁囦腑纾鸿兒鑽墿娈嬬暀鐨勬娴嬨€侟/span>