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娣峰寑锛屽娓╅潤缃?0min锛岀敤钂搁姘磋皟闆讹紝660nm澶勮鍙栧悇绠″惛鍏夊€笺€侟/span> 娉ㄦ剰锛氳嫢鏈夋牱鏈祽娴婏紝寤鸿绂诲績鍚庡彇涓婃竻娴嬪畾銆侟/span> 涓夈€丼BE娲诲姏鍗曚綅璁$畻 1.鎸夌収铔嬬櫧娴撳害璁$畻鍗曚綅鐨勫畾涔夛細 浠ユ尝闀?60nm鐨勫惛鍏夊害涓嬮檷鐧惧垎鐜囪〃绀猴紝姣弇g铔嬬櫧鍦?ml鍙嶅簲浣撶郴涓瘡鍒嗛挓闄嶄綆1%纰樿摑鍊间负涓€涓叾娲绘€у崟浣嶃€侟/span> SBE娲绘€?U/mg prot) =(A瀵圭収绠?A娴嬪畾绠?梅A瀵圭収绠∶?00%梅1%梅(Cpr脳V鏍锋湰)脳V鍙嶅簲梅T =24脳(A瀵圭収绠?A娴嬪畾绠?梅A瀵圭収绠∶稢pr 2.鎸夌収鏍锋湰璐ㄩ噺璁$畻鍗曚綅鐨勫畾涔夛細 浠ユ尝闀?60nm鐨勫惛鍏夊害涓嬮檷鐧惧垎鐜囪〃绀猴紝姣廹缁勭粐鍦?ml鍙嶅簲浣撶郴涓瘡鍒嗛挓闄嶄綆1%纰樿摑鍊间负涓€涓叾娲绘€у崟浣嶃€侟/span> SBE娲绘€?U/g璐ㄩ噺) =(A瀵圭収绠?A娴嬪畾绠?梅A瀵圭収绠∶?00%梅1%梅(W梅V鎻愬彇脳V鏍锋湰)脳V鍙嶅簲梅T =(A瀵圭収绠?A娴嬪畾绠?梅A瀵圭収绠∶稺脳24 V鏍锋湰锛氬姞鍏ユ牱鏈綋绉紝0.25ml锛沄鎻愬彇锛氬姞鍏ユ彁鍙栨恫浣撶Н锛?ml锛汣pr锛氳泲鐧芥祿搴︼紝mg/ml锛沇锛氭牱鏈川閲忥紝g锛沄鍙嶅簲锛氬弽搴斾綋绯讳綋绉紝1.2ml锛汿锛氬弽搴旀椂闂达紝20min銆侟/span> 娉ㄦ剰浜嬮」锛欬/span> 1.鍙互鍦ㄤ笉鍚屽鐓х涓姞鍏ヤ笉鍚岀殑鏍锋湰锛岀劧鍚庨泦涓繘琛?min娌告按娴村鐞嗐€侟/span> 2.璇曞墏涓€濡傛湁娌夋穩锛屽姞鍏ヤ箣鍓嶈浣夸箣鍏呭垎婧惰В娣峰寑銆侟/span> |
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