姝ｅ紡娴嬪畾鍓嶅姟蹇呭彇 2-3涓鏈熷樊寮傝緝澶х殑鏍锋湰鍋氶娴嬪畾

娴嬪畾鎰忎箟锛欬/span>

钄楃硸杞寲閰?Invertase锛孖vr)鍌寲钄楃硸涓嶅彲閫嗗湴鍒嗚В涓烘灉绯栧拰钁¤悇绯栵紝鏄珮绛夋鐗╄敆绯栦唬璋㈠叧閿叾涔嬩竴銆傛牴鎹渶閫 pH锛孖vr鍒嗕负閰告€ц浆鍖栭叾(AI)鍜屼腑鎬ц浆鍖栭叾(NI)涓ょ绫诲瀷銆侟/span>

AI鐨勬渶閫 pH涓 3锝?銆侫I鍒嗕负鍙憾鎬 AI(S-AI)鍜岀粏鑳炲涓嶆憾鎬 AI(B-AI)涓ょ绫诲瀷銆 B-AI瀛樺湪浜庣粏鑳為棿闅欏苟缁撳悎鍦ㄧ粏鑳炲涓婏紝涓昏鍙備笌闊х毊閮ㄨ川澶栦綋鍗歌浇鏃惰敆绯栫殑鍒嗚В锛屼互缁存寔搴撴簮涔嬮棿钄楃硸鐨勬祿搴︺€侟/span>

娴嬪畾鍘熺悊锛欬/span>

B-AI鍌寲钄楃硸闄嶈В浜х敓杩樺師绯栵紝杩涗竴姝ヤ笌 3,5锛嶄簩纭濆熀姘存潹閰稿弽搴旓紝鐢熸垚妫曠孩鑹叉皑鍩哄寲鍚堢墿锛屽湪 510nm鏈夌壒寰佸厜鍚告敹锛屽湪涓€瀹氳寖鍥村唴 510nm鍏夊惛鏀跺鍔犻€熺巼涓 B-AI娲绘€ф垚姝ｆ瘮銆侟/span>

鑷鐢ㄥ搧锛欬/span>

鍙鍒嗗厜鍏夊害璁°€佸彴寮忕蹇冩満銆佹按娴撮攨銆佺Щ娑插櫒銆?mL鐜荤拑姣旇壊鐨裤€佺爺閽点€佸啺鍜岃捀棣忔按銆侟/span>

璇曞墏缁勬垚鍜岄厤鍒讹細

鎻愬彇娑 1锛氭恫浣 50mL脳1鐡讹紝4鈩冧繚瀛橈紱

鎻愬彇娑 2锛氭恫浣 50mL脳1鐡讹紝4鈩冧繚瀛橈紱

璇曞墏涓€锛氭恫浣 50mL脳1鐡讹紝4鈩冧繚瀛橈紱

璇曞墏浜岋細绮夊墏脳1鐡讹紝4鈩冧繚瀛橈紱涓寸敤鍓嶅姞鍏 25mL璇曞墏涓€鍏呭垎婧惰В澶囩敤锛涚敤涓嶅畬鐨勮瘯鍓 4鈩冧繚瀛橈紱

璇曞墏涓夛細娑蹭綋 30mL脳1鐡讹紝4鈩冧繚瀛橈紱

绮楅叾娑叉彁鍙栵細

鎸夌収缁勭粐璐ㄩ噺(g)锛氭彁鍙栨恫 1浣撶Н(mL)涓 1锛?锝?0鐨勬瘮渚?寤鸿绉板彇绾 0.1g缁勭粐锛屽姞鍏 1mL鎻愬彇娑 1)锛岃繘琛屽啺娴村寑娴嗐€?2000g 4鈩冪蹇 10min锛屽純涓婃竻锛屾矇娣€涓姞鍏 1mL钂搁姘达紝鍏呭垎闇囪崱娣峰寑锛?2000g 4鈩冪蹇 10min锛屽純涓婃竻锛屾矇娣€涓姞鍏 1mL鎻愬彇娑 2鍏呭垎娣峰寑锛?鈩冩蹈鎻愯繃澶滐紝12000g 4鈩冪蹇 20min锛屽彇涓婃竻缃啺涓婂緟娴嬨€侟/span>

娴嬪畾姝ラ鍜屽姞鏍疯〃锛欬/span>

璇曞墏鍚嶇О(渭L)	娴嬪畾绠狘/span>	瀵圭収绠狘/span>
鏍锋湰	200	200
璇曞墏涓€		800
璇曞墏浜孅/span>	800
娣峰寑锛 37鈩冨噯纭按娴 30min鍚庯紝95鈩冩按娴 10min(鐩栫揣锛屼互闃叉按鍒嗘暎澶?锛屾祦姘村喎鍗村悗鍏呭垎娣峰寑(浠ヤ繚璇佹祿搴︿笉鍙?
璇曞墏涓堻br/>	500	500

娣峰寑锛?5鈩冩按娴 10min(鐩栫揣锛屼互闃叉姘村垎鏁ｅけ)锛屾祦姘村喎鍗村悗鍏呭垎娣峰寑锛 510nm澶勶紝钂搁姘磋皟闆讹紝璁板綍鍚勭鍚稿厜鍊 A锛屽鏋滃惛鍏夊€煎ぇ浜 2锛屽彲浠ョ敤钂搁姘寸█閲婂悗娴嬪畾(璁＄畻鍏紡涓箻浠ョ浉搴旂█閲婂€嶆暟)锛屛擜=A娴嬪畾-A瀵圭収銆侟/span>

B-AI娲绘€ц绠楋細

鏍囧噯鏉′欢涓嬫祴瀹氱殑鍥炲綊鏂圭▼涓 y = 0.0016x -0.001锛泋涓烘爣鍑嗗搧娴撳害(渭g/mL)锛寉涓哄惛鍏夊€笺€侟/span>

(1)鎸夎泲鐧芥祿搴﹁绠楋細

鍗曚綅鐨勫畾涔夛細37鈩冩瘡 mg铔嬬櫧姣忓垎閽熶骇鐢 1渭g杩樺師绯栧畾涔変负涓€涓叾娲绘€у崟浣嶃€侟/span>

B-AI娲绘€?渭g/min/mg prot)锛漑(螖A +0.001)梅0.0016脳V1]梅(V1脳Cpr )梅T=20.8脳(螖A +0.001)梅Cpr

(2)鎸夐矞閲嶈绠楋細

鍗曚綅鐨勫畾涔夛細37鈩冩瘡 g缁勭粐姣忓垎閽熶骇鐢 1渭g杩樺師绯栧畾涔変负涓€涓叾娲绘€у崟浣嶃€侟/span>

B-AI娲绘€?渭g/min/g椴滈噸)锛漑(螖A +0.001)梅0.0016脳V1]梅(W 脳V1梅V2)梅T =20.8脳(螖A +0.001)梅W

V1锛氬姞鍏ュ弽搴斾綋绯讳腑鏍锋湰浣撶Н锛?.2mL锛沄2锛氬姞鍏ユ彁鍙栨恫浣撶Н锛?mL锛汿锛氬弽搴旀椂闂达紝30min锛 Cpr锛氭牱鏈泲鐧借川娴撳害锛宮g/mL锛沇锛氭牱鏈矞閲嶏紝g銆侟/span>