娉ㄦ剰锛氭寮忔祴瀹氫箣鍓嶉€夋嫨 2-3 涓鏈熷樊寮傚ぇ鐨勬牱鏈仛棰勬祴瀹氥€侟/span>

娴嬪畾鎰忎箟锛欬/span>

ACP鏄竴绉嶅湪閰告€х幆澧冧笅鍌寲铔嬬櫧璐ㄦ按瑙ｇ殑閰躲€傝閰朵富瑕佺敤浜庨厭绮惧彂閰点€佸暏閰掗吙閫犮€佹瘺鐨蒋鍖栥€佹灉閰掓緞娓呫€侀叡娌归吙閫犮€侀ゲ鏂欑瓑銆侟/span>

娴嬪畾鍘熺悊锛欬/span>

閰告€ф潯浠朵笅锛孉CP鍌寲閰泲鐧芥按瑙ｄ骇鐢熼叒姘ㄩ吀锛涘湪纰辨€ф潯浠朵笅锛岄叒姘ㄩ吀杩樺師纾烽捈閰稿寲鍚堢墿鐢熸垚閽ㄨ摑锛涢挩钃濆湪 680nm鏈夌壒寰佸惛鏀跺嘲锛岄€氳繃娴嬪畾鍏跺惛鍏夊害澧炲姞锛屾潵璁＄畻 ACP娲绘€с€侟/span>

鑷浠櫒鍜屾牱鍝侊細

姘存荡閿呫€佺鍔涙悈鎷屽櫒銆佸彲璋冨紡绉绘恫鏋€佸彲瑙佸垎鍏夊厜搴﹁/閰舵爣浠€佸井閲忕幓鐠冩瘮鑹茬毧/96瀛旀澘銆?.5 mL EP绠″拰钂搁姘淬€侟/span>

璇曞墏缁勬垚鍜岄厤缃細

娑蹭綋涓€锛?mL脳1鏀紝4鈩冧繚瀛樸€侟/span>

娑蹭綋浜岋細1mL脳1鏀紝4鈩冧繚瀛樸€侟/span>

璇曞墏涓€鐨勯厤鍒讹細涓寸敤鍓嶆寜娑蹭綋涓€锛氭恫浣撲簩锛氳捀棣忔按=90(渭L)锛欬strong>20(渭L)锛欬strong>21(mL)鐨勬瘮渚嬮厤鍒讹紝鐜伴厤鐜扮敤锛屽鍑虹幇鐧借壊绲姸娌夋穩鍒欎笉鑳界敤銆侟/span>

璇曞墏浜岋細绮夊墏脳1鐡讹紝4鈩冧繚瀛樸€備复鐢ㄥ墠鍔 4mL钂搁姘存憾瑙ｃ€侟/span>

璇曞墏涓夛細绮夊墏脳1鐡讹紝4鈩冮伩鍏変繚瀛樸€備复鐢ㄥ墠鍔犲叆 10mL璇曞墏涓€锛屾哺姘存荡涓鍔涙悈鎷屾憾瑙ｃ€?鍙湪鐑ф澂涓婄洊涓€灞備繚椴滆啘锛屾敞鎰忚瀵燂紝閬垮厤姘村垎鍏ㄩ儴钂稿彂锛屼竴鑸姞鐑 15-30鍒嗛挓锛岃璇曞墏涓鸿繃楗卞拰璇曞墏锛屽厖鍒嗘贩鍖€鍚庝粛鍑虹幇棰楃矑鐗╀笉婧剁墿涓嶅奖鍝嶄娇鐢?銆侟/span>

璇曞墏鍥涳細绮夊墏脳1鐡讹紝4鈩冧繚瀛樸€備复鐢ㄥ墠鍔犲叆 20mL钂搁姘存憾瑙ｃ€傝瘯鍓備簲锛氭恫浣 4mL脳1鐡讹紝4鈩冧繚瀛樸€侟/span>

鏍囧噯鍝侊細娑蹭綋 1mL脳1鏀紝0.25渭mol/mL鏍囧噯閰皑閰告憾娑诧紝4鈩冧繚瀛樸€侟/span>

绮楅叾娑叉彁鍙栵細

1銆佽瘯鍓備竴鐨勯厤鍒讹細瑙佽瘯鍓傜殑缁勬垚鍜岄厤鍒躲€侟/span>

2銆佺粍缁囷細鎸夌収缁勭粐璐ㄩ噺(g)锛氳瘯鍓備竴浣撶Н(mL)涓 1锛?锝?0鐨勬瘮渚?寤鸿绉板彇绾 0.1g缁勭粐锛屽姞鍏 1mL璇曞墏涓€)鍐版荡鍖€娴嗭紝8000g锛?鈩冪蹇 10min锛屽彇涓婃竻锛屽嵆绮楅叾娑层€侟/span>

3銆佽娓呮垨鍩瑰吇娑诧細鐩存帴娴嬪畾銆侟/span>

4銆佺粏鑿屻€佺湡鑿岋細鎸夌収缁嗚優鏁伴噺(10⁴涓?锛氳瘯鍓備竴浣撶Н(mL)涓 500锝?000锛?鐨勬瘮渚?寤鸿 500涓囩粏鑳炲姞鍏 1mL璇曞墏涓€)锛屽啺娴磋秴澹版尝鐮寸缁嗚優(鍔熺巼 300w锛岃秴澹 3绉掞紝闂撮殧 7绉掞紝鎬绘椂闂 3min)锛涚劧鍚 8000g锛?鈩冿紝绂诲績 10min锛屽彇涓婃竻缃簬鍐颁笂寰呮祴銆侟/span>

娴嬪畾鎿嶄綔锛欬/span>

1.鍒嗗厜鍏夊害璁?閰舵爣浠鐑 30min锛岃皟鑺傛尝闀垮埌 680 nm锛岃捀棣忔按璋冮浂銆侟/span>

2.璇曞墏浜屻€佽瘯鍓備笁鍜岃瘯鍓傚洓缃簬 30鈩冩按娴翠繚娓 30min銆侟/span>

3.瀵圭収绠★細鍙 0.5 mL EP绠★紝鍔犲叆 20渭L绮楅叾娑诧紝40渭L璇曞墏浜岋紝娣峰寑鍚庣疆浜 30鈩冩按娴翠繚娓?0min锛涘姞鍏 40渭L璇曞墏涓夛紝娣峰寑鍚 8000g锛?鈩冪蹇 10min锛涘彇 40渭L涓婃竻娑诧紝鍔犲叆鏂扮殑 EP绠★紝鍐嶅姞鍏 200渭L璇曞墏鍥涳紝40渭L璇曞墏浜旓紝娣峰寑鍚庣疆浜 30鈩冩按娴翠繚娓 20min锛屽彇 200渭L浜庡井閲忕幓鐠冩瘮鑹茬毧/96瀛旀澘锛?80nm娴嬪畾鍏夊惛鏀讹紝璁颁负 A瀵圭収绠°€侟/span>

4.娴嬪畾绠★細鍙 0.5 mL EP绠★紝鍔犲叆 20渭L绮楅叾娑诧紝40渭L璇曞墏涓夛紝娣峰寑鍚庣疆浜 30鈩冩按娴翠繚娓?0min锛涘姞鍏 40渭L璇曞墏浜岋紝娣峰寑鍚 8000g锛?鈩冪蹇 10min锛涘彇 40渭L涓婃竻娑诧紝鍔犲叆鏂扮殑 EP绠★紝鍐嶅姞鍏 200渭L璇曞墏鍥涳紝40渭L璇曞墏浜旓紝娣峰寑鍚庣疆浜 30鈩冩按娴翠繚娓 20min锛屽彇 200渭L浜庡井閲忕幓鐠冩瘮鑹茬毧/96瀛旀澘锛屼簬 680nm娴嬪畾鍏夊惛鏀讹紝璁颁负 A娴嬪畾绠°€?娉ㄦ剰涓庣┖鐧界涓嶅悓锛屽厛鍔犺瘯鍓備笁锛屽悗鍔犺瘯鍓備簩)

5.绌虹櫧绠★細鍙 0.5 mL EP绠★紝鍔犲叆 40渭L钂搁姘达紝200渭L璇曞墏鍥涳紝40渭L璇曞墏浜旓紝娣峰寑鍚庣疆浜?0鈩冩按娴翠繚娓 20min锛屽彇 200渭L浜庡井閲忕幓鐠冩瘮鑹茬毧/96瀛旀澘锛屼簬 680nm娴嬪畾鍏夊惛鏀讹紝璁颁负 A绌虹櫧绠°€侟/span>

6.鏍囧噯绠★細鍙 0.5 mL EP绠★紝鍔犲叆 40渭L鏍囧噯鍝侊紝200渭L璇曞墏鍥涳紝40渭L璇曞墏浜旓紝娣峰寑鍚庣疆浜?0鈩冩按娴翠繚娓 20min锛屽彇 200渭L浜庡井閲忕幓鐠冩瘮鑹茬毧/96瀛旀澘锛屼簬 680nm娴嬪畾鍏夊惛鏀讹紝璁颁负 A鏍囧噯绠°€侟/span>

ACP娲绘€ц绠楀叕寮忥細

1.鎸夌収鏍锋湰铔嬬櫧娴撳害璁＄畻

ACP娲绘€у崟浣嶅畾涔夛細30鈩冩瘡姣厠铔嬬櫧姣忓垎閽熸按瑙ｄ骇鐢 1nmol閰皑閰镐负 1涓叾娲诲崟浣嶃€侟/span>

ACP娲绘€?nmol/min/mg prot)= C鏍囧噯鍝伱?A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?脳V鍙嶆€幻?Cpr脳V1)梅T= 125脳(A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?梅Cpr

2锛庢寜鐓ф牱鏈川閲忚绠桙/span>

ACP娲绘€у崟浣嶅畾涔夛細30鈩冩瘡鍏嬫牱鍝佹瘡鍒嗛挓鍌寲姘磋В浜х敓 1 nmol閰皑閰镐负 1涓叾娲诲崟浣嶃€侟/span>

ACP娲绘€?nmol/min/g椴滈噸)=C鏍囧噯鍝伱?A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?脳V鍙嶆€幻?W脳V1梅V2)梅T= 125脳(A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?梅W

3.鎸夌収娑蹭綋浣撶Н璁＄畻

ACP娲绘€у崟浣嶅畾涔夛細30鈩冩瘡姣崌鏍峰搧姣忓垎閽熷偓鍖栨按瑙ｄ骇鐢 1nmol閰皑閰镐负 1涓叾娲诲崟浣嶃€侟/span>

ACP娲绘€?nmol/min/mL)=C鏍囧噯鍝伱?A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?脳V鍙嶆€幻稸1梅T= 125脳(A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?

1.鎸夌収缁嗚優鏁伴噺璁＄畻

ACP娲绘€у崟浣嶅畾涔夛細30鈩冩瘡 10⁴涓粏鑳炴瘡鍒嗛挓鍌寲姘磋В浜х敓 1nmol閰皑閰镐负 1涓叾娲诲崟浣嶃€侟/span>

ACP娲绘€?nmol/min/104 cell)=C鏍囧噯鍝伱?A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?脳V鍙嶆€幻?缁嗚優鏁伴噺脳V1梅V2)梅T= 125脳(A娴嬪畾绠★紞A瀵圭収绠?梅(A鏍囧噯绠★紞A绌虹櫧绠?梅缁嗚優鏁伴噺

C鏍囧噯鍝侊細0.25 渭 mol/mL鏍囧噯閰皑閰告憾娑诧紱V鍙嶆€伙細閰朵績鍙嶅簲鎬讳綋绉紝0.1mL锛汣pr锛氱矖閰舵恫铔嬬櫧璐ㄦ祿搴?mg/mL)锛沄1锛氬姞鍏ュ弽搴斾綋绯讳腑绮楅叾娑蹭綋绉?mL)锛?.02 mL锛沄2锛氭彁鍙栨恫鎬讳綋绉?mL)锛?mL锛汿锛氬偓鍖栧弽搴旀椂闂?min)锛?0min锛沇锛氭牱鍝佽川閲?g)銆侟/span>

娉ㄦ剰浜嬮」锛欬/span>

1.璇曞墏涓€鎸夋搷浣滄寚绀洪厤鍒讹紝鐢ㄥ灏戦厤澶氬皯锛屽嚭鐜扮櫧鑹茬诞鐘舵矇娣€鍒欎笉鑳界敤銆侟/span>

2.涓寸敤鍓嶉厤鍒剁殑璇曞墏閰嶇疆濂藉悗 3澶╁唴浣跨敤瀹屾瘯銆侟/span>