浜у搧浠嬬粛
| 绉嶅睘 |
浜虹被 |
| 骞撮緞(鎬у埆) |
鐢锋€э紝51宀?/td> |
| 缁勭粐鏉ユ簮 |
缁撶洿鑲犺吅鐧岋紝鏉ヨ嚜杞Щ娣嬪反缁掽/td> |
| 鐢熼暱鐗规€?/td> |
璐村缁嗚優 |
| 缁嗚優褰㈡€?/td> |
涓婄毊缁嗚優鏍饵/td> |
| 鐢熺墿瀹夊叏绛夌骇 |
1 |
| 鐢熼暱鍩瑰吇鍩裹/td> |
Leibovitz's L-15+10% FBS+1% P/S |
| 鎺ㄨ崘浼犱唬姣斾緥 |
1:3-1:4 |
| 鎺ㄨ崘鎹㈡恫棰戠巼 |
2锝?娆?鍛?/td> |
| 鍊嶅鏃堕棿 |
锝?0-26灏忔椂 |
| 鍐诲瓨鏉′欢 |
鍐诲瓨娑诧細55% 鍩虹鍩瑰吇鍩?40%FBS+5%DMSO娓╁害锛氭恫姘?/td> |
| 鍩瑰吇鏉′欢 |
姘旂浉锛氱┖姘旓紝100%娓╁害锛?7鈩傸/td> |
| 鑷寸槫鎬?/td> |
Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 1脳10^7 cells). |
| 鎶楀師琛ㄨ揪鎯呭喌 |
Blood Type A; Rh+ |
| 鍩哄洜琛ㄨ揪鎯呭喌 |
Carcinoembryonic antigen (CEA) 0.15 ng/10^6 cells/10 days; transforming growth factor alpha; matrilysin. The cells are negative for expression of CSAp (CSAp-) and colon antigen 3, negative. The cells are positive for keratin by immunoperoxidase staining. The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. |
| 娉ㄦ剰浜嬮」 |
1銆佽缁嗚優鎺ㄨ崘浣跨敤Leibovitz's L-15鍩瑰吇鍩鸿繘琛屽煿鍏伙紝Leibovitz's L-15涓嶅彲浠ラ€氬叆浜屾哀鍖栫⒊锛屼細浜х敓缁嗚優姣掓€э紱2銆佸鎮ㄦ病鏈夋棤浜屾哀鍖栫⒊鐨勫煿鍏荤锛屽彲浣跨敤DMEM鏇夸唬Leibovitz's L-15锛屼娇鐢―MEM鍩瑰吇鍩烘椂鍗冲彲姝ｅ父閫氬叆5%浜屾哀鍖栫⒊锛?銆侀厤濂椾笓鐢ㄥ煿鍏诲熀榛樿Leibovitz's L-15閰嶇疆锛屽彲閫塂MEM閰嶆柟銆侟/td> |
| 淇濊棌鏈烘瀯 |
ATCC; CCL-227 ECACC; 87051203 |
STR閴村畾
STR浣嶇偣淇℃伅
| Amelogenin |
X,X |
| CSF1PO |
13,14 |
| D2S1338 |
17,24 |
| D3S1358 |
16,16 |
| D5S818 |
13,13 |
| D7S820 |
8,9 |
| D8S1179 |
13,13 |
| D13S317 |
12,12 |
| D16S539 |
9,9 |
| D18S51 |
13,13 |
| D19S433 |
13,13 |
| D21S11 |
30,30.2 |
| FGA |
24,24 |
| PentaD |
9,15 |
| PentaE |
10,10 |
| TH01 |
8,8 |
| TPOX |
11,11 |
| vWA |
16,16 |
| D6S1043 |
11,12 |
| D12S391 |
17,17 |
| D2S441 |
10,15 |
STR閴村畾鍥近/p>
|
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鐩稿叧浜у搧
|
鍦ㄧ嚎鐣欒█
