种属	人类
年龄(性别)	女性，6个月
组织来源	外周血
生长特性	悬浮细胞
细胞形态	淋巴母细胞样
背景描述	Established from the peripheral blood of a 6-month-old girl with acute megakaryoblastic leukemia (AML M7) at diagnosis in 1987; subline of the growth factor-independent cell line M-07; M-07e cells respond proliferatively to GM-CSF, IFN-alpha, IFN-beta, IFN-gamma, IL-2, IL-3, IL-4, IL-6, IL-15, NGF, SCF, TNF-alpha, TPO; we have noted that the cell line can become very quickly cytokine-independent (within 3-4 weeks), presumably due to outgrowth of independent cells. Exome and RNA sequence data are available
生物安全等级	1
生长培养基	RPMI-1640+10% FBS+8ng/mL GM-CSF+1% P/S
推荐传代比例	5×105-1×106cells/mL
推荐换液频率	2～3次/周
倍增时间	～40小时
冻存条件	冻存液：55% 基础培养基+40%FBS+5%DMSO温度：液氮
培养条件	气相：空气，95%；CO2，5%温度：37℃
抗原表达情况	CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
注意事项	该细胞为悬浮细胞，请注意离心收集细胞悬液；请勿直接倒掉细胞培养液。
保藏机构	DSMZ;ACC-104

STR鉴定

STR位点信息

Amelogenin	X
CSF1PO	9,10
D2S1338	19,25
D3S1358	16,19
D5S818	11
D7S820	11
D8S1179	13,16
D13S317	10,11
D16S539	11
D18S51	15,20
D19S433	14,15

D21S11	30,32.2
FGA	18,22
PentaD	9
PentaE	12,14
TH01	6,8
TPOX	8
vWA	16,18
D6S1043	13,19
D12S391	23,24
D2S441	14

STR鉴定图