| Organism |
Tissue |
Morphology |
Culture Properties |
| Homo sapiens (Human) |
Urinary bladder |
Epithelial |
Adherent |
鏈粏鑳炴牚璇︾粏淇℃伅濡備笅:
| General Information |
| Cell Line Name |
RT112 (Human Bladder Carcinoma Cells) |
| Synonyms |
RT 112; RT-112 |
| Organism |
Homo sapiens (Human) |
| Tissue |
Urinary bladder |
| Cell Type |
Fibroblast |
| Morphology |
Epithelial |
| Disease |
Urinary bladder transitional cell carcinoma |
| Strain |
锛岦/td> |
| Biosafety Level* |
1 |
| Age at Sampling |
Age unspecified |
| Gender |
Female |
| Genetics |
锛岦/td> |
| Ethnicity |
锛岦/td> |
| Applications |
锛岦/td> |
| Category |
Cancer cell line |
* Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
| Characteristics |
| Karyotype |
锛岦/td> |
| Virus Susceptibility |
锛岦/td> |
| Derivation |
锛岦/td> |
| Clinical Data |
锛岦/td> |
| Antigen Expression |
锛岦/td> |
| Receptor Expression |
锛岦/td> |
| Oncogene |
锛岦/td> |
| Genes Expressed |
锛岦/td> |
| Gene expression databases |
ArrayExpress: E-MTAB-2706; E-MTAB-2770; E-MTAB-3610; GEO: GSM887552; GSM888635; GSM1374857; GSM1374858; GSM1574545; GSM1574581; GSM1670393 |
| Metastasis |
锛岦/td> |
| Tumorigenic |
锛岦/td> |
| Effects |
锛岦/td> |
| Comments |
锛岦/td> |
| Culture Method |
| Doubling Time |
24锝?5 hrs |
| Methods for Passages |
Wash by PBS once then 0.05% trypsin-EDTA solution and incubate at room temperature (or at 37oC), observe cells under an inverted microscope until cell layer is dispersed (usually within 1 to 5 minutes) |
| Medium |
MEM |
| Special Remarks |
锛岦/td> |
| Medium Renewal |
锛岦/td> |
| Subcultivation Ratio |
锛岦/td> |
| Growth Condition |
95% air + 5% CO2, 37oC |
| Freeze medium |
DMEM (high glucose)+20% FBS+10% DMSO锛屼篃鍙互璁㈣喘纰т簯澶╃殑缁嗚優鍐诲瓨娑?C0210)銆侟/td> |
鏈粏鑳炴牚缁忚繃鏀師浣撴娴?Mycoplasma Test)锛屾娴嬬粨鏋滀负闃存€с€侟br/>鏈粏鑳炴牚閫氳繃STR (short tandem repeats)閴村畾锛屽叿浣撶粨鏋滃涓嬶細
| AMEL |
D16S539 |
D7S820 |
|
|
|
| D5S818 |
THO1 |
TPOX |
|
|
|
| vWA |
D13S317 |
CSF1PO |
|
|
|
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