| 褰㈡€佺壒鎬?/td> |
涓婄毊缁嗚優鏍饵/td> |
| 鐢熼暱鐗规€?/td> |
璐村鐢熼暱 |
| 鐗瑰緛鐗规€?/td> |
璇ョ粏鑳?983骞村缓绯伙紝婧愯嚜68宀佽偤鑵虹檶濂虫€х殑杞Щ娣嬪反缁撱€侟/td> |
| 鍩瑰吇鏉′欢 |
DMEM/F12(1:1): Ham锛噑 F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium)5%FBS锛?.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30nM Sodium selenite + 10 nM Hydrocortisone + 10 nM beta-estradiol + extra 2mM L-glutamine |
| 浼犱唬鏂规硶 |
|
| 浼犱唬鎯呭喌 |
P25 |
| 鍐诲瓨鏉′欢 |
鍩虹鍩瑰吇鍩?8%DMSO+20%FBS |
| 鏀師浣撴娴婞/td> |
鍩瑰吇娉?-) |
| STR |
Amelogenin锛歑锛汣SF1PO锛?0锛?2锛汥13S317锛?2锛汥16S539锛?2锛汥18S51锛?4锛汥19S433锛?4锛?6锛?7锛汥21S11锛?9锛汥2S1338锛?6锛?9锛汥3S1358锛?6锛汥5S818锛?3锛汥7S820锛?锛?1锛汥8S1179锛?0锛?5锛汧GA锛?2锛汿H01锛?.3锛汿POX锛?锛泇WA锛?4锛汓/td> |
| 浣跨敤鏉冮檺 |
A绫狐/td> |
| 澶囨敞 |
1. 鍗忓拰缁嗚優璧勬簮涓績涓虹瀛︾爺绌舵彁渚涘疄楠岀粏鑳炴妧鏈湇鍔°€傛墍鎻愪緵鐨勫疄楠岀粏鑳炲強鍏跺瓙浠ｏ紝涓嶈兘鐢ㄤ簬浜轰綋瀹為獙鍜屼复搴婅瘖鏂€佹不鐤楋紝涓嶈兘鐩存帴鎴栭棿鎺ョ敤浜庡晢涓氳涓恒€?. 鍗忓拰缁嗚優璧勬簮涓績鎵胯灏芥渶澶у姫鍔涘瀹為獙缁嗚優璧勬簮鐩稿叧淇℃伅杩涜鍙婃椂鏇存柊銆備絾闄愪簬褰撴椂鐨勬妧鏈潯浠讹紝涓績涓嶄繚璇佸叾鍑嗙‘鎬с€?. 浠庢枃鐚瓑澶勫紩鐢ㄧ殑淇℃伅鏈腑蹇冩湭杩涜鏍稿疄锛屼粎渚涘弬鑰冦€?. 浣跨敤鑰呭湪鎺ユ敹銆佸鐞嗐€佷繚瀛樸€佷涪寮冦€佽浆璁╁強浣跨敤缁嗚優鐨勬椂鍊欒閬靛畧鍥藉唴澶栨湁鍏崇殑娉曞緥娉曡锛屽厖鍒嗚€冭檻鍙兘瀛樺湪鐨勯闄╁拰璐ｄ换锛岄噰鍙栭€傚綋鐨勫畨鍏ㄥ拰澶勭悊鎺柦灏介噺闄嶄綆瀵瑰仴搴锋垨鐜鐨勫嵄瀹炽€侟/td> |
| 淇濊棌鍗曚綅 |
涓浗鍖诲绉戝闄㈠熀纭€鍖诲鐮旂┒鎵€缁嗚優璧勬簮涓績 |
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