半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-520 |
标识符
Identifier |
CSTR:19375.09.3101MONSCSP520 |
描述
Description |
Vero细胞株是日本千叶大学Y. Yasumura和Y. Kawakita从正常成年非洲绿猴的肾脏建株的。1964年6月15日，B. Simizu将其从千叶大学带到美国国立卫生研究院(NIH)过敏及传染病研究所热带病毒实验室时，已传至第93代。该细胞是贴壁依赖性的成纤维细胞。它能支持多种病毒的增殖，包括乙型脑炎、脊髓灰质炎、狂犬病等病毒，已被准许用于生产人用病毒疫苗。
细胞质量控制：
1.支原体检测结果：每个批次均通过本库支原体检测，结果为阴性。
2.STR鉴定结果：
①该株细胞DNA进行小鼠细胞STR分型结果显示，扩增后图谱清晰，分型结果良好；
②该细胞为猴源Vero细胞，未发现存在人或其他猴源细胞污染。
3.种属鉴定结果：采用PCR引物扩增技术对该细胞株进行种属鉴定，结果显示为非洲绿猴细胞，且无其他常见物种细胞污染。 |
动物种别
Organism |
非洲绿猴 |
性别
Gender |
|
组织来源
Tissue and Cell Type |
肾脏 |
形态
Morphology |
上皮样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
非洲绿猴肾细胞Vero完全培养液配方(100 ml)：
MEM (Invitrogen, 11090081) 87 ml
FBS (Gibco) 10 ml
Glutamax(invitrogen 35050061) 1 ml
SODIUM PYRUVATE SOL(invitrogen 11360070) 1 ml
Non-essential Amino Acids, 100X (Invitrogen, 11140050) 1 ml
气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。
生物安全等级：BSL-1
参考传代周期：2～3天
参考传代比例：1：3～1：6
参考换液频率：每周2～3次
冻存液配方：完全培养液95%，DMSO 5% |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
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