半岛bd体育手机客户端 介绍
目录号
Serial |
SCSP-508 |
标识符
Identifier |
CSTR:19375.09.3101MONSCSP508 |
描述
Description |
SV40转化的非洲绿猴肾细胞COS-7。此细胞株源自CV-1细胞株，经带有编码野生型T抗原的起始失活突变的SV40转染得到。
细胞质控结果：
1.支原体检测结果：每个批次均通过本库支原体检测，结果为阴性。
2.种属鉴定结果：采用PCR引物扩增技术对该细胞株进行种属鉴定，结果显示为非洲绿猴细胞，且无其他常见物种细胞污染。 |
动物种别
Organism |
非洲绿猴 |
性别
Gender |
|
组织来源
Tissue and Cell Type |
肾脏 |
形态
Morphology |
成纤维细胞样，贴壁生长 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
COS-7 完全培养液 配方(100 ml)
DMEM (Invitrogen, 11960-044)88 ml
Glutamax (Invitrogen, 35050061) 1 ml
Sodium Pyruvate 100 mM Solution (Invitrogen, 11360070) 1 ml
FBS (Gibco) 10 ml
气相：空气，95%；二氧化碳，5%。温度：37摄氏度。
生物安全等级：BSL-2
参考传代周期：每周2-3次
参考传代比例：1：4～1：8
参考换液频率：每周2～3次
冻存液配方：完全培养液95%，DMSO 5% |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
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