细胞培养基 >> 细胞株
CHO/dhFr-仓鼠卵巢细胞,二氢叶酸还原酶缺陷
半岛bd体育手机客户端 介绍
目录号 Serial |
GNHa 4 |
标识符 Identifier |
CSTR:19375.09.3101HAMGNHa4 |
描述 Description |
该细胞为二氢叶酸还原酶缺陷细胞。 在没有HT(次黄嘌呤-胸腺嘧啶)时细胞会死亡。 细胞培液中加氨甲喋呤可以防止对药物低抗性的回变细胞的生长。 在本库通过支原体检测。 |
动物种别 Organism |
中国仓鼠 |
性别 Gender |
雌 |
组织来源 Tissue and Cell Type |
正常卵巢 |
形态 Morphology |
上皮细胞 |
培养基和添加剂 Complete Growth Medium and Culture Conditions |
IMDM培养基(GIBCO,货号12200036),90%;优质胎牛血清,10%;添加0.1mM次黄嘌呤,0.016mM胸腺嘧啶,100nM氨甲喋呤。 气相:空气,95%;二氧化碳,5%。 温度:37摄氏度。 |
供应限制 Permits and Restrictions |
A。仅供研究之用。 |
REFERENCE |
Shulman T , et al. An antibody reactive with domain 4 of the platelet-derived growth factor beta receptor allows BB binding while inhibiting proliferation by impairing receptor dimerization. J. Biol. Chem. 272: 17400-17404, 1997. PubMed: 9211881Morel-Kopp MC , et al. A three amino acid deletion in glycoprotein IIIa is responsible for type I glanzmann's thrombasthenia: importance of residues I1e325Pro326Gly327 for beta 3 integrin subunit association. Blood 90: 669-677, 1997. PubMed: 9226167Libyh MT , et al. A recombinant human scFv anti-Rh(D) antibody with multiple valences using a C-terminal fragment of C4-binding protein. Blood 90: 3978-3983, 1997. PubMed: 9354666 |
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