半岛bd体育手机客户端 介绍
目录号
Serial |
TCR 1 |
标识符
Identifier |
CSTR:19375.09.3101RATTCR1 |
描述
Description |
胶质细胞株C6是由Benda等用N-nitrosomethylurea诱导的大鼠胶质瘤克隆，并经过一系列的体外培养和动物传代交替后建成的。 当细胞从低密度生长到满瓶时，S-100产量增加10倍。
在本库通过支原体检测。 |
动物种别
Organism |
大鼠 |
性别
Gender |
*** |
组织来源
Tissue and Cell Type |
胶质瘤，脑，胶质细胞 |
形态
Morphology |
成纤维细胞 |
培养基和添加剂
Complete Growth Medium and Culture Conditions |
F-12K Nutrient Mixture (gibco by life technologies 21127-022)，82.5%；马血清，15%；优质胎牛血清，2.5%。
气相：空气，95%；二氧化碳，5%。
温度：37摄氏度。 |
供应限制
Permits and Restrictions |
A。仅供研究之用。 |
| REFERENCE |
Science 161: 370-371, 1968. PubMed: 4873531Fed. Proc. 27: 720, 1968.J. Virol. 70: 8765-8772, 1996. PubMed: 8971005Reznikoff CA , et al. Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division. Cancer Res. 33: 3239-3249, 1973. PubMed: 4796800Terzaghi M , Little JB . Repair of potentially lethal radiation damage in mammalian cells is associated with enhancement of malignant transformation. Nature 253: 548-549, 1975. PubMed: 1167940Mondal S , Heidelberger C . Transformation of C3H/10T1/2 CL8 mouse embryo fibroblasts by ultraviolet irradiation and a phorbol ester. Nature 260: 710-711, 1976. PubMed: 1264242Smith GJ , et al. Clonal analysis of the expression of multiple transformation phenotypes and tumorigenicity by morphologically transformed 10T1/2 cells. Cancer Res. 53: 500-508, 1993. PubMed: 8425183Rapp UR , et al. Endogenous oncornaviruses in chemically induced transformation. I. Transformation independent of virus production. Virology 65: 392-409, 1975. PubMed: 165619Reznikoff CA , et al. Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division. Cancer Res. 33: 3231-3238, 1973. PubMed: 4357355Jain MK , et al. Molecular cloning and characterization of SmLIM, a developmentally regulated LIM protein preferentially expressed in aortic smooth muscle cells. J. Biol. Chem. 271: 10194-10199, 1996. PubMed: 8626582Chinese Journal of Cancer 21(10):1106-1108, 2002J Cancer Prev Treat 29(4):280-282,2002Natl Med J China 82(2):90-93,2002Jiangsu Med J. 28(S1015):343-345,2002Chinese Journal of Cancer 22(9):959-963,2003Research in Tumor Prev Treat 30(3):176-179,2003Research in Tumor Prev Treat 30(5):357-358,2003China J Cancer Prev Treat 10(5):490-492,2003Journal of Shandong University (Health Sciences) 41(4):394-396,2003J Fujian Med Univ 37(3):269-272,2003 |
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